Query String: ERK3 OE
BDBM573947 US11453697, Example 132 2-amino-9-[(5S,7R,8R,12aR,14R,15S,15aR)- 14-(6-amino-9H-purin-9-yl)-15-fluoro-2,10- dihydroxy-2,10-disulfidooctahydro-12H-5,8- methanofuro[3,2-l][1,3,6,9,11,2,10] pentaoxadiphosphacyclotetradecin-7-yl]-1,9- dihydro-6H-purin-6-oe (Diastereomer 3)
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- ChEMBL_616238 (CHEMBL1101131) Inhibition of ERK3
- ChEMBL_2495749 Inhibition of ERK3 (unknown origin)
- ChEMBL_655786 (CHEMBL1244830) Binding affinity to ERK3
- ChEMBL_586455 (CHEMBL1063710) Binding constant for ERK3 kinase domain
- ChEMBL_774367 (CHEMBL1908584) Binding constant for ERK3 kinase domain
- ChEMBL_982249 (CHEMBL2429316) Binding affinity to ERK3 (unknown origin)
- ChEMBL_2537957 Inhibition of human ERK3 by discoverX kinome scan assay
- ChEMBL_2047489 (CHEMBL4702188) Inhibition of ERK3 (unknown origin) expressed in HEK293 cells cotransfected with nano-luciferase by NanoBRET assay
- ChEMBL_2047480 (CHEMBL4702179) Binding affinity to wild-type human partial length ERK3 (M1 to P413 residues) expressed in bacterial expression system by kinomescan method
- ChEMBL_2047482 (CHEMBL4702181) Binding affinity to recombinant wild-type ERK3 (9 to 327 residues) (unknown origin) expressed in Escherichia coli by microscale thermophoresis assay
- ChEMBL_2047485 (CHEMBL4702184) Binding affinity to recombinant wild-type ERK3 (9 to 327 residues) (unknown origin) expressed in Sf21 insect cells by microscale thermophoresis assay
- ChEMBL_2047483 (CHEMBL4702182) Binding affinity to LPP-dephosphorylated recombinant wild-type ERK3 (9 to 327 residues) (unknown origin) expressed in Escherichia coli by microscale thermophoresis assay
- H6PD Activity Assay HEK293 H6PD-OE and HEK293 pellets were lysed and the lysate was quantified with the Bradford protein assay. Reaction mix containing galactose-6-phosphate (Gal6P) was combined with increasing concentrations of PARPi or DMSO. As an additional control, reaction mix without substrate was combined with HEK293 H6PD-OE lysate. Lysate from HEK293 or HEK293 H6PD-OE was added to each well to start the reactions. The absorbance at 340 nM (NADPH) was measured for 90 min at 10 min intervals. The 60 min data, which were within the linear range, was used for analysis. Background absorbance was subtracted and the H6PD activity of theHEK293 H6PD-OE lysates treated with PARPi was normalized to the DMSO control.
- ChEMBL_1874054 (CHEMBL4375343) Binding affinity to wild-type human partial length ERK3 (M1 to P413 residues) expressed in bacterial expression system measured after 1 hr by kinomescan method
- ChEMBL_2047488 (CHEMBL4702187) Inhibition of recombinant full length His6-GST fusion tagged ERK3 (1 to 721 residues) (unknown origin) using MK5 K51A mutant as substrate by ADP-Glo assay
- ChEMBL_2047486 (CHEMBL4702185) Inhibition of recombinant His6-GST fusion tagged ERK3 (1 to 365 residues) (unknown origin) expressed in Sf21 insect cells using MK5 K51A mutant as substrate by ADP-Glo assay
- ChEMBL_2047487 (CHEMBL4702186) Inhibition of recombinant His6-GST fusion tagged ERK3 (1 to 471 residues) (unknown origin) expressed in Sf21 insect cells using MK5 K51A mutant as substrate by ADP-Glo assay