colforsina (3R,4aR,5S,6S,6aS,10S,10aR,10bS)-3-ethenyl-6,10,10b-trihydroxy-3,4a,7,7,10a-pentamethyl-1-oxododecahydro-1H-benzo[f]chromen-5-yl acetate CHEMBL52606 colforsine colforsinum 7beta-acetoxy-8,13-epoxy-1alpha,6beta,9alpha-trihydroxylabd-14-en-11-one FORSKOLIN cid_47936 BDBM50010261
- Robbins, JD; Laurenza, A; Kosley, RW; O'Malley, GJ; Spahl, B; Seamon, KB (Aminoalkyl)carbamates of forskolin: intermediates for the synthesis of functionalized derivatives of forskolin with different specificities for adenylyl cyclase and the glucose transporter. J Med Chem 34: 3204-12 (1991)
- Robbins, JD; Boring, DL; Tang, WJ; Shank, R; Seamon, KB Forskolin carbamates: binding and activation studies with type I adenylyl cyclase. J Med Chem 39: 2745-52 (1996)
- Chijiwa, T; Mishima, A; Hagiwara, M; Sano, M; Hayashi, K; Inoue, T; Naito, K; Toshioka, T; Hidaka, H Inhibition of forskolin-induced neurite outgrowth and protein phosphorylation by a newly synthesized selective inhibitor of cyclic AMP-dependent protein kinase, N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H-89), of PC12D pheochromocytoma cells. J Biol Chem 265: 5267-72 (1990)
- ChEMBL_143823 (CHEMBL748547) Ability to reverse Neuropeptide Y receptor type 1-induced inhibition of forskolin-induced inhibition of forskolin-stimulated cAMP
- ChEMBL_1459251 (CHEMBL3367481) Inhibition of forskolin-stimulated CFTR (unknown origin)
- ChEMBL_634017 (CHEMBL1117990) Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge
- ChEBML_218835 Inhibition of forskolin-stimulated cAMP accumulation in CHO cells expressing hmGlu4a
- ChEMBL_517 (CHEMBL615539) Inhibition of forskolin-stimulated lipolysis in differentiated 3T3-L1 cells
- ChEMBL_1973 (CHEMBL617578) Inhibition of forskolin stimulated adenylate cyclase at 5-hydroxytryptamine 1D receptor
- ChEMBL_218833 (CHEMBL823944) Inhibition of forskolin-stimulated cAMP accumulation in CHO cells expressing hmGlu4a
- ChEMBL_218835 (CHEMBL823946) Inhibition of forskolin-stimulated cAMP accumulation in CHO cells expressing hmGlu4a
- ChEMBL_2214410 (CHEMBL5127542) Agonist activity at human MOP assessed as forskolin stimulated cAMP accumulation
- ChEMBL_509903 (CHEMBL1002173) Agonist activity against human GPR109a assessed as forskolin-induced cAMP accumulation
- ChEMBL_509905 (CHEMBL1002175) Agonist activity against mouse GPR109a assessed as forskolin-induced cAMP accumulation
- ChEMBL_536823 (CHEMBL988087) Activity at GPR109a assessed as inhibition of forskolin-stimulated cAMP production
- ChEMBL_536824 (CHEMBL988088) Activity at GPR109b assessed as inhibition of forskolin-stimulated cAMP production
- ChEMBL_810 (CHEMBL767044) Inhibition of forskolin stimulated adenylate cyclase at 5-hydroxytryptamine 1A receptor
- ChEMBL_1334788 (CHEMBL3238895) Agonist activity at human CB2 receptor assessed as inhibition of forskolin-induced cAMP production preincubated for 15 mins followed by forskolin challenge measured after 30 mins by HTRF assay
- ChEMBL_1334790 (CHEMBL3238897) Agonist activity at human CB1 receptor assessed as inhibition of forskolin-induced cAMP production preincubated for 15 mins followed by forskolin challenge measured after 30 mins by HTRF assay
- ChEMBL_1825154 (CHEMBL4324918) Agonist activity at mouse mu opioid receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP formation incubated for 8 min followed by forskolin stimulation by ELISA
- ChEMBL_106231 (CHEMBL715057) Effective concentration forskolin-stimulated cAMP formation in BHK cells expressing mGluR2 receptor
- ChEMBL_31878 (CHEMBL645668) Inhibition of [125 I]6-IHPP-forskolin binding to adenylate cyclase 1
- ChEMBL_31879 (CHEMBL645669) Inhibition of [125 I]6-IHPP-forskolin binding to adenylate cyclase 1
- ChEMBL_31880 (CHEMBL645670) Inhibition of [125 I]6-IHPP-forskolin binding to Adenylate cyclase 1
- ChEMBL_87223 (CHEMBL696444) Inhibition of the forskolin-stimulated cAMP production for human Histamine H4 receptor
- ChEBML_1689990 Agonist activity at recombinant human KOR expressed in HEK293T cells assessed as inhibition of forskolin-stimulated cAMP level preincubated for 15 to 20 mins followed by forskolin addition by GloSensor assay
- ChEMBL_1907313 (CHEMBL4409671) Antagonist activity at 5HT7R receptor (unknown origin) assessed as forskolin-mediated cAMP accumulation preincubated for 30 mins followed by forskolin addition and measured after 30 mins by TR-FRET analysis
- ChEMBL_1689990 (CHEMBL4040560) Agonist activity at recombinant human KOR expressed in HEK293T cells assessed as inhibition of forskolin-stimulated cAMP level preincubated for 15 to 20 mins followed by forskolin addition by GloSensor assay
- ChEMBL_1779729 (CHEMBL4236721) Agonist activity at human dopamine D2 receptor expressed in HEK293T cells assessed as reduction in forskolin-induced cAMP accumulation preincubated for 15 mins followed by forskolin addition by GloSensor-based assay
- ChEMBL_743422 (CHEMBL1767776) Agonist activity at human CB2 receptor expressed in CHOK1 cells assessed as inhibition of forskolin-induced cAMP production treated 15 mins before forskolin challenge measured after 1 hr by HTRF assay
- ChEMBL_743423 (CHEMBL1767777) Agonist activity at human CB1 receptor expressed in CHOK1 cells assessed as inhibition of forskolin-induced cAMP production treated 15 mins before forskolin challenge measured after 1 hr by HTRF assay
- ChEMBL_743428 (CHEMBL1767782) Agonist activity at rat CB2 receptor expressed in CHOK1 cells assessed as inhibition of forskolin-induced cAMP production treated 15 mins before forskolin challenge measured after 1 hr by HTRF assay
- ChEMBL_743486 (CHEMBL1767886) Agonist activity at rat CB1 receptor expressed in CHOK1 cells assessed as inhibition of forskolin-induced cAMP production treated 15 mins before forskolin challenge measured after 1 hr by HTRF assay
- ChEMBL_793417 (CHEMBL1932021) Agonist activity at human 5HT1A receptor expressed in human HeLa cells assessed as inhibition of forskolin-stimulated cAMP production pretreated 20 mins before forskolin challenge measured after 2 hrs by RIA
- ChEMBL_808311 (CHEMBL1961328) Inhibition of PDE4B expressed in HEK293 cells assessed as fold reduction in forskolin-stimulated cAMP production pretreated for 30 mins measured 4 hrs after forskolin challenge by luciferase reporter gene assay
- ChEBML_104938 Agonist activity for its ability to inhibit forskolin-stimulated cAMP accumulation against MT receptor
- ChEMBL_218859 (CHEMBL822316) Inhibition of forskolin stimulated cAMP production in RGT cells expressing recombinant human mGluR1a
- ChEMBL_219016 (CHEMBL818784) Effective concentration against forskolin-stimulated cAMP formation in BHK cells expressing mGluR4 receptor
- ChEMBL_219019 (CHEMBL818787) Inhibition of forskolin stimulated cAMP production in RGT cells expressing recombinant human mGluR4a
- ChEMBL_219028 (CHEMBL818796) Inhibition of forskolin stimulated cAMP production in RGT cells expressing recombinant human mGluR5a
- ChEMBL_219029 (CHEMBL818797) Inhibition of forskolin stimulated cAMP production in RGT cells expressing recombinant human mGluR5a
- ChEMBL_219034 (CHEMBL819438) Inhibition of forskolin stimulated cAMP production in RGT cells expressing recombinant human mGluR6
- ChEMBL_219038 (CHEMBL819587) Inhibition of forskolin stimulated cAMP production in RGT cells expressing recombinant human mGluR7
- ChEMBL_219041 (CHEMBL824614) Inhibition of forskolin stimulated cAMP production in RGT cells expressing recombinant human mGluR8
- ChEMBL_33812 (CHEMBL647196) Alpha2c receptor agonism measured as ability to inhibit forskolin-stimulated synthesis of cAMP
- ChEMBL_456679 (CHEMBL924060) Agonist activity at human cloned GPR109a receptor by forskolin-stimulated cAMP production test
- ChEBML_1697694 Agonist activity at human A1AR expressed in FlpIn-CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation preincubated for 10 mins followed by forskolin addition measured after 30 mins by Alphascreen assay
- ChEBML_1697703 Agonist activity at human A2BAR expressed in FlpIn-CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation preincubated for 40 mins followed by forskolin addition measured after 30 mins by Alphascreen assay
- ChEBML_1697706 Agonist activity at human A3AR expressed in FlpIn-CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation preincubated for 10 mins followed by forskolin addition measured after 30 mins by Alphascreen assay
- ChEMBL_1436814 (CHEMBL3386599) Agonist activity at APJ receptor in HEK293 cells assessed as inhibition of forskolin-induced cAMP level incubated for 5 mins prior to forskolin challenge measured after 30 mins by TR-FRET assay
- ChEMBL_1700353 (CHEMBL4051335) Agonist activity at human kappa-type opioid receptor expressed in CHO-K1 cells assessed as forskolin-induced cAMP accumulation after 30 mins in presence of forskolin by luminescence-based HitHunter cAMP assay
- ChEMBL_1731764 (CHEMBL4147300) Neutral antagonist activity at human CB2 assessed as restoration of forskolin-induced cAMP formation preincubated for 15 mins followed by forskolin addition for 30 mins measured after 1 hr by HTRF assay
- ChEMBL_1798205 (CHEMBL4270322) Antagonist activity at human SSR5 expressed in CHOK1 cells assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 15 mins followed by forskolