Cell Reactant:
Heat Shock Protein 90 (Hsp90)
Syringe Reactant:
BDBM31996
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
09/29/09
ΔG°:
-7.63±n/a (kcal/mole)
pH:
8±n/a
Log10Kb:
4.3
Temperature:
288.15±n/a (K)
ΔHobs :
-4.88±0.02 (kJ/mole)
Corrected for ΔHioniz:
no
ΔS° :
0.01±n/a (kJ/mole-K)
Citation
 Nilapwar, SWilliams, EFu, CProdromou, CPearl, LHWilliams, MALadbury, JE Structural-thermodynamic relationships of interactions in the N-terminal ATP-binding domain of Hsp90. J Mol Biol 392:923-36 (2009) [PubMed]  Article
Cell React
Source:
His-tagged N-terminal domain of yeast HSP90 was expressed and purified.
Prep. Method:
Protein was purified by using nickel affinity column, and was subsequently concentrated by an (NH4)2SO4 precipitation and purified further using gel filtration chromatography.
Name:
Heat Shock Protein 90 (Hsp90)
Synonyms:
82 kDa heat shock protein | ATP-dependent molecular chaperone HSP82 | Heat shock protein Hsp90 heat-inducible isoform
Type:
N-terminal domain
Mol. Mass.:
23389.88
Organism:
Saccharomyces cerevisiae (Baker s yeast)
Description:
P02829 aa 1-207
Residue:
207
Sequence:
MASETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSLSDPKQLETEPDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSGTKAFMEALSAGADVSMIGQFGVGFYSLFLVADRVQVISKSNDDEQYIWESNAGGSFTVTLDEVNERIGRGTILRLFLKDDQLEYLEEKRIKEVIKRHSEFVAYPIQL
  
Syringe React
Name:
BDBM31996
Synonyms:
17-(dimethylaminoethylamino)-17-demethoxygeldanamycin | 17-DMAG | CHEMBL383824
Type:
n/a
Emp. Form.:
C32H48N4O8
Mol. Mass.:
616.7455
SMILES:
CO[C@H]1C[C@H](C)Cc2c(O)c(NC(=O)C(C)=CC=C[C@H](OC)[C@@H](OC(N)=O)\C(C)=C\[C@H](C)[C@H]1O)cc(O)c2N=CCN(C)C |r,w:16.16,38.39,t:28|
Structure:
Search PDB for entries with ligand similarity: