Found 55 Enz. Inhib. hit(s) with Target = 'Serine/threonine-protein kinase B-raf' and Ligand = 'BDBM16673' TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
3D Structure (crystal)TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataKi: 38nM ΔG°: -44.1kJ/molepH: 7.0 T: 2°CAssay Description:BRAF kinase activity was quantified using an ELISA-based MEK phosphorylation assay. IC50 values were derived from the sigmoidal dose-response curve f...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 4.40nMAssay Description:Inhibition of BRAF V600E mutant in human A375 cells assessed as reduction in ERK phosphorylationMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 12nMAssay Description:Inhibition of purified B-Raf (unknown origin) after 30 mins by homogeneous time-resolved fluorescence assayMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 12nMAssay Description:Inhibition of B-Raf (unknown origin) using poly (Glu, Tyr) 4:1 substrate incubated for 30 mins by HTRF assayMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 15nMAssay Description:Inhibition of B-RAF kinaseMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 22nMAssay Description:Inhibition of B-Raf (unknown origin) using MEK1 as substrate measured after 2 hrs by ELISAMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 22nMAssay Description:Inhibition of recombinant BRAF (409 to 765 residues) (unknown origin) expressed in baculovirus expression system using MEk1 as substrate after 25 min...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 22nMAssay Description:Inhibition of recombinant BRAF (409 to 765 residues) (unknown origin) using MEK1 as substrate after 25 mins in presence of gamma-[33P]ATPMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 22nMAssay Description:Inhibition of B-Raf (unknown origin)More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 22nMAssay Description:Inhibition of BRAF (unknown origin)More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 22nMAssay Description:Inhibition of B-Raf kinaseMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 22nMAssay Description:Inhibition of B-RAF (unknown origin)More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 22nMAssay Description:Inhibition of wild type B-Raf assessed as inhibition of MEK1 phosphorylation using [gamma-33P]ATP by beta countingMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 22nMAssay Description:Inhibition of B-Raf in presence of 5 uM ATPMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 23nMAssay Description:Inhibition of BRAF (unknown origin)More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 23nMAssay Description:Inhibition of wild-type BRAF (unknown origin) using human His6-tagged MEK1 K97R mutant as substrate pretreated for 20 mins followed by [33P]-ATP addi...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 24nMAssay Description:Inhibition of B-Raf V600E mutant (unknown origin) using MEK1 as substrate measured after 2 hrs by ELISAMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 25nMAssay Description:Inhibition of BRAF (unknown origin)More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 26nMAssay Description:Inhibition of human GST-tagged full length B-Raf V600E mutant (unknown origin) using His6-tagged full-length human MEK1 (K97R) substrate and [33P]-ga...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 30nMAssay Description:Inhibition of human wild type BRAF by Kinase-Glo luminescence assayMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 38nMAssay Description:Inhibition of human GST-tagged BRAF V600E mutant (416 to 766 residues) using human full length 6His-tagged MEK1 (K97R) as substrate preincubated for ...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 38nMAssay Description:Inhibition of B-Raf (unknown origin)More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 38nMAssay Description:Inhibition of BRAF V600E mutant (unknown origin) measured after 40 mins by scintillation countingMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 38nMAssay Description:Inhibition of BRAF V600E (unknown origin)More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 38nMAssay Description:Inhibition of BRAF (unknown origin) incubated for 15 mins by fluorescence based assayMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 38nMAssay Description:Inhibition of recombinant BRAF (409 to 765 residues) V599E mutant (unknown origin) expressed in baculovirus expression system using MEk1 as substrate...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 41nMAssay Description:1. Preparation of test compounds: according to the molecular weight of the compounds, an appropriate volume of DMSO was directly added to dissolve th...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 43nMAssay Description:Inhibition of B-Raf (unknown origin) after 1 hr by ADP-Glo assayMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 43nMAssay Description:Inhibition of BRAF V600E mutantMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 46nMAssay Description:Inhibitory activity against human B-RAFMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 52nMAssay Description:Inhibition of BRAF V600E mutant (unknown origin) by Z'-Lyte assayMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 65nMAssay Description:Inhibition of BRAF V600E mutant (unknown origin)More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 65nMAssay Description:Inhibition of recombinant human N-terminal GST-tagged BRAF V600E mutant catalytic domain (416 to 766 residues) expressed in baculovirus expression sy...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 76.2nMAssay Description:Inhibition of human recombinant full-length GST-tagged B-Raf by radiometric kinase assayMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 114nMpH: 7.5 T: 2°CAssay Description:Compounds were prepared in DMSO at 10mmol/L and serially diluted in a 96-well plate as the source plate. The intermediate plate consists of 4µL...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 120nMAssay Description:Competitive inhibition of recombinant B-Raf (unknown origin) expressed in HEK293 cells by FRET analysis in presence of ATPMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 120nMAssay Description:Binding affinity to B-RAF kinase (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 130nMAssay Description:Inhibition of mouse full length GST-tagged BRAF V600E mutant using recombinant human full length N-terminal His-tagged MEK1 as substrate preincubated...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 136nMAssay Description:Competitive binding affinity to B-RAF kinase (unknown origin) after 60 to 120 mins by FRET analysisMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 310nMAssay Description:Inhibition of BRAF (unknown origin) using FAM-labeled peptide as substrate after 1 hr by Lanthascreen assayMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 310nMAssay Description:Inhibition of BRAF (unknown origin) incubated for 10 mins using FAM-labeled peptide and ATP by mobility shift assayMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 6.10E+3nMAssay Description:Growth inhibition in human melanoma cells WM266.4 expressing mutant B-RAF with SRBMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 6.60E+3nMAssay Description:Inhibition of BRAF V600E mutant-dependent MEK phosphorylation in A375 cellsMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 7.30E+3nMAssay Description:Inhibition of B-Raf in the presence of 100uM ATPMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataKd: 540nMAssay Description:Kinase inhibitors are a new class of therapeutics with a propensity to inhibit multiple targets. The biological consequences of multi-kinase activity...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataKd: 260nMAssay Description:Kinase inhibitors are a new class of therapeutics with a propensity to inhibit multiple targets. The biological consequences of multi-kinase activity...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataKd: 284nMAssay Description:Binding affinity to BRAF V600E mutantMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataKd: 540nMAssay Description:Binding constant for BRAF kinase domainMore data for this Ligand-Target Pair
