Target

Ligand

Substrate

Meas. Tech.

Scintillation Proximity Assay (SPA)

pH

7.4±n/a

Temperature

295.15±n/a K

Citation

 Lange, G; Lesuisse, D; Deprez, P; Schoot, B; Loenze, P; Benard, D; Marquette, JP; Broto, P; Sarubbi, E; Mandine, E Requirements for specific binding of low affinity inhibitor fragments to the SH2 domain of (pp60)Src are identical to those for high affinity binding of full length inhibitors. J Med Chem 46:5184-95 (2003) [PubMed]  Article Copy BDB DOI

More Info.:

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Name:

Proto-oncogene tyrosine-protein kinase Src [145-252]

Synonyms:

Proto-oncogene tyrosine-protein kinase Src | Proto-oncogene tyrosine-protein kinase Src (aa 145-252) | SRC | SRC1 | SRC_HUMAN | c-Src pp60c-src | p60-Src

Type:

SH2 Domain

Mol. Mass.:

12362.36

Organism:

Homo sapiens (Human)

Description:

Src-SH2 proteins (amino acid residues 145-252) were chemically biotinylated using a 10-fold molar excess of D-biotin-N-hydroxy-succinimidester and purified by gel filtration yielding conjugates with an average ratio of two biotin moieties per protein molecule.

Residue:

108

Sequence:

SIQAEEWYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYYSKHADGLCHRLTTVCPTSK

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Blast E-value cutoff:

Name:

BDBM14672

Synonyms:

2-hydroxypropane-1,2,3-tricarboxylic acid | CHEMBL1261 | Citrate | Citric Acid | Fragment 2 | cid_311

Type:

Small organic molecule

Emp. Form.:

C6H8O7

Mol. Mass.:

192.1235

SMILES:

OC(=O)CC(O)(CC(O)=O)C(O)=O

Structure:

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Similarity to this molecule at least:

Name:

Peptide Substrate

Synonyms:

n/a

Type:

Peptide

Mol. Mass.:

1600.79

Organism:

n/a

Description:

Peptide EPQpYEEIPIYL (middle-T antigen) was monoiodinated on tyrosine.

Residue:

13

Sequence:

IEPQPYEEIPIYL