Reaction Details Report a problem with these data
Target
Replicase polyprotein 1ab [1541-1855]
Ligand
BDBM154574
Substrate
n/a
Meas. Tech.
Fluorimetric Assay
pH
7.5±n/a
IC50
2.26e+3± 9e+1 nM
Comments
extracted
Citation
 Lee, H; Lei, H; Santarsiero, BD; Gatuz, JL; Cao, S; Rice, AJ; Patel, K; Szypulinski, MZ; Ojeda, I; Ghosh, AK; Johnson, ME Inhibitor recognition specificity of MERS-CoV papain-like protease may differ from that of SARS-CoV. ACS Chem Biol 10:1456-65 (2015) [PubMed]  Article 
Target
Name:
Replicase polyprotein 1ab [1541-1855]
Synonyms:
R1AB_SARS | SARS Coronavirus Coronavirus Papain-Like Protease | SARS-CoV PLpro | SARS-CoV papain-like protease (SARS-PLpro) | rep
Type:
Hydrolase
Mol. Mass.:
35352.30
Organism:
Human SARS coronavirus (SARS-CoV)
Description:
The SARS-CoV PLpro enzyme (residues 1541 -1855) was expressed and purified from E. coli.
Residue:
315
Sequence:
EVKTIKVFTTVDNTNLHTQLVDMSMTYGQQFGPTYLDGADVTKIKPHVNHEGKTFFVLPSDDTLRSEAFEYYHTLDESFLGRYMSALNHTKKWKFPQVGGLTSIKWADNNCYLSSVLLALQQLEVKFNAPALQEAYYRARAGDAANFCALILAYSNKTVGELGDVRETMTHLLQHANLESAKRVLNVVCKHCGQKTTTLTGVEAVMYMGTLSYDNLKTGVSIPCVCGRDATQYLVQQESSFVMMSAPPAEYKLQQGTFLCANEYTGNYQCGHYTHITAKETLYRIDGAHLTKMSEYKGPVTDVFYKETSYTTTIK
  
Inhibitor
Name:
BDBM154574
Synonyms:
N-(2H-1,3-benzodioxol-5-ylmethyl)-1-[(1S)-1-(naphthalen-1-yl)ethyl]piperidine-4-carboxamide (I-4) | med.21724, Compound 166
Type:
Small organic molecule
Emp. Form.:
C26H28N2O3
Mol. Mass.:
416.5121
SMILES:
C[C@H](N1CCC(CC1)C(=O)NCc1ccc2OCOc2c1)c1cccc2ccccc12 |r|
Structure:
Search PDB for entries with ligand similarity: