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Target
Replicase polyprotein 1ab [1541-1855]
Ligand
BDBM154573
Substrate
n/a
Meas. Tech.
Fluorimetric Assay
pH
7.5±n/a
Ki
1.15e+4±n/a nM
IC50
1.09e+4± 9e+2 nM
Comments
extracted
Citation
 Lee, H; Lei, H; Santarsiero, BD; Gatuz, JL; Cao, S; Rice, AJ; Patel, K; Szypulinski, MZ; Ojeda, I; Ghosh, AK; Johnson, ME Inhibitor recognition specificity of MERS-CoV papain-like protease may differ from that of SARS-CoV. ACS Chem Biol 10:1456-65 (2015) [PubMed]  Article  BDB Entry
Target
Name:
Replicase polyprotein 1ab [1541-1855]
Synonyms:
SARS Coronavirus Coronavirus Papain-Like Protease | SARS-CoV PLpro | SARS-CoV papain-like protease (SARS-PLpro)
Type:
Hydrolase
Mol. Mass.:
35352.30
Organism:
Human SARS coronavirus (SARS-CoV)
Description:
The SARS-CoV PLpro enzyme (residues 1541 -1855) was expressed and purified from E. coli.
Residue:
315
Sequence:
EVKTIKVFTTVDNTNLHTQLVDMSMTYGQQFGPTYLDGADVTKIKPHVNHEGKTFFVLPSDDTLRSEAFEYYHTLDESFLGRYMSALNHTKKWKFPQVGGLTSIKWADNNCYLSSVLLALQQLEVKFNAPALQEAYYRARAGDAANFCALILAYSNKTVGELGDVRETMTHLLQHANLESAKRVLNVVCKHCGQKTTTLTGVEAVMYMGTLSYDNLKTGVSIPCVCGRDATQYLVQQESSFVMMSAPPAEYKLQQGTFLCANEYTGNYQCGHYTHITAKETLYRIDGAHLTKMSEYKGPVTDVFYKETSYTTTIK
  
Inhibitor
Name:
BDBM154573
Synonyms:
8-(trifluoromethyl)-9H-purin-6-amine (4) | acs.jmedchem.1c00409_ST.555 | jm5b01461, Compound 188
Type:
Small organic molecule
Emp. Form.:
C6H4F3N5
Mol. Mass.:
203.1247
SMILES:
Nc1ncnc2[nH]c(nc12)C(F)(F)F
Structure:
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