Affinity DataKd: 2.20E+5nMpH: 7.4 T: 2°CAssay Description:NMR measurements consisted of monitoring changes in chemical shifts and line widths of the backbone amide resonances of uniformly 15N-enriched MDM2 s...More data for this Ligand-Target Pair
Affinity DataKd: 9.00E+4nMpH: 7.4 T: 2°CAssay Description:NMR measurements consisted of monitoring changes in chemical shifts and line widths of the backbone amide resonances of uniformly 15N-enriched MDM2 s...More data for this Ligand-Target Pair
Affinity DataKd: 1.50E+5nMpH: 7.4 T: 2°CAssay Description:NMR measurements consisted of monitoring changes in chemical shifts and line widths of the backbone amide resonances of uniformly 15N-enriched MDM2 s...More data for this Ligand-Target Pair
Affinity DataKd: 2.44E+5nMpH: 7.4 T: 2°CAssay Description:NMR measurements consisted of monitoring changes in chemical shifts and line widths of the backbone amide resonances of uniformly 15N-enriched MDM2 s...More data for this Ligand-Target Pair
Affinity DataKd: 2.50E+5nMpH: 7.4 T: 2°CAssay Description:NMR measurements consisted of monitoring changes in chemical shifts and line widths of the backbone amide resonances of uniformly 15N-enriched MDM2 s...More data for this Ligand-Target Pair
Affinity DataKd: 2.70E+5nMpH: 7.4 T: 2°CAssay Description:NMR measurements consisted of monitoring changes in chemical shifts and line widths of the backbone amide resonances of uniformly 15N-enriched MDM2 s...More data for this Ligand-Target Pair
Affinity DataKd: 80nMpH: 7.5 T: 2°CAssay Description:Test compound was incubated for 15 minutes with 30 nM peptide fluorescein-FMDYWEGLN (Fl 9mer) and 120 nM (glycine-HDM2 17-125) in assay buffer. The p...More data for this Ligand-Target Pair
Affinity DataKd: 4.80E+3nMpH: 7.5 T: 2°CAssay Description:Test compound was incubated for 15 minutes with 30 nM peptide fluorescein-FMDYWEGLN (Fl 9mer) and 120 nM (glycine-HDM2 17-125) in assay buffer. The p...More data for this Ligand-Target Pair
Affinity DataKd: <1.00E+3nMpH: 7.4 T: 2°CAssay Description:Uniform 15N isotope labeling was achieved by expression of the protein in the M9 minimal media containing 15NH4Cl as the sole nitrogen source. The fi...More data for this Ligand-Target Pair
Affinity DataKd: 5.60E+4nMpH: 7.4 T: 2°CAssay Description:Uniform 15N isotope labeling was achieved by expression of the protein in the M9 minimal media containing 15NH4Cl as the sole nitrogen source. The fi...More data for this Ligand-Target Pair
Affinity DataKd: 5.10E+4nMpH: 7.4 T: 2°CAssay Description:Uniform 15N isotope labeling was achieved by expression of the protein in the M9 minimal media containing 15NH4Cl as the sole nitrogen source. The fi...More data for this Ligand-Target Pair
Affinity DataKd: 1.92E+5nMpH: 7.4 T: 2°CAssay Description:Uniform 15N isotope labeling was achieved by expression of the protein in the M9 minimal media containing 15NH4Cl as the sole nitrogen source. The fi...More data for this Ligand-Target Pair
Affinity DataKd: 3.65E+5nMpH: 7.4 T: 2°CAssay Description:Uniform 15N isotope labeling was achieved by expression of the protein in the M9 minimal media containing 15NH4Cl as the sole nitrogen source. The fi...More data for this Ligand-Target Pair
Affinity DataKd: 2.46E+5nMpH: 7.4 T: 2°CAssay Description:Uniform 15N isotope labeling was achieved by expression of the protein in the M9 minimal media containing 15NH4Cl as the sole nitrogen source. The fi...More data for this Ligand-Target Pair
Affinity DataKd: 1.64E+5nMpH: 7.4 T: 2°CAssay Description:Uniform 15N isotope labeling was achieved by expression of the protein in the M9 minimal media containing 15NH4Cl as the sole nitrogen source. The fi...More data for this Ligand-Target Pair
Affinity DataKd: 4.00E+4nMpH: 7.4 T: 2°CAssay Description:Uniform 15N isotope labeling was achieved by expression of the protein in the M9 minimal media containing 15NH4Cl as the sole nitrogen source. The fi...More data for this Ligand-Target Pair
