Reaction Details Report a problem with these data
Cell Reactant:
Thymidine Kinase
Syringe Reactant:
BDBM1
Meas. Tech.:
ITC
Entry Date.:
12/13/00
ΔG°:
-7.23±0.35 (kcal/mole)
pH:
7.5±n/a
Temperature:
298.15±n/a (K)
ΔH° :
-19.12±0.96 (kJ/mole)
Corrected for ΔHioniz:
yes
Protons Released:
0
ΔCp :
-0.36±0.02 (kJ/mole)
ΔS° :
-0.04±0.01 (kJ/mole-K)
Citation
Perozzo, R; Jelesarov, I; Bosshard, HR; Folkers, G; Scapozza, L Compulsory order of substrate binding to herpes simplex virus type 1 thymidine kinase. A calorimetric study. J Biol Chem 275:16139-45 (2000) [PubMed] Article
Cell React
Source:
Expressed as glutathione S-transferase fusion protein in E. coli.
Prep. Method:
Purified by glutathione affinity chromatography.
Name:
Thymidine kinase
Synonyms:
KITH_HHV11 | TK
Type:
Enzyme
Mol. Mass.:
40978.57
Organism:
HHV-1
Description:
P03176
Residue:
376
Sequence:
MASYPCHQHASAFDQAARSRGHNNRRTALRPRRQQEATEVRPEQKMPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN