Reaction Details  Report a problem with these data
Cell Reactant:
D1.3 Fv
Syringe Reactant:
Lysozyme (hen egg)
Meas. Tech.:
ITC
Entry Date.:
12/11/00
ΔG°:
-42.6±1 (kJ/mole)
pH:
7.1±n/a
Log10Kb:
6.5
Temperature:
322.95±0.1 (K)
ΔH° :
-129±n/a (kJ/mole)
ΔHobs :
-129±n/a (kJ/mole)
ΔCp :
-1.58±0.06 (kJ/mole)
ΔS° :
-0.268±0.022 (kJ/mole-K)
Citation
 Schwarz, FP; Tello, D; Goldbaum, FA; Mariuzza, RA; Poljak, RJ Thermodynamics of antigen-antibody binding using specific anti-lysozyme antibodies. Eur J Biochem 228:388-94 (1995) [PubMed]
Cell React
Source:
Recombinant protein expressed from E. coli
Prep. Method:
Cell lysate was purified by affinity chromatography
Name:
D1.3 Fv
Synonyms:
n/a
Type:
Antibody
Mol. Mass.:
24477.57
Organism:
Mus musculus (mouse)
Description:
Fv fragment of mouse monoclonal antobody D1.3
Residue:
222
Sequence:
DIVLTQSPASLSASVGETVTITCRASGNIHNYLAWYQQKQGKSPQLLVYYTLADGVPSRFSGSGSGTQYSLKINSLQPEDFGSYYCQHFWSTPRTFGGGTKLEIKRQVQLQESGPGLVAPSQSLSITCTVSGFSLTGYGVNWVRQPPGKGLEWLGMIWGDGNTDYNSALKSRLSISKDNSKSQVFLKMNSLHTDDTARYYCARERDYRLDYWGQGTTLTVSS
  
Syringe React
Source:
Sigma Chemical Co.
Name:
Lysozyme (hen egg)
Synonyms:
D1.3 Fv | D1.3 mAb | hen egg lysozyme (HEL) | hen egg lysozyme(HEL)
Type:
Enzyme
Mol. Mass.:
16805.63
Organism:
Mus musculus (mouse)
Description:
P17897
Residue:
148
Sequence:
MKALLTLGLLLLSVTAQAKVYNRCELARILKRNGMDGYRGVKLADWVCLAQHESNYNTRATNYNRGDRSTDYGIFQINSRYWCNDGKTPRSKNACGINCSALLQDDITAAIQCAKRVVRDPQGIRAWVAWRTQCQNRDLSQYIRNCGV