Affinity DataKi: 16nM ΔG°: -44.0kJ/molepH: 7.4 T: 2°CAssay Description:The kinase assay uses purified recombinant enzyme and a biotinylated peptide as substrate. The biotinylated peptides were immobilized on streptavidi...More data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha(Mus musculus (mouse))
Vertex Pharmaceuticals
Vertex Pharmaceuticals
Affinity DataKi: 340nM ΔG°: -37.5kJ/molepH: 7.6 T: 2°CAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha(Mus musculus (mouse))
Vertex Pharmaceuticals
Vertex Pharmaceuticals
Affinity DataKi: 460nM ΔG°: -36.8kJ/molepH: 7.6 T: 2°CAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha(Mus musculus (mouse))
Vertex Pharmaceuticals
Vertex Pharmaceuticals
Affinity DataKi: 2.20E+3nM ΔG°: -32.8kJ/molepH: 7.6 T: 2°CAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha(Mus musculus (mouse))
Vertex Pharmaceuticals
Vertex Pharmaceuticals
Affinity DataKi: >5.00E+3nM ΔG°: >-30.8kJ/molepH: 7.6 T: 2°CAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
Affinity DataIC50: 1nMpH: 7.5 T: 2°CAssay Description:The kinase assay uses purified recombinant enzyme and a biotinylated peptide as substrate. The biotinylated peptides were immobilized on streptavidi...More data for this Ligand-Target Pair
Affinity DataIC50: 2nMpH: 7.2 T: 2°CAssay Description:In this assay, a fluorescent phosphopeptide tracer and the nonfluorescent phosphopeptides generated during a PKA reaction compete for binding to an a...More data for this Ligand-Target Pair
Affinity DataIC50: 3.60nMpH: 7.4 T: 2°CAssay Description:The kinase assay uses purified recombinant enzyme and a biotinylated peptide as substrate. The biotinylated peptides were immobilized on streptavidi...More data for this Ligand-Target Pair
Affinity DataIC50: 5nMpH: 7.2 T: 2°CAssay Description:In this assay, a fluorescent phosphopeptide tracer and the nonfluorescent phosphopeptides generated during a PKA reaction compete for binding to an a...More data for this Ligand-Target Pair
Affinity DataIC50: 5nMpH: 7.5 T: 2°CAssay Description:The kinase assay uses purified recombinant enzyme and a biotinylated peptide as substrate. The biotinylated peptides were immobilized on streptavidi...More data for this Ligand-Target Pair
Affinity DataIC50: 6nMpH: 7.5 T: 2°CAssay Description:The kinase assay uses purified recombinant enzyme and a biotinylated peptide as substrate. The biotinylated peptides were immobilized on streptavidi...More data for this Ligand-Target Pair
Affinity DataIC50: 9nMpH: 7.5 T: 2°CAssay Description:The kinase assay uses purified recombinant enzyme and a biotinylated peptide as substrate. The biotinylated peptides were immobilized on streptavidi...More data for this Ligand-Target Pair
Affinity DataIC50: 15nMpH: 7.2 T: 2°CAssay Description:The recombinant alpha catalytic subunit of bovine PKA was assayed with a peptide substrate and test compound in the presence of 40 uM ATP/ [gamma-33P...More data for this Ligand-Target Pair
Affinity DataIC50: 16nMpH: 7.5 T: 2°CAssay Description:The kinase assay uses purified recombinant enzyme and a biotinylated peptide as substrate. The biotinylated peptides were immobilized on streptavidi...More data for this Ligand-Target Pair
Affinity DataIC50: 27nMpH: 7.2 T: 2°CAssay Description:The recombinant alpha catalytic subunit of bovine PKA was assayed with a peptide substrate and test compound in the presence of 40 uM ATP/ [gamma-33P...More data for this Ligand-Target Pair
Affinity DataIC50: 30nMpH: 7.2 T: 2°CAssay Description:In this assay, a fluorescent phosphopeptide tracer and the nonfluorescent phosphopeptides generated during a PKA reaction compete for binding to an a...More data for this Ligand-Target Pair
Affinity DataIC50: 30nMpH: 7.5 T: 2°CAssay Description:The activity of PKA, activated by cAMP, is measured by its ability to transfer phosphate from [gamma-32P]ATP to histone.More data for this Ligand-Target Pair
Affinity DataIC50: 33nMpH: 7.2 T: 2°CAssay Description:The recombinant alpha catalytic subunit of bovine PKA was assayed with a peptide substrate and test compound in the presence of 40 uM ATP/ [gamma-33P...More data for this Ligand-Target Pair
Affinity DataIC50: 38nMpH: 7.4 T: 2°CAssay Description:The kinase assay uses purified recombinant enzyme and a biotinylated peptide as substrate. The biotinylated peptides were immobilized on streptavidi...More data for this Ligand-Target Pair
Affinity DataIC50: 39nMpH: 7.2 T: 2°CAssay Description:In this assay, a fluorescent phosphopeptide tracer and the nonfluorescent phosphopeptides generated during a PKA reaction compete for binding to an a...More data for this Ligand-Target Pair
Affinity DataIC50: 40nMpH: 7.4 T: 2°CAssay Description:IC50 is the inhibitor concentration which inhibits 50% of kinase activity that catalyzes the transfer of the terminal phosphate from [gamma-32P] labe...More data for this Ligand-Target Pair
Affinity DataIC50: <45nMpH: 6.8 T: 2°CAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
Affinity DataIC50: 56nMpH: 6.8 T: 2°CAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
Affinity DataIC50: 60nMpH: 7.5 T: 2°CAssay Description:The activity of PKA, activated by cAMP, is measured by its ability to transfer phosphate from [gamma-32P]ATP to histone.More data for this Ligand-Target Pair
Affinity DataIC50: 73nMpH: 7.2 T: 2°CAssay Description:The recombinant alpha catalytic subunit of bovine PKA was assayed with a peptide substrate and test compound in the presence of 40 uM ATP/ [gamma-33P...More data for this Ligand-Target Pair
Affinity DataIC50: 100nMpH: 7.4 T: 2°CAssay Description:IC50 is the inhibitor concentration which inhibits 50% of kinase activity that catalyzes the transfer of the terminal phosphate from [gamma-32P] labe...More data for this Ligand-Target Pair
Affinity DataIC50: 100nMpH: 7.4 T: 2°CAssay Description:IC50 is the inhibitor concentration which inhibits 50% of kinase activity that catalyzes the transfer of the terminal phosphate from [gamma-32P] labe...More data for this Ligand-Target Pair
Affinity DataIC50: 121nMpH: 8.5 T: 2°CAssay Description:The activity of PKA, activated by cAMP, is measured by its ability to transfer phosphate from [gamma-32P]ATP to histone.More data for this Ligand-Target Pair
Affinity DataIC50: 170nMpH: 7.2 T: 2°CAssay Description:The recombinant alpha catalytic subunit of bovine PKA was assayed with a peptide substrate and test compound in the presence of 40 uM ATP/ [gamma-33P...More data for this Ligand-Target Pair
Affinity DataIC50: 170nMpH: 7.2 T: 2°CAssay Description:The recombinant alpha catalytic subunit of bovine PKA was assayed with a peptide substrate and test compound in the presence of 40 uM ATP/ [gamma-33P...More data for this Ligand-Target Pair
Affinity DataIC50: 170nMpH: 7.2 T: 2°CAssay Description:The recombinant alpha catalytic subunit of bovine PKA was assayed with a peptide substrate and test compound in the presence of 40 uM ATP/ [gamma-33P...More data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha(Homo sapiens (Human))
The Scripps Research Institute
The Scripps Research Institute
Affinity DataIC50: 186nMpH: 7.3 T: 2°CAssay Description:Reaction mixture of kemptide, ATP, and test compound was added to the wells using a BioRAPTR FRD Workstation (Aurora Discovery). Reaction was started...More data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha(Homo sapiens (Human))
The Scripps Research Institute
The Scripps Research Institute
Affinity DataIC50: 190nMpH: 7.5 T: 2°CAssay Description:Kinase activity was assayed in reaction buffer containing substrate peptide, enzyme, and inhibitor compound in the presence ATP/[gamma-33P] ATP. 33P ...More data for this Ligand-Target Pair
Affinity DataIC50: 200nMpH: 7.4 T: 2°CAssay Description:IC50 is the inhibitor concentration which inhibits 50% of kinase activity that catalyzes the transfer of the terminal phosphate from [gamma-32P] labe...More data for this Ligand-Target Pair
Affinity DataIC50: 360nMpH: 7.2 T: 2°CAssay Description:In this assay, a fluorescent phosphopeptide tracer and the nonfluorescent phosphopeptides generated during a PKA reaction compete for binding to an a...More data for this Ligand-Target Pair
Affinity DataIC50: 394nMpH: 6.8 T: 2°CAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair
Affinity DataIC50: 400nMpH: 7.2 T: 2°CAssay Description:In this assay, a fluorescent phosphopeptide tracer and the nonfluorescent phosphopeptides generated during a PKA reaction compete for binding to an a...More data for this Ligand-Target Pair
Affinity DataIC50: 410nMpH: 7.2 T: 2°CAssay Description:The recombinant alpha catalytic subunit of bovine PKA was assayed with a peptide substrate and test compound in the presence of 40 uM ATP/ [gamma-33P...More data for this Ligand-Target Pair
Affinity DataIC50: 440nMpH: 7.5 T: 2°CAssay Description:The activity of PKA, activated by cAMP, is measured by its ability to transfer phosphate from [gamma-32P]ATP to histone.More data for this Ligand-Target Pair
Affinity DataIC50: 440nMpH: 7.5 T: 2°CAssay Description:The activity of PKA, activated by cAMP, is measured by its ability to transfer phosphate from [gamma-32P]ATP to histone.More data for this Ligand-Target Pair
Affinity DataIC50: 600nMpH: 7.4 T: 2°CAssay Description:IC50 is the inhibitor concentration, which inhibits 50% of PKA activity that catalyzes the transfer of the terminal phosphate from [gamma-32P] label...More data for this Ligand-Target Pair
Affinity DataIC50: 610nMpH: 7.2 T: 2°CAssay Description:The recombinant alpha catalytic subunit of bovine PKA was assayed with a peptide substrate and test compound in the presence of 40 uM ATP/ [gamma-33P...More data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha(Homo sapiens (Human))
The Scripps Research Institute
The Scripps Research Institute
Affinity DataIC50: 637nMpH: 7.3 T: 2°CAssay Description:Reaction mixture of kemptide, ATP, and test compound was added to the wells using a BioRAPTR FRD Workstation (Aurora Discovery). Reaction was started...More data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha(Homo sapiens (Human))
The Scripps Research Institute
The Scripps Research Institute
Affinity DataIC50: 692nMpH: 7.3 T: 2°CAssay Description:Reaction mixture of kemptide, ATP, and test compound was added to the wells using a BioRAPTR FRD Workstation (Aurora Discovery). Reaction was started...More data for this Ligand-Target Pair
Affinity DataIC50: 800nMpH: 7.2 T: 2°CAssay Description:In this assay, a fluorescent phosphopeptide tracer and the nonfluorescent phosphopeptides generated during a PKA reaction compete for binding to an a...More data for this Ligand-Target Pair
TargetcAMP-dependent protein kinase catalytic subunit alpha(Homo sapiens (Human))
The Scripps Research Institute
The Scripps Research Institute
Affinity DataIC50: 940nMpH: 7.3 T: 2°CAssay Description:Reaction mixture of kemptide, ATP, and test compound was added to the wells using a BioRAPTR FRD Workstation (Aurora Discovery). Reaction was started...More data for this Ligand-Target Pair
Affinity DataIC50: 1.00E+3nMpH: 7.5 T: 2°CAssay Description:The activity of PKA, activated by cAMP, is measured by its ability to transfer phosphate from [gamma-32P]ATP to histone.More data for this Ligand-Target Pair
Affinity DataIC50: 1.00E+3nMpH: 7.2 T: 2°CAssay Description:The recombinant alpha catalytic subunit of bovine PKA was assayed with a peptide substrate and test compound in the presence of 40 uM ATP/ [gamma-33P...More data for this Ligand-Target Pair
Affinity DataIC50: 1.10E+3nMpH: 8.5 T: 2°CAssay Description:The activity of PKA, activated by cAMP, is measured by its ability to transfer phosphate from [gamma-32P]ATP to histone.More data for this Ligand-Target Pair
Affinity DataIC50: 1.49E+3nMpH: 6.8 T: 2°CAssay Description:The enzyme activity was assayed by using an ATP regenerative NADH consuming system. The reaction was started with adding ATP to the mixture containin...More data for this Ligand-Target Pair