Affinity DataKd: <1.00E+3nMpH: 7.4 T: 2°CAssay Description:Uniform 15N isotope labeling was achieved by expression of the protein in the M9 minimal media containing 15NH4Cl as the sole nitrogen source. The fi...More data for this Ligand-Target Pair
Affinity DataKd: 8.60E+4nMpH: 7.4 T: 2°CAssay Description:Uniform 15N isotope labeling was achieved by expression of the protein in the M9 minimal media containing 15NH4Cl as the sole nitrogen source. The fi...More data for this Ligand-Target Pair
Affinity DataKd: 1.80E+4nMpH: 7.4 T: 2°CAssay Description:Uniform 15N isotope labeling was achieved by expression of the protein in the M9 minimal media containing 15NH4Cl as the sole nitrogen source. The fi...More data for this Ligand-Target Pair
Affinity DataKd: 6.00E+4nMpH: 7.4 T: 2°CAssay Description:Uniform 15N isotope labeling was achieved by expression of the protein in the M9 minimal media containing 15NH4Cl as the sole nitrogen source. The fi...More data for this Ligand-Target Pair
Affinity DataKd: 6.80E+4nMpH: 7.4 T: 2°CAssay Description:Uniform 15N isotope labeling was achieved by expression of the protein in the M9 minimal media containing 15NH4Cl as the sole nitrogen source. The fi...More data for this Ligand-Target Pair
Affinity DataKd: 500nMpH: 7.4 T: 2°CAssay Description:MST (NanoTemper Technologies GmbH) was used to determine the binding affinities between Mdm2 (residues 1-118 T47W; 500 nM) and inhibitors. T47W mutat...More data for this Ligand-Target Pair
Affinity DataKd: 1.87E+4nMpH: 7.4 T: 2°CAssay Description:MST (NanoTemper Technologies GmbH) was used to determine the binding affinities between Mdm2 (residues 1-118 T47W; 500 nM) and inhibitors. T47W mutat...More data for this Ligand-Target Pair
Affinity DataKd: 2.77E+4nMpH: 7.4 T: 2°CAssay Description:MST (NanoTemper Technologies GmbH) was used to determine the binding affinities between Mdm2 (residues 1-118 T47W; 500 nM) and inhibitors. T47W mutat...More data for this Ligand-Target Pair
Affinity DataKd: 4.05E+4nMpH: 7.4 T: 2°CAssay Description:MST (NanoTemper Technologies GmbH) was used to determine the binding affinities between Mdm2 (residues 1-118 T47W; 500 nM) and inhibitors. T47W mutat...More data for this Ligand-Target Pair
Affinity DataKd: 3.04E+4nMpH: 7.4 T: 2°CAssay Description:MST (NanoTemper Technologies GmbH) was used to determine the binding affinities between Mdm2 (residues 1-118 T47W; 500 nM) and inhibitors. T47W mutat...More data for this Ligand-Target Pair
Affinity DataKd: 2.44E+4nMpH: 7.4 T: 2°CAssay Description:MST (NanoTemper Technologies GmbH) was used to determine the binding affinities between Mdm2 (residues 1-118 T47W; 500 nM) and inhibitors. T47W mutat...More data for this Ligand-Target Pair
Affinity DataKd: 2.10E+3nMpH: 7.4 T: 2°CAssay Description:MST (NanoTemper Technologies GmbH) was used to determine the binding affinities between Mdm2 (residues 1-118 T47W; 500 nM) and inhibitors. T47W mutat...More data for this Ligand-Target Pair
Affinity DataKd: 3.10E+3nMpH: 7.4 T: 2°CAssay Description:MST (NanoTemper Technologies GmbH) was used to determine the binding affinities between Mdm2 (residues 1-118 T47W; 500 nM) and inhibitors. T47W mutat...More data for this Ligand-Target Pair
Affinity DataKd: 1.50E+3nMpH: 7.4 T: 2°CAssay Description:MST (NanoTemper Technologies GmbH) was used to determine the binding affinities between Mdm2 (residues 1-118 T47W; 500 nM) and inhibitors. T47W mutat...More data for this Ligand-Target Pair
Affinity DataKd: 2.87E+4nMpH: 7.4 T: 2°CAssay Description:MST (NanoTemper Technologies GmbH) was used to determine the binding affinities between Mdm2 (residues 1-118 T47W; 500 nM) and inhibitors. T47W mutat...More data for this Ligand-Target Pair
