Affinity DataKi: 32.4nMpH: 7.5Assay Description:Assay buffer was 20 mM HEPES, pH 7.5, 10 mM MgCl2, 0.01% BSA, 1 mM DTT, 0.0005% Tween 20, and 2% DMSO. Inactivation kinetic reactions were performed ...More data for this Ligand-Target Pair
Affinity DataKi: 170nMAssay Description:Inhibition of BLK (unknown origin) using KVEKIGEGTYGVVYK as substrate in presence of [gamma33P]ATP as substrate in presence of [gamma33P]ATP by scint...More data for this Ligand-Target Pair
Affinity DataKi: 190nMAssay Description:Inhibition of BLK (unknown origin) using KVEKIGEGTYGVVYK as substrate in presence of [gamma33P]ATP as substrate in presence of [gamma33P]ATP by scint...More data for this Ligand-Target Pair
Affinity DataKi: >1.40E+3nMAssay Description:Inhibition of recombinant BLK after 1 hr by scintillation counter analysis in presence of gamma-[33P]ATPMore data for this Ligand-Target Pair
Affinity DataKi: >1.00E+4nMAssay Description:Inhibition of Blk (unknown origin)More data for this Ligand-Target Pair
Affinity DataKi: >1.00E+4nMAssay Description:Inhibition of Blk (unknown origin)More data for this Ligand-Target Pair
Affinity DataKi: 3.24E+4nMAssay Description:Inhibition of full length recombinant human His-tagged cytoplasmic BLK expressed in baculovirus expression system by KT236 probe based TR-FRET assayMore data for this Ligand-Target Pair
Affinity DataIC50: 0.100nMAssay Description:Inhibition of BLK (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 0.100nMAssay Description:Inhibition of recombinant full length human His-tagged BLK cytoplasmic domain expressed in baculovirus expression system using tyrosine-1 peptide as ...More data for this Ligand-Target Pair
Affinity DataIC50: 0.200nMAssay Description:IC50s were determined using the in vitro HotSpot kinase assay (purified enzymes, 33P-ATP, an appropriate substrate and 1 uM ATP.). Reaction condition...More data for this Ligand-Target Pair
Affinity DataIC50: 0.200nMAssay Description:Inhibition of recombinant human BLK using Poly(Glu, Tyr) 4:1 as substrate after 1 hr by ELISAMore data for this Ligand-Target Pair
Affinity DataIC50: 0.200nMAssay Description:IC50s were determined using the in vitro HotSpot kinase assay (purified enzymes, 33P-ATP, an appropriate substrate and 1 μM ATP.). For enzyme in...More data for this Ligand-Target Pair
Affinity DataIC50: 0.230nMAssay Description:Inhibition of human BLK using poly[Glu:Tyr] (4:1) as substrate preincubated for 60 mins followed by [gamma-33P]-ATP addition and measured after 120 m...More data for this Ligand-Target Pair
Affinity DataIC50: 0.230nMAssay Description:Inhibition of human BLK using poly[Glu:Tyr] (4:1) as substrate preincubated for 60 mins followed by [gamma-33P]-ATP addition and measured after 120 m...More data for this Ligand-Target Pair
Affinity DataIC50: 0.5nMAssay Description:IC50s were determined using the in vitro HotSpot kinase assay (purified enzymes, 33P-ATP, an appropriate substrate and 1 uM ATP.). Reaction condition...More data for this Ligand-Target Pair
Affinity DataIC50: 0.5nMAssay Description:Inhibition of recombinant human BLK using Poly(Glu, Tyr) 4:1 as substrate after 1 hr by ELISAMore data for this Ligand-Target Pair
Affinity DataIC50: 0.5nMAssay Description:Inhibition of BLK (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 0.5nMAssay Description:IC50s were determined using the in vitro HotSpot kinase assay (purified enzymes, 33P-ATP, an appropriate substrate and 1 μM ATP.). For enzyme in...More data for this Ligand-Target Pair
Affinity DataIC50: 0.5nMAssay Description:Inhibition of BLK (unknown origin) using peptide substrate in presence of ATP by caliper electrophoresis methodMore data for this Ligand-Target Pair
Affinity DataIC50: <0.508nMAssay Description:Table 3 and 4: The HotSpot kinase profiling and screening assays were carried out using the method of Anastassiadis et al., Nat. Biotechnol. (2011) V...More data for this Ligand-Target Pair
Affinity DataIC50: <0.508nMAssay Description:Table 3 and 4: The HotSpot kinase profiling and screening assays were carried out using the method of Anastassiadis et al., Nat. Biotechnol. (2011) V...More data for this Ligand-Target Pair
Affinity DataIC50: <0.508nMAssay Description:Table 3 and 4: The HotSpot kinase profiling and screening assays were carried out using the method of Anastassiadis et al., Nat. Biotechnol. (2011) V...More data for this Ligand-Target Pair
Affinity DataIC50: <0.508nMAssay Description:Table 3 and 4: The HotSpot kinase profiling and screening assays were carried out using the method of Anastassiadis et al., Nat. Biotechnol. (2011) V...More data for this Ligand-Target Pair
Affinity DataIC50: <0.508nMAssay Description:Table 3 and 4: The HotSpot kinase profiling and screening assays were carried out using the method of Anastassiadis et al., Nat. Biotechnol. (2011) V...More data for this Ligand-Target Pair
Affinity DataIC50: <0.508nMAssay Description:Table 3 and 4: The HotSpot kinase profiling and screening assays were carried out using the method of Anastassiadis et al., Nat. Biotechnol. (2011) V...More data for this Ligand-Target Pair
Affinity DataIC50: 0.580nMAssay Description:Preparation of Compounds to be Tested:1) Using DMSO to prepare 50× compound stock solutions (same as the stock solution in Example 34) for later use;...More data for this Ligand-Target Pair
Affinity DataIC50: 0.700nMAssay Description:Inhibition of BLK (unknown origin) using STK as substrate by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 1.10nMAssay Description:Inhibition of human BLK using poly[Glu:Tyr] (4:1) as substrate preincubated for 60 mins followed by [gamma-33P]-ATP addition and measured after 120 m...More data for this Ligand-Target Pair
Affinity DataIC50: 1.10nMAssay Description:Inhibition of BLK (unknown origin) by ADP-Glo assayMore data for this Ligand-Target Pair
Affinity DataIC50: 1.10nMAssay Description:Inhibition of human BLK using poly[Glu:Tyr](4:1) as substrate by [gamma-33P]-ATP assayMore data for this Ligand-Target Pair
Affinity DataIC50: 1.10nMAssay Description:Inhibition of human BLK using poly[Glu:Tyr] (4:1) as substrate preincubated for 60 mins followed by [gamma-33P]-ATP addition and measured after 120 m...More data for this Ligand-Target Pair
Affinity DataIC50: 1.10nMAssay Description:Inhibition of human BLK using poly[Glu:Tyr](4:1) as substrate by [gamma-33P]-ATP assayMore data for this Ligand-Target Pair
Affinity DataIC50: 1.60nMAssay Description:Preparation of Compounds to be Tested:1) Using DMSO to prepare 50× compound stock solutions (same as the stock solution in Example 34) for later use;...More data for this Ligand-Target Pair
Affinity DataIC50: 2.20nMAssay Description:Inhibition of BLK (unknown origin) using peptide substrate in presence of ATP by caliper electrophoresis methodMore data for this Ligand-Target Pair
Affinity DataIC50: 2.5nMAssay Description:Inhibition of BLK (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 2.70nMAssay Description:Inhibition of Blk (unknown origin) after 1 hr by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 4.20nMAssay Description:Inhibition of Blk (unknown origin) after 1 hr by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 5nMAssay Description:Inhibition of BLK (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: <5nMAssay Description:IC50 Table 13-20: The protocol calls for test compound of the invention to be incubated with kinase, substrate, cofactors, and radio-isotope-labeled ...More data for this Ligand-Target Pair
Affinity DataIC50: <5nMAssay Description:IC50 Table 13-20: The protocol calls for test compound of the invention to be incubated with kinase, substrate, cofactors, and radio-isotope-labeled ...More data for this Ligand-Target Pair
Affinity DataIC50: 5.40nMAssay Description:Preparation of Compounds to be Tested:1) Using DMSO to prepare 50× compound stock solutions (same as the stock solution in Example 34) for later use;...More data for this Ligand-Target Pair
Affinity DataIC50: 5.5nMAssay Description:Inhibition of (S)-3-(3-(5-acrylamido-6-(3-(5-(2-methyl-5-(3-(trifluoromethyl)benzamido)benzamido)pyrimidin-2-ylamino)phenylamino)-6-oxohexylamino)-3-...More data for this Ligand-Target Pair
Affinity DataIC50: 5.60nMT: 2°CAssay Description:In vitro enzyme activity assay: wild-type and various mutants (T790M, L858R, L861Q, L858 R/T790M) EGFR, Z′-Lyte Kinase Assay Kit were purchased...More data for this Ligand-Target Pair
Affinity DataIC50: 5.90nMAssay Description:Inhibition of wild type N-terminal GST tagged BLK (unknown origin) (1 to 505 residues) expressed in sf21 insect cell using TK as substrate incubated ...More data for this Ligand-Target Pair
Affinity DataIC50: 6.10nMAssay Description:Inhibition of wild type N-terminal GST tagged BLK (unknown origin) (1 to 505 residues) expressed in sf21 insect cell using TK as substrate incubated ...More data for this Ligand-Target Pair
Affinity DataIC50: 6.30nMAssay Description:Inhibition of BLK (unknown origin) using peptide substrate in presence of ATPMore data for this Ligand-Target Pair
