Solution Information | help | |
Enzyme: | DNA topoisomerase 4 subunit A/B | |
inhibitor: | BDBM24601 | |
substrate: | BDBM2 | |
Solution Type: | Aqueous | |
pH at Preparation: | 7.5 | |
Temp. Prep.: | 30 | |
Comments: | n/a | |
Solute 1: | PEP | |
Concentration: | 2.5 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 2: | KCl | |
Concentration: | 150 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 3: | MgCl2 | |
Concentration: | 1.5 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 4: | ATP | |
Concentration: | 0.55 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 5: | NADH | |
Concentration: | 0.2 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 6: | pyruvate kinase | |
Concentration: | 0.03 mg/mL | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solvent 1: | water | |
Concentration: | 1.0 | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solvent 2: | DMSO | |
Concentration: | 4% | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
pH Buffer 1: | Tris-HCl | |
Concentration: | 100 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
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