Cell Reactant:
Acetate Operon Repressor (C-IclR)
Syringe Reactant:
BDBM19473
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
12/10/07
ΔG°:
30.1378±n/a (kJ/mole)
pH:
7.4±n/a
Log10Kb:
2.5
Temperature:
298.15±n/a (K)
ΔHobs :
-5.7266±n/a (kJ/mole)
Corrected for ΔHioniz:
no
Stoich. Param.:
0.5
ΔS° :
-0.1203±n/a (kJ/mole-K)
Citation
 Lorca, GLEzersky, ALunin, VVWalker, JRAltamentova, SEvdokimova, EVedadi, MBochkarev, ASavchenko, A Glyoxylate and pyruvate are antagonistic effectors of the Escherichia coli IclR transcriptional regulator. J Biol Chem 282:16476-91 (2007) [PubMed]  Article
Cell React
Source:
His-tagged fusion protein was overexpressed and purified in E. coli.
Prep. Method:
The His6 tag was cleaved from the protein by tobacco etch virus protease. The cleaved protein was then resolved by flowing the mixture through a second Ni2+ column. The purified proteins were dialyzed and concentrated.
Name:
Acetate Operon Repressor (IclR)
Synonyms:
n/a
Type:
Transcriptional regulator
Mol. Mass.:
29746.52
Organism:
Escherichia coli
Description:
n/a
Residue:
274
Sequence:
MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLLAIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASGAGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHALGLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR
  
Syringe React
Name:
BDBM19473
Synonyms:
2-Oxopropanoate | 2-oxopropanoic acid | Pyruvate
Type:
Small organic molecule
Emp. Form.:
C3H4O3
Mol. Mass.:
88.0621
SMILES:
CC(=O)C(O)=O
Structure:
Search PDB for entries with ligand similarity: