CHEMBL1159714 Ko 707 BDBM50421719
3-((3S,6S)-6-Isobutyl-9-methoxy-1,4-dioxo-1,2,3,4,6,7,12,12a-octahydro-pyrazino[1',2':1,6]pyrido[3,4-b]indol-3-yl)-propionic acid tert-butyl ester BDBM50305083 3-((3S,6S,12aS)-6-Isobutyl-9-methoxy-1,4-dioxo-1,2,3,4,6,7,12,12a-octahydro-pyrazino[1',2':1,6]pyrido[3,4-b]indol-3-yl)-propionic acid tert-butyl ester Ko143 Ko-143 US9695174, Ko143 CHEMBL488910
- Bhattacharya, SK; Behenna, DC; Cameron, KO; Chen, P; Curto, JM; Freeman-Cook, KD; Jalaie, M; Kania, RS; Lian, Y; Nair, SK; Palmer, CL; Pettersson, MY; Rui, EY; Sammons, M; Yang, Q; Zhang, L US Patent US11203594 (2021)
- Pokhrel, L; Maezawa, I; Nguyen, TD; Chang, KO; Jin, LW; Hua, DH J Med Chem 55: 8969-73 (2012)
- Clausen, JD; Kjellerup, L; Cohrt, KO; Hansen, JB; Dalby-Brown, W; Winther, AL Bioorg Med Chem Lett 27: 4564-4570 (2017)
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- Zankel, TC; Isbell, SL; Ko, AA US Patent US10308607 (2019)
- Ko B
- Li, Z; Liao, C; Ko, BC; Shan, S; Tong, EH; Yin, Z; Pan, D; Wong, VK; Shi, L; Ning, ZQ; Hu, W; Zhou, J; Chung, SS; Lu, XP Bioorg Med Chem Lett 14: 3507-11 (2004)
- Ko, CC; Chen, YJ; Chen, CT; Liu, YC; Cheng, FC; Hsu, KC; Chow, LP J Biol Chem 289: 22078-89 (2014)
- Shih, KC; Shiau, CW; Chen, TS; Ko, CH; Lin, CL; Lin, CY; Hwang, CS; Tang, CY; Chen, WR; Huang, JW Bioorg Med Chem Lett 21: 4490-7 (2011)
- Ratni, H; Ebeling, M; Baird, J; Bendels, S; Bylund, J; Chen, KS; Denk, N; Feng, Z; Green, L; Guerard, M; Jablonski, P; Jacobsen, B; Khwaja, O; Kletzl, H; Ko, CP; Kustermann, S; Marquet, A; Metzger, F; Mueller, B; Naryshkin, NA; Paushkin, SV; Pinard, E; Poirier, A; Reutlinger, M; Weetall, M; Zeller, A; Zhao, X; Mueller, L J Med Chem 61: 6501-6517 (2018)
- Nam, Y; Ryu, KD; Jang, C; Moon, YH; Kim, M; Ko, D; Chung, KS; Gandini, MA; Lee, KT; Zamponi, GW; Lee, JY Bioorg Med Chem 28: (2020)
- Park, HK; Jeong, H; Ko, E; Lee, G; Lee, JE; Lee, SK; Lee, AJ; Im, JY; Hu, S; Kim, SH; Lee, JH; Lee, C; Kang, S; Kang, BH J Med Chem 60: 7569-7578 (2017)
- Brand, S; Ko, EJ; Viayna, E; Thompson, S; Spinks, D; Thomas, M; Sandberg, L; Francisco, AF; Jayawardhana, S; Smith, VC; Jansen, C; De Rycker, M; Thomas, J; MacLean, L; Osuna-Cabello, M; Riley, J; Scullion, P; Stojanovski, L; Simeons, FRC; Epemolu, O; Shishikura, Y; Crouch, SD; Bakshi, TS; Nixon, CJ; Reid, IH; Hill, AP; Underwood, TZ; Hindley, SJ; Robinson, SA; Kelly, JM; Fiandor, JM; Wyatt, PG; Marco, M; Miles, TJ; Read, KD; Gilbert, IH J Med Chem 60: 7284-7299 (2017)
- Cheng, MC; Li, CY; Ko, HC; Ko, FN; Lin, YL; Wu, TS J Nat Prod 69: 1305-9 (2006)
- Hillmann, P; Ko, GY; Spinrath, A; Raulf, A; von Kügelgen, I; Wolff, SC; Nicholas, RA; Kostenis, E; Höltje, HD; Müller, CE J Med Chem 52: 2762-75 (2009)
- An, S; Ko, H; Jang, H; Park, IG; Ahn, S; Hwang, SY; Gong, J; Oh, S; Kwak, SY; Lee, Y; Kim, H; Noh, M ACS Med Chem Lett 14: 425-431 (2023)
- Cheng, MC; Li, CY; Ko, HC; Ko, FN; Lin, YL; Wu, TS J Nat Prod 69: 1305-9 (2006)
- Ng, LT; Ko, HH; Lu, TM Bioorg Med Chem 17: 4360-6 (2009)
- Choi, H; Park, HJ; Shin, JC; Ko, HS; Lee, JK; Lee, S; Park, H; Hong, S Bioorg Med Chem Lett 22: 2195-9 (2012)
- Ko HY
- Ko J
- Ko, JH; Yeon, SW; Ryu, JS; Kim, TY; Song, EH; You, HJ; Park, RE; Ryu, CK Bioorg Med Chem Lett 16: 6001-5 (2006)
- Ko, K; Kim, HJ; Ho, PS; Lee, SO; Lee, JE; Min, CR; Kim, YC; Yoon, JH; Park, EJ; Kwon, YJ; Yun, JH; Yoon, DO; Kim, JS; Park, WS; Oh, SS; Song, YM; Cho, WK; Morikawa, K; Lee, KJ; Park, CH J Med Chem 61: 2949-2961 (2018)
- Lee, W; Ko, KR; Kim, HK; Lee, DS; Nam, IJ; Lim, S; Kim, S J Nat Prod 81: 1343-1356 (2018)
- Ko, KS; Steffey, ME; Brandvold, KR; Soellner, MB ACS Med Chem Lett 4: 779-783 (2013)
- Jeon, S; Ko, M; Lee, J; Choi, I; Byun, SY; Park, S; Shum, D; Kim, S Antimicrob Agents Chemother 64: (2020)
- Kim, Y; Kim, J; Kim, S; Ki, Y; Seo, SH; Tae, J; Ko, MK; Jang, HS; Lim, EJ; Song, C; Cho, Y; Koh, HY; Chong, Y; Choo, IH; Keum, G; Min, SJ; Choo, H Eur J Med Chem 85: 629-37 (2014)
- Jackson, JJ; Shibuya, GM; Ravishankar, B; Adusumilli, L; Bradford, D; Brockstedt, DG; Bucher, C; Bui, M; Cho, C; Colas, C; Cutler, G; Dukes, A; Han, X; Hu, DX; Jacobson, S; Kassner, PD; Katibah, GE; Ko, MYM; Kolhatkar, U; Leger, PR; Ma, A; Marshall, L; Maung, J; Ng, AA; Okano, A; Pookot, D; Poon, D; Ramana, C; Reilly, MK; Robles, O; Schwarz, JB; Shakhmin, AA; Shunatona, HP; Sreenivasan, R; Tivitmahaisoon, P; Xu, M; Zaw, T; Wustrow, DJ; Zibinsky, M J Med Chem 65: 12895-12924 (2022)
- Ko, S; Lee, MK; Shin, D; Park, H Bioorg Med Chem 17: 7769-74 (2009)
- Lim, CJ; Woo, SE; Ko, SI; Lee, BH; Oh, KS; Yi, KY Bioorg Med Chem Lett 26: 4684-4686 (2016)
- Hwang, GJ; Jang, M; Son, S; Lee, B; Jang, JP; Lee, JS; Ko, SK; Hong, YS; Ahn, JS; Jang, JH J Nat Prod 84: 2420-2426 (2021)
- Patel, M; McHugh, RJ; Cordova, BC; Klabe, RM; Erickson-Viitanen, S; Trainor, GL; Ko, SS Bioorg Med Chem Lett 9: 3221-4 (1999)
- Dziadulewicz, EK; Ritchie, TJ; Hallett, A; Snell, CR; Ko, SY; Wrigglesworth, R; Hughes, GA; Dunstan, AR; Bloomfield, GC; Drake, GS; Brown, MC; Lee, W; Burgess, GM; Davis, C; Yaqoob, M; Perkins, MN; Campbell, EA; Davis, AJ; Rang, HP J Med Chem 43: 769-71 (2000)
- Guo, RT; Cao, R; Liang, PH; Ko, TP; Chang, TH; Hudock, MP; Jeng, WY; Chen, CK; Zhang, Y; Song, Y; Kuo, CJ; Yin, F; Oldfield, E; Wang, AH Proc Natl Acad Sci U S A 104: 10022-7 (2007)
- Quang, TH; Ngan, NT; Ko, W; Kim, DC; Yoon, CS; Sohn, JH; Yim, JH; Kim, YC; Oh, H Bioorg Med Chem Lett 24: 5787-91 (2014)
- Ryu, CK; Kang, HY; Lee, SK; Nam, KA; Hong, CY; Ko, WG; Lee, BH Bioorg Med Chem Lett 10: 461-4 (2000)
- Ko Y
- Jeon, WS; Moon, K; Park, SH; Chun, H; Ko, YH; Lee, JY; Lee, ES; Samal, S; Selvapalam, N; Rekharsky, MV; Sindelar, V; Sobransingh, D; Inoue, Y; Kaifer, AE; Kim, K J Am Chem Soc 127: 12984-9 (2005)
- Kim, YK; Kwon, O; Park, H; Park, J; Choi, HG; Son, JB; Ko, E; Kim, SY; Lee, S; Kang, SY; Ko, YK; Park, J US Patent US11447480 (2022)
- Humblet, V; Misra, P; Bhushan, KR; Nasr, K; Ko, YS; Tsukamoto, T; Pannier, N; Frangioni, JV; Maison, W J Med Chem 52: 544-50 (2009)
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- Raddatz, P; Jonczyk, A; Minck, KO; Schmitges, CJ; Sombroek, J J Med Chem 34: 3267-80 (1991)
- Shaaban, MA; Elshaier, YAMM; Hammad, AH; Farag, NA; Hassan Haredy, H; AbdEl-Ghany, AA; Mohamed, KO Bioorg Med Chem Lett 30: (2020)
- Mohammed, KO; Nissan, YM Chem Biol Drug Des 84: 473-88 (2014)
- Bugge, S; Moen, IU; Sylte, KO; Sundby, E; Hoff, BH Eur J Med Chem 94: 175-94 (2015)
- Narayanan, D; Tran, KT; Pallesen, JS; Solbak, SMØ; Qin, Y; Mukminova, E; Luchini, M; Vasilyeva, KO; González Chichón, D; Goutsiou, G; Poulsen, C; Haapanen, N; Popowicz, GM; Sattler, M; Olagnier, D; Gajhede, M; Bach, A J Med Chem 65: 14481-14526 (2022)
- Yerdelen, KO; Koca, M; Anil, B; Sevindik, H; Kasap, Z; Halici, Z; Turkaydin, K; Gunesacar, G Bioorg Med Chem Lett 25: 5576-82 (2015)
- Pontius, A; Krick, A; Mesry, R; Kehraus, S; Foegen, SE; Mu¨ller, M; Klimo, K; Gerha¨user, C; Ko¨nig, GM J Nat Prod 71: 1793-1799 (2008)
- Mattei, P; Boehringer, M; Di Giorgio, P; Fischer, H; Hennig, M; Huwyler, J; Koçer, B; Kuhn, B; Loeffler, BM; Macdonald, A; Narquizian, R; Rauber, E; Sebokova, E; Sprecher, U Bioorg Med Chem Lett 20: 1109-13 (2010)
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- ZakoSek, M; Mihevc, SP; Majdic, G; Ko{hacek over (s)}ak, U; Gobec, S US Patent US20230331674 (2023)
- ChEMBL_598674 (CHEMBL1050755) Agonist activity at GIPR
- ChEMBL_795449 (CHEMBL1937454) Displacement of [125I]GIP from human GIPR
- ChEMBL_513225 (CHEMBL977355) Binding affinity to GIPR in rat liver membrane
- ChEMBL_513212 (CHEMBL977342) Binding affinity to human cloned GIPR expressed in BHK cells
- ChEMBL_1338756 (CHEMBL3242379) Antagonist activity at human GIPR assessed as inhibition of cAMP production
- ChEMBL_2223097 (CHEMBL5136431) Positive allosteric modulator activity at human GIPR expressed in HEK293 cells assessed as potentiation of intracellular cAMP accumulation incubated for 30 mins in presence of GIPR agonist GIP(I-42) by HTRF assay
- ChEMBL_2100497 (CHEMBL4808893) Antagonist activity at human GIPR expressed in HEK293 cells assessed as inhibition of GIP-induced cAMP production
- ChEMBL_701138 (CHEMBL1648236) Antagonist activity at human GIPR expressed in CHO cells assessed as inhibition of glucagon-induced cAMP accumulation
- ChEMBL_2100503 (CHEMBL4808899) Antagonist activity at mouse GIPR expressed in CHO-K1 cells assessed as inhibition of GIP-induced cAMP production
- ChEMBL_2476844 Inhibition of tetracycline-inducible FLAG-tagged human PARL stably transfected in HEK293T harboring FITR/PARL KO
- ChEMBL_480051 (CHEMBL927990) Inhibition of mouse PKCtheta in KO cells assessed as blockade of anti CD28-stimulated IL2 production
- ChEMBL_2154863 (CHEMBL5039523) Agonist activity at STING KO human THP-1 dual cells incubated for 20 hrs by luciferase reporter gene assay
- ChEMBL_2322358 Agonist activity at STING in human STHP1-Dual KO-STING cells incubated for 20 hrs by Quanti-luc reagent based assay
- ChEMBL_2354137 Agonist activity at STING in PMA-differentiated human THP1-Dual KO-STING cells incubated for 24 hrs by QUANTI-Blue assay
- ChEMBL_2473762 Inhibition of PRMT5 in human HCT-116 cells with MTAP KO assessed as decrease in SMDA level incubated for 48 hrs by immunofluorescence analysis
- ChEMBL_857258 (CHEMBL2160603) Antagonist activity at human GIPR expressed in CHO cells assessed as inhibition of glucagon-induced cAMP accumulation preincubated for 30 mins prior to glucagon challenge measured after 30 mins post glucagon challenge by liquid scintillation counter
- ChEMBL_2160781 (CHEMBL5045531) Agonist activity at STING in human THP1 Dual KO-STING cells assessed as IRF reporter activation incubated for 20 hrs by quanti-blue SEAP reporter gene assay
- ChEMBL_2160782 (CHEMBL5045532) Agonist activity at STING in mouse RAW-Lucia ISG-KO-STING cells assessed as IRF reporter activation incubated for 20 hrs by quanti-blue SEAP reporter gene assay
- ChEMBL_2160784 (CHEMBL5045534) Agonist activity at STING in human THP1-Dual KO-STING cells assessed as NF-kappaB reporter activation incubated for 20 hrs by quanti-blue SEAP reporter gene assay
- Determination of the value of binding affinity constant (KO between the compound and BRD4 BD2 protein The purity of BRD4 BD2 protein used in the experiment was greater than 95%, and the protein concentration was 46.33 uM. The 96-well plate was purchased from Corning (black, #3694). The multifunctional microplate reader was a product of TECAN, model: SPARK 10M. Buffer: 100 mM potassium phosphate (pH 6.5), 2% ethylene glycol (Sigma) and 0.01% Trition X-100 (Sigma). The experimental water was Millipore-Q pure water.The Ki value of the compound and BRD4 BD2 protein was measured according to the FP test procedures for detecting the Ki value of the compound and BRD4 BD1 protein except that the BRD4 BD1 protein was replaced with the BRD4 BD2 protein.
- Determination of the value of binding affinity constant (KO between the compound and BRD4 BD1 protein The purity of BRD4 BD1 protein used in the experiment was greater than 95%, and the protein concentration was 43.4 uM. The 96-well plate was purchased from Corning (black, #3694). The multifunctional microplate reader was a product of TECAN, model: SPARK 10M. Buffer: 100 mM potassium phosphate (pH 6.5), 2% ethylene glycol (Sigma) and 0.01% Trition X-100 (Sigma). The experimental water was Millipore-Q pure water.The specific experimental steps were as follows.First, the compound to be tested was dissolved in ethylene glycol to prepare into a 10 mM standard stock solution. Subsequently, the standard stock solution of the compound to be tested was diluted into a working sample solution with the buffer in an EP tube and ready for use. The concentration of the prepared working sample solution was 5 times of the highest sample concentration required on the test plate (5×test compound solution).40 λL of a 5× test compound solution of a sample A was added to wells B1-B3 of a 96-well plate, and 40 μL of a 5× test compound solution of a sample B was added to wells B7-B9 of the 96-well plate, respectively. 20 uL of the buffer was added to the remaining wells, except for wells B1-B3 and B7-B9. Then, 20 uL of a solution was taken from wells B1-B3 to C1-C3, and this 2-fold dilution was repeated from C1-C3 until H4-H6; in the same way, 20 uL of a solution was taken from B7-B9 to C7-C9, this 2-fold dilution was repeated from C7-C9 until H10-H12. Finally, 80 uL of a mixed solution containing 2.5 nM Tracer and 37.5 nM BRD4 BD1 protein was added to each well.