Affinity DataKd: 2.60E+4nMpH: 7.4 T: 2°CAssay Description:MST (NanoTemper Technologies GmbH) was used to determine the binding affinities between Mdm2 (residues 1-118 T47W; 500 nM) and inhibitors. T47W mutat...More data for this Ligand-Target Pair
Affinity DataKd: 1.30E+3nMpH: 7.4 T: 2°CAssay Description:MST (NanoTemper Technologies GmbH) was used to determine the binding affinities between Mdm2 (residues 1-118 T47W; 500 nM) and inhibitors. T47W mutat...More data for this Ligand-Target Pair
Affinity DataKd: 7.00E+3nMpH: 7.4 T: 2°CAssay Description:MST (NanoTemper Technologies GmbH) was used to determine the binding affinities between Mdm2 (residues 1-118 T47W; 500 nM) and inhibitors. T47W mutat...More data for this Ligand-Target Pair
Affinity DataKd: 8.00E+3nMAssay Description:Binding affinity to human wild type Mdm2 by NMR ligand-protein binary titrationMore data for this Ligand-Target Pair
Affinity DataKd: 2.00E+3nMAssay Description:Binding affinity to human wild type Mdm2 by isothermal titration calorimetryMore data for this Ligand-Target Pair
Affinity DataKd: 0.0360nMAssay Description:Binding affinity to GST-thrombin-tagged human MDM2 (1 to 188) expressed in Escherichia coli after 1 to 2 mins by surface plasmon resonance spectrosco...More data for this Ligand-Target Pair
Affinity DataKd: 0.392nMAssay Description:Binding affinity to GST-thrombin-tagged human MDM2 (1 to 188) expressed in Escherichia coli after 1 to 2 mins by surface plasmon resonance spectrosco...More data for this Ligand-Target Pair
Affinity DataKd: 2.30E+5nMAssay Description:ITC experiments were run on a Malvern MicroCal Auto-ITC200 or ITC200 machine. Titration experiments were run in 20 mM Phosphate, 150 mM NaCl and 1 mM...More data for this Ligand-Target Pair
Affinity DataKd: 2.30nMAssay Description:Inhibition of human MDM2 L33E mutant (14 to 111 residues) by ITC methodMore data for this Ligand-Target Pair
Affinity DataKd: 1.00E+3nMAssay Description:ITC experiments were run on a Malvern MicroCal Auto-ITC200 or ITC200 machine. Titration experiments were run in 20 mM Phosphate, 150 mM NaCl and 1 mM...More data for this Ligand-Target Pair
Affinity DataKd: 700nMAssay Description:Binding affinity to human wild type Mdm2 by isothermal titration calorimetryMore data for this Ligand-Target Pair
Affinity DataKd: 3.40E+3nMAssay Description:Inhibition of PMDM6-F peptide binding to MDM2 binding domain (1 to 118 residues) (unknown origin) by fluorescence polarization binding assayMore data for this Ligand-Target Pair
Affinity DataKd: 0.0450nMAssay Description:Binding affinity to human MDM2 by by Surface Plasmon Resonace (SPR) spectroscopy binding assayMore data for this Ligand-Target Pair
Affinity DataKd: 1.00E+3nMAssay Description:ITC experiments were run on a Malvern MicroCal Auto-ITC200 or ITC200 machine. Titration experiments were run in 20 mM Phosphate, 150 mM NaCl and 1 mM...More data for this Ligand-Target Pair
Affinity DataKd: 0.400nMAssay Description:Binding affinity to human MDM2 by SPR analysisMore data for this Ligand-Target Pair
Affinity DataKd: 1.01E+4nMAssay Description:Inhibition of PMDM6-F peptide binding to MDM2 binding domain (1 to 118 residues) (unknown origin) by fluorescence polarization binding assayMore data for this Ligand-Target Pair
Affinity DataKd: 4.90E+3nMAssay Description:Inhibition of PMDM6-F peptide binding to MDM2 binding domain (1 to 118 residues) (unknown origin) by fluorescence polarization binding assayMore data for this Ligand-Target Pair
Affinity DataKd: 1.97E+3nMAssay Description:Inhibition of PMDM6-F peptide binding to MDM2 binding domain (1 to 118 residues) (unknown origin) by fluorescence polarization binding assayMore data for this Ligand-Target Pair
Affinity DataKd: 2.40E+4nMAssay Description:ITC experiments were run on a Malvern MicroCal Auto-ITC200 or ITC200 machine. Titration experiments were run in 20 mM Phosphate, 150 mM NaCl and 1 mM...More data for this Ligand-Target Pair
