Reaction Details  Report a problem with these data
Cell Reactant:
HyHEL-5 Fab
Syringe Reactant:
Lysozyme (bobwhite quail)
Meas. Tech.:
ITC and sedimentation equilibrium
Entry Date.:
12/13/00
ΔG°:
-39.06±0.42 (kJ/mole)
pH:
8±n/a
Temperature:
298.15±n/a (K)
ΔH° :
-76.86±3.36 (kJ/mole)
ΔHobs :
-76.86±n/a (kJ/mole)
Corrected for ΔHioniz:
not known
ΔCp :
-1.084±0.034 (kJ/mole)
ΔS° :
-0.127±0.01 (kJ/mole-K)
Comments:
data from Wibbenmeyer, J. A., Xavier, K. A., Smith-Gill, S. J., and Willson, R. C. (1999) Biochim. Biophys. Acta 1430, 191-202
Citation
 Wibbenmeyer, JA; Schuck, P; Smith-Gill, SJ; Willson, RC Salt links dominate affinity of antibody HyHEL-5 for lysozyme through enthalpic contributions. J Biol Chem 274:26838-42 (1999) [PubMed]  Article
Cell React
Source:
Recombinant HyHEL-5 Fab was expressed in E. coli.
Purity:
95%
Prep. Method:
Refolded protein was purified by Q Sepharose and nickel-charged chelating Sepharose Fast Flow Chromatography
Name:
HyHEL-5 Fab
Synonyms:
n/a
Type:
Antibody
Mol. Mass.:
23011.84
Organism:
Mus musculus (mouse)
Description:
Monoclonal antibody to hen egg lysozyme
Residue:
215
Sequence:
EVQLQQSGAELMKPGASVKISCKASGYTFSDYWIEWVKQRPGHGLEWIGEILPGSGSTNYHERFKGKATFTADTSSSTAYMQLNSLTSEDSGVYYCLHGNYDFDGWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKILD
  
Syringe React
Source:
Prepared from eggs purchased from Stevenson's Game Bird Farm (Riverside, TX)
Name:
Lysozyme (bobwhite quail)
Synonyms:
LYSC_COLVI | LYZ | bobwhite quail lysozyme(BQL)
Type:
Enzyme
Mol. Mass.:
14279.53
Organism:
Colinus virginianus
Description:
hydrolase
Residue:
129
Sequence:
KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNSQATNRNTDGSTDYGVLQINSRWWCNDGKTPGSRNLCNIPCSALLSSDITATVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL