Solution Information | help | |
Enzyme: | Glycogen synthase kinase-3 beta | |
inhibitor: | BDBM35659 | |
substrate: | GSK-3 Substrate Peptide | |
Solution Type: | Aqueous | |
pH at Preparation: | 7.5 | |
Temp. Prep.: | 30 | |
Comments: | n/a | |
Solute 1: | ATP | |
Concentration: | 65 uM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 2: | NADH | |
Concentration: | 200 uM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 3: | pyruvate kinase/lactate dehydrogenase | |
Concentration: | 150 ug/mL, 50 ugmL | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 4: | phosphoenolpyruvate | |
Concentration: | 2.5 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solvent 1: | water | |
Concentration: | 1.0 | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solvent 2: | DMSO | |
Concentration: | 2.5% | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
pH Buffer 1: | HEPES | |
Concentration: | 0.1 M | |
ΔHion: | n/a | |
Comments: | n/a | |
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