Reaction Details Report a problem with these data
Target
Caspase-3
Ligand
BDBM10297
Substrate
Caspase Fluorogenic Substrate
Meas. Tech.
Caspase Inhibition Assay
pH
7.5±n/a
Temperature
303.15±n/a K
Ki
500±n/a nM
IC50
1000±n/a nM
Citation
 Lee, D; Long, SA; Murray, JH; Adams, JL; Nuttall, ME; Nadeau, DP; Kikly, K; Winkler, JD; Sung, CM; Ryan, MD; Levy, MA; Keller, PM; DeWolf, WE Potent and selective nonpeptide inhibitors of caspases 3 and 7. J Med Chem 44:2015-26 (2001) [PubMed]  Article 
Target
Name:
Caspase-3
Synonyms:
Apopain | CASP-3 | CASP3 | CASP3_HUMAN | CPP-32 | CPP32 | Caspase 3 | Caspase-3 subunit p12 | Caspase-3 subunit p17 | Cysteine protease CPP32 | SCA-1 | SREBP cleavage activity 1 | Yama protein | caspase-3 preproprotein
Type:
Hydrolase; heterotetramer of two heterodimers
Mol. Mass.:
31607.55
Organism:
Human
Description:
n/a
Residue:
277
Sequence:
MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDNSYKMDYPEMGLCIIINNKNFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFIIQACRGTELDCGIETDSGVDDDMACHKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQSLCAMLKQYADKLEFMHILTRVNRKVATEFESFSFDATFHAKKQIPCIVSMLTKELYFYH
  
Inhibitor
Name:
BDBM10297
Synonyms:
1-methyl-5-nitro-2,3-dihydro-1H-indole-2,3-dione | Isatin analog 1
Type:
Small organic molecule
Emp. Form.:
C9H6N2O4
Mol. Mass.:
206.1549
SMILES:
CN1C(=O)C(=O)c2cc(ccc12)N(=O)=O
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
Caspase Fluorogenic Substrate
Synonyms:
Ac-DEVD-AMC | Caspase Fluorogenic Substrate
Type:
Peptide
Mol. Mass.:
1461.77
Organism:
n/a
Description:
AMC=7-Amino-4-methylcoumarin
Residue:
16
Sequence:
ACASPGLVALASPAMC