Reaction Details  Report a problem with these data
Cell Reactant:
D44.1 Fab
Syringe Reactant:
Lysozyme (hen egg)
Meas. Tech.:
ITC
Entry Date.:
12/11/00
ΔG°:
-40.5±0.4 (kJ/mole)
pH:
7.1±n/a
Log10Kb:
6.3
Temperature:
297.35±0.1 (K)
ΔH° :
-43.3±n/a (kJ/mole)
ΔHobs :
-43.3±n/a (kJ/mole)
ΔCp :
-1.16±0.006 (kJ/mole)
ΔS° :
-0.009±0.006 (kJ/mole-K)
Citation
 Schwarz, FP; Tello, D; Goldbaum, FA; Mariuzza, RA; Poljak, RJ Thermodynamics of antigen-antibody binding using specific anti-lysozyme antibodies. Eur J Biochem 228:388-94 (1995) [PubMed]
Cell React
Prep. Method:
Papain-cleaved antigen binding fragment of D44.1
Name:
D44.1 Fab
Synonyms:
n/a
Type:
Protein Complex
Mol. Mass.:
n/a
Description:
n/a
Components:
This complex has 2 components.
Component 1
Name:
D44.1 Fab chain A
Synonyms:
n/a
Type:
Enzyme Subunit
Mol. Mass.:
23585.62
Organism:
Mus musculus (mouse)
Description:
Immunoglobulin
Residue:
214
Sequence:
DIELTQSPATLSVTPGDSVSLSCRASQSISNNLHWYQQKSHESPRLLIKYVSQSSSGIPSRFSGSGSGTDFTLSINSVETEDFGMYFCQQSNSWPRTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC
  
Component 2
Name:
D44.1 Fab chain B
Synonyms:
n/a
Type:
Enzyme Subunit
Mol. Mass.:
23199.17
Organism:
Mus musculus (mouse)
Description:
Immunoglobulin
Residue:
218
Sequence:
QVQLQESGAEVMKPGASVKISCKATGYTFSTYWIEWVKQRPGHGLEWIGEILPGSGSTYYNEKFKGKATFTADTSSNTAYMQLSSLTSEDSAVYYCARGDGNYGYWGQGTTLTVSSASTTPPSVFPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSPRPSETVTCNVAHPASSTKVDKKIVPRDC
  
Syringe React
Source:
Sigma Chemical Co.
Name:
Lysozyme (hen egg)
Synonyms:
D1.3 Fv | D1.3 mAb | LYZ1_MOUSE | Lyz1 | Lzp-s | hen egg lysozyme (HEL) | hen egg lysozyme(HEL)
Type:
Enzyme
Mol. Mass.:
16805.63
Organism:
Mus musculus (mouse)
Description:
P17897
Residue:
148
Sequence:
MKALLTLGLLLLSVTAQAKVYNRCELARILKRNGMDGYRGVKLADWVCLAQHESNYNTRATNYNRGDRSTDYGIFQINSRYWCNDGKTPRSKNACGINCSALLQDDITAAIQCAKRVVRDPQGIRAWVAWRTQCQNRDLSQYIRNCGV