| Assay Method Information | |
| | In Vitro Soybean Lipoxygenase (SLO) Inhibition Assay |
| Description: | SLO inhibition was studied by spectrophotometric assay by measuring the change in the absorbance at 234 nm due to formation of hydroperoxylinoleic acid from linoleic acid [Brathe et al., Bioorg. Med. Chem., 10(5):1581-1586]. Following are the compositions of the blank, control and sample. Blank: 700 μl sodium borate buffer (0.2 M, pH 8.0) was added to a solution of 50 μl of dimethyl sulfoxide (DMSO) and 250 μl of linoleic acid (250 μM solution in borate buffer) to make the reaction volume 1.0 ml. Control: Sodium borate buffer (200 μl, 0.2 M) was added to a solution of DMSO (50 μl), enzyme (500 μl, 400 units/ml in borate buffer) and linoleic acid (250 μl, 250 μM) to make the reaction volume 1.0 ml. Sample: Sodium borate buffer (200 μl, pH 8.0) was added to 50 μl of different concentrations of furan derivatives 3a-j (3.9-125 μg/ml in DMSO), enzyme (500 μl, 400 units/ml in borate buffer) and linoleic acid (250 μl) to make the reaction volume 1.0 ml. NDGA was used as a standard SLO inhibitor [Malterud et al., J. Agric. Food Chem., 48(11):5576-5580]. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |