Affinity DataKi: 0.300nM Kd: 0.100nMAssay Description:Assay of DOT1L enzymatic activity were performed under balanced conditions using a radiometric assay.More data for this Ligand-Target Pair
Affinity DataKi: 0.300nM Kd: 0.0100nM Kon: 0.000300M-1s-1 Koff: 3.00E+6s-1Assay Description:Unless otherwise indicated, assays of DOT1L enzymatic activity were performed under balanced conditions (all substrates present at concentrations equ...More data for this Ligand-Target Pair
Affinity DataKi: 4nMAssay Description:Assay of DOT1L enzymatic activity were performed under balanced conditions using a radiometric assay.More data for this Ligand-Target Pair
Affinity DataKi: 13nMAssay Description:Assay of DOT1L enzymatic activity were performed under balanced conditions using a radiometric assay.More data for this Ligand-Target Pair
Affinity DataKi: 13nM Kd: 1.70nM Kon: 0.0200M-1s-1 Koff: 1.20E+7s-1Assay Description:Unless otherwise indicated, assays of DOT1L enzymatic activity were performed under balanced conditions (all substrates present at concentrations equ...More data for this Ligand-Target Pair
Affinity DataKi: 320nM Kd: 71nMAssay Description:Assay of DOT1L enzymatic activity were performed under balanced conditions using a radiometric assay.More data for this Ligand-Target Pair
Affinity DataKi: 320nM Kd: 71nM Kon: 0.100M-1s-1 Koff: 1.40E+6s-1Assay Description:Unless otherwise indicated, assays of DOT1L enzymatic activity were performed under balanced conditions (all substrates present at concentrations equ...More data for this Ligand-Target Pair
Affinity DataKi: 845nM Kd: 167nM Kon: 0.200M-1s-1 Koff: 1.20E+6s-1Assay Description:Unless otherwise indicated, assays of DOT1L enzymatic activity were performed under balanced conditions (all substrates present at concentrations equ...More data for this Ligand-Target Pair
Affinity DataKi: 845nM Kd: 167nMAssay Description:Assay of DOT1L enzymatic activity were performed under balanced conditions using a radiometric assay.More data for this Ligand-Target Pair
Affinity DataKi: 1.20E+4nMAssay Description:Assay of DOT1L enzymatic activity were performed under balanced conditions using a radiometric assay.More data for this Ligand-Target Pair
Affinity DataKi: 2.00E+4nMAssay Description:Assay of DOT1L enzymatic activity were performed under balanced conditions using a radiometric assay.More data for this Ligand-Target Pair
Affinity DataKi: 3.80E+4nMAssay Description:Assay of DOT1L enzymatic activity were performed under balanced conditions using a radiometric assay.More data for this Ligand-Target Pair
TargetHistone-lysine N-methyltransferase, H3 lysine-79 specific [1-416](Homo sapiens (Human))
Epizyme
US Patent
Epizyme
US Patent
Affinity DataIC50: 0.590nMAssay Description:An in vitro biological assay that can be used includes the steps of (1) mixing a histone substrate (e.g., an isolated histone sample for a histone or...More data for this Ligand-Target Pair
TargetHistone-lysine N-methyltransferase, H3 lysine-79 specific [1-416](Homo sapiens (Human))
Epizyme
US Patent
Epizyme
US Patent
Affinity DataIC50: 0.590nMAssay Description:An in vitro biological assay that can be used includes the steps of (1) mixing a histone substrate (e.g., an isolated histone sample for a histone or...More data for this Ligand-Target Pair
Affinity DataIC50: 0.590nMAssay Description:Unless otherwise indicated, assays of DOT1L enzymatic activity were performed under balanced conditions (all substrates present at concentrations equ...More data for this Ligand-Target Pair
TargetHistone-lysine N-methyltransferase, H3 lysine-79 specific [1-416](Homo sapiens (Human))
Epizyme
US Patent
Epizyme
US Patent
Affinity DataIC50: 0.620nMAssay Description:An in vitro biological assay that can be used includes the steps of (1) mixing a histone substrate (e.g., an isolated histone sample for a histone or...More data for this Ligand-Target Pair
TargetHistone-lysine N-methyltransferase, H3 lysine-79 specific [1-416](Homo sapiens (Human))
Epizyme
US Patent
Epizyme
US Patent
Affinity DataIC50: 0.730nMAssay Description:An in vitro biological assay that can be used includes the steps of (1) mixing a histone substrate (e.g., an isolated histone sample for a histone or...More data for this Ligand-Target Pair
Affinity DataIC50: 0.730nMAssay Description:Unless otherwise indicated, assays of DOT1L enzymatic activity were performed under balanced conditions (all substrates present at concentrations equ...More data for this Ligand-Target Pair
TargetHistone-lysine N-methyltransferase, H3 lysine-79 specific [1-416](Homo sapiens (Human))
Epizyme
US Patent
Epizyme
US Patent
Affinity DataIC50: 0.740nMAssay Description:An in vitro biological assay that can be used includes the steps of (1) mixing a histone substrate (e.g., an isolated histone sample for a histone or...More data for this Ligand-Target Pair
Affinity DataIC50: 0.740nMAssay Description:Test compounds were serially diluted 3 fold in DMSO for 10 points and 1 μl was plated in a 384 well microtiter plate. Positive control (100% inh...More data for this Ligand-Target Pair
Affinity DataIC50: 0.740nMAssay Description:Unless otherwise indicated, assays of DOT1L enzymatic activity were performed under balanced conditions (all substrates present at concentrations equ...More data for this Ligand-Target Pair
TargetHistone-lysine N-methyltransferase, H3 lysine-79 specific [1-416](Homo sapiens (Human))
Epizyme
US Patent
Epizyme
US Patent
Affinity DataIC50: 0.800nMAssay Description:An in vitro biological assay that can be used includes the steps of (1) mixing a histone substrate (e.g., an isolated histone sample for a histone or...More data for this Ligand-Target Pair
TargetHistone-lysine N-methyltransferase, H3 lysine-79 specific [1-416](Homo sapiens (Human))
Epizyme
US Patent
Epizyme
US Patent
Affinity DataIC50: 2.18nMAssay Description:An in vitro biological assay that can be used includes the steps of (1) mixing a histone substrate (e.g., an isolated histone sample for a histone or...More data for this Ligand-Target Pair
TargetHistone-lysine N-methyltransferase, H3 lysine-79 specific [1-416](Homo sapiens (Human))
Epizyme
US Patent
Epizyme
US Patent
Affinity DataIC50: 2.51nMAssay Description:An in vitro biological assay that can be used includes the steps of (1) mixing a histone substrate (e.g., an isolated histone sample for a histone or...More data for this Ligand-Target Pair
TargetHistone-lysine N-methyltransferase, H3 lysine-79 specific [1-416](Homo sapiens (Human))
Epizyme
US Patent
Epizyme
US Patent
Affinity DataIC50: 2.92nMAssay Description:An in vitro biological assay that can be used includes the steps of (1) mixing a histone substrate (e.g., an isolated histone sample for a histone or...More data for this Ligand-Target Pair
Affinity DataIC50: 3nMAssay Description:Inhibition of PRMT5 in human Z138 cells assessed as reduction in symmetrical dimethylation of arginine containing substrate using SmD3 as substrate i...More data for this Ligand-Target Pair
TargetMethylosome protein 50/Protein arginine N-methyltransferase 5(Homo sapiens (Human))
Epizyme
Curated by ChEMBL
Epizyme
Curated by ChEMBL
Affinity DataIC50: 4nMAssay Description:Inhibition of human full length FLAG-tagged PRMT5/human His6-tagged MEP50 expressed in baculovirus-infected Sf9 cells assessed as reduction in tritiu...More data for this Ligand-Target Pair
Affinity DataIC50: 9nMAssay Description:Inhibition of PRMT5 in human Z138 cells assessed as reduction in symmetrical dimethylation of arginine containing substrate using SmD3 as substrate i...More data for this Ligand-Target Pair
Affinity DataIC50: 10nMAssay Description:Inhibition of methyltransferase activity of human EZH2 using chicken oligonucleotide as substrate by pull down assayMore data for this Ligand-Target Pair
Affinity DataIC50: 10nMAssay Description:Inhibition of methyltransferase activity of human EZH2 using chicken oligonucleotide as substrate by pull down assayMore data for this Ligand-Target Pair
Affinity DataIC50: 10nMAssay Description:Inhibition of methyltransferase activity of human EZH2 using chicken oligonucleotide as substrate by pull down assayMore data for this Ligand-Target Pair
TargetMethylosome protein 50/Protein arginine N-methyltransferase 5(Homo sapiens (Human))
Epizyme
Curated by ChEMBL
Epizyme
Curated by ChEMBL
Affinity DataIC50: 11nMAssay Description:Inhibition of human full length FLAG-tagged PRMT5/human His6-tagged MEP50 expressed in baculovirus-infected Sf9 cells assessed as reduction in tritiu...More data for this Ligand-Target Pair
Affinity DataIC50: 13nMAssay Description:Inhibition of PRMT5 in human Z138 cells assessed as reduction in symmetrical dimethylation of arginine containing substrate using SmD3 as substrate i...More data for this Ligand-Target Pair
TargetMethylosome protein 50/Protein arginine N-methyltransferase 5(Homo sapiens (Human))
Epizyme
Curated by ChEMBL
Epizyme
Curated by ChEMBL
Affinity DataIC50: 13nMAssay Description:Inhibition of human full length FLAG-tagged PRMT5/human His6-tagged MEP50 expressed in baculovirus-infected Sf9 cells assessed as reduction in tritiu...More data for this Ligand-Target Pair
TargetMethylosome protein 50/Protein arginine N-methyltransferase 5(Homo sapiens (Human))
Epizyme
Curated by ChEMBL
Epizyme
Curated by ChEMBL
Affinity DataIC50: 13nMAssay Description:Inhibition of human full length FLAG-tagged PRMT5/human His6-tagged MEP50 expressed in baculovirus-infected Sf9 cells assessed as reduction in tritiu...More data for this Ligand-Target Pair
Affinity DataIC50: 16nMAssay Description:Inhibition of PRMT5 in human Z138 cells assessed as reduction in symmetrical dimethylation of arginine containing substrate using SmD3 as substrate i...More data for this Ligand-Target Pair
TargetMethylosome protein 50/Protein arginine N-methyltransferase 5(Homo sapiens (Human))
Epizyme
Curated by ChEMBL
Epizyme
Curated by ChEMBL
Affinity DataIC50: 18nMAssay Description:Inhibition of human full length FLAG-tagged PRMT5/human His6-tagged MEP50 expressed in baculovirus-infected Sf9 cells assessed as reduction in tritiu...More data for this Ligand-Target Pair
Affinity DataIC50: 20nMAssay Description:Inhibition of methyltransferase activity of human EZH2 using chicken oligonucleotide as substrate by pull down assayMore data for this Ligand-Target Pair
TargetMethylosome protein 50/Protein arginine N-methyltransferase 5(Homo sapiens (Human))
Epizyme
Curated by ChEMBL
Epizyme
Curated by ChEMBL
Affinity DataIC50: 22nMAssay Description:Inhibition of human full length FLAG-tagged PRMT5/human His6-tagged MEP50 expressed in baculovirus-infected Sf9 cells assessed as reduction in tritiu...More data for this Ligand-Target Pair
TargetMethylosome protein 50/Protein arginine N-methyltransferase 5(Homo sapiens (Human))
Epizyme
Curated by ChEMBL
Epizyme
Curated by ChEMBL
Affinity DataIC50: 23nMAssay Description:Inhibition of human full length FLAG-tagged PRMT5/human His6-tagged MEP50 expressed in baculovirus-infected Sf9 cells assessed as reduction in tritiu...More data for this Ligand-Target Pair
Affinity DataIC50: 30nMAssay Description:Inhibition of methyltransferase activity of human EZH2 using chicken oligonucleotide as substrate by pull down assayMore data for this Ligand-Target Pair
Affinity DataIC50: 30nMAssay Description:Inhibition of methyltransferase activity of human EZH2 using chicken oligonucleotide as substrate by pull down assayMore data for this Ligand-Target Pair
Affinity DataIC50: 30nMAssay Description:Inhibition of methyltransferase activity of human EZH2 using chicken oligonucleotide as substrate by pull down assayMore data for this Ligand-Target Pair
Affinity DataIC50: 30nMAssay Description:Inhibition of methyltransferase activity of human EZH2 using chicken oligonucleotide as substrate by pull down assayMore data for this Ligand-Target Pair
Affinity DataIC50: 32nMAssay Description:Inhibition of PRMT5 in human Z138 cells assessed as reduction in symmetrical dimethylation of arginine containing substrate using SmD3 as substrate i...More data for this Ligand-Target Pair
TargetMethylosome protein 50/Protein arginine N-methyltransferase 5(Homo sapiens (Human))
Epizyme
Curated by ChEMBL
Epizyme
Curated by ChEMBL
Affinity DataIC50: 33nMAssay Description:Inhibition of human full length FLAG-tagged PRMT5/human His6-tagged MEP50 expressed in baculovirus-infected Sf9 cells assessed as reduction in tritiu...More data for this Ligand-Target Pair
Affinity DataIC50: 36nMAssay Description:Inhibition of PRMT5 in human Z138 cells assessed as reduction in symmetrical dimethylation of arginine containing substrate using SmD3 as substrate i...More data for this Ligand-Target Pair
Affinity DataIC50: 37nMAssay Description:Inhibition of PRMT5 in human Z138 cells assessed as reduction in symmetrical dimethylation of arginine containing substrate using SmD3 as substrate i...More data for this Ligand-Target Pair
Affinity DataIC50: 50nMAssay Description:Inhibition of methyltransferase activity of human EZH2 using chicken oligonucleotide as substrate by pull down assayMore data for this Ligand-Target Pair
Affinity DataIC50: 50nMAssay Description:Inhibition of methyltransferase activity of human EZH2 using chicken oligonucleotide as substrate by pull down assayMore data for this Ligand-Target Pair