RU.521 (Synonyms: RU320521)

Name: RU.521
Brand: MedChemExpress
SKU: HY-114180
Rating: 5.0 (22 reviews)

Cat. No.: HY-114180 Purity: 99.89%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

RU.521 (RU320521) is a potent and selective cyclic GMP-AMP synthase (cGAS) inhibitor and inhibits cGAS-mediated interferon upregulation. RU.521 suppresses dsDNA-activated reporter activity with an IC₅₀ of 0.7 μM. RU.521 reduces constitutive expression of interferon in macrophages from a mouse model of Aicardi-Goutières syndrome (AGS).

For research use only. We do not sell to patients.

RU.521 Chemical Structure

CAS No. : 2262452-06-0

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 132	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 132	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 210	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 490	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 850	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 22 publication(s) in Google Scholar

21 Publications Citing Use of MCE RU.521

: RU.521 purchased from MedChemExpress. Usage Cited in: Toxicol Lett. 2022 Nov 8;S0378-4274(22)01779-9. [Abstract]; RU.521 (2.4 μM) significantly reduces the elevation of cGAS and STING triggered by ZnONPs in BEAS-2B cells.

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Description

IC₅₀ & Target

IC50: 0.7 μM (dsDNA)^[1]

In Vitro

RU.521 (0.1 nM-1000 μM; 72 h) suppresses dsDNA-induced signaling in macrophage cells^[1].
RU.521(0-100 μM; 24 h) selectively inhibits cGAS-mediated signaling^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

RU.521 (5 mg/kg ; i.p. once) reduces symptoms from sepsis in mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male 8-week-old male mice with LPS injection^[2]
Dosage:	5 mg/kg
Administration:	Intraperitoneal injection; 5 mg/kg once
Result:	Increased cardiac function and reduced the inflammatory responses, oxidative stress and apoptosis in hearts of mice.

Molecular Weight

415.23

Formula

C₁₉H₁₂Cl₂N₄O₃

CAS No.

2262452-06-0

Appearance

Solid

Color

Off-white to yellow

SMILES

O=C1OC(C2=C(O)N(C3=NC4=CC=C(Cl)C(Cl)=C4N3)N=C2C)C5=C1C=CC=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 83.33 mg/mL (200.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4083 mL	12.0415 mL	24.0830 mL
5 mM	0.4817 mL	2.4083 mL	4.8166 mL
10 mM	0.2408 mL	1.2042 mL	2.4083 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.89%

Select Batch:

Data Sheet (276 KB) SDS (0 KB)

COA (251 KB) HNMR (255 KB) LCMS (266 KB)

Handling Instructions (2659 KB)

References

[1]. Vincent J, et al. Small molecule inhibition of cGAS reduces interferon expression in primary macrophages from autoimmune mice. Nat Commun. 2017 Sep 29;8(1):750. [Content Brief]

[2]. Xu Q, et al. Small molecule inhibition of cyclic GMP-AMP synthase ameliorates sepsis-induced cardiac dysfunction in mice. Life Sci. 2020 Nov 1;260:118315. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4083 mL	12.0415 mL	24.0830 mL	60.2076 mL
	5 mM	0.4817 mL	2.4083 mL	4.8166 mL	12.0415 mL
	10 mM	0.2408 mL	1.2042 mL	2.4083 mL	6.0208 mL
	15 mM	0.1606 mL	0.8028 mL	1.6055 mL	4.0138 mL
	20 mM	0.1204 mL	0.6021 mL	1.2042 mL	3.0104 mL
	25 mM	0.0963 mL	0.4817 mL	0.9633 mL	2.4083 mL
	30 mM	0.0803 mL	0.4014 mL	0.8028 mL	2.0069 mL
	40 mM	0.0602 mL	0.3010 mL	0.6021 mL	1.5052 mL
	50 mM	0.0482 mL	0.2408 mL	0.4817 mL	1.2042 mL
	60 mM	0.0401 mL	0.2007 mL	0.4014 mL	1.0035 mL
	80 mM	0.0301 mL	0.1505 mL	0.3010 mL	0.7526 mL
	100 mM	0.0241 mL	0.1204 mL	0.2408 mL	0.6021 mL

RU.521 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RU.5212262452-06-0RU320521RU 320521RU-320521Cyclic GMP-AMP SynthasecGASsignaling pathwayoxidative stressinflammatoryInhibitorinhibitorinhibit

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RU.521 (Synonyms: RU320521)

Customer Review

RU.521 Related Antibodies

21 Publications Citing Use of MCE RU.521

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Complete Stock Solution Preparation Table

RU.521 Related Classifications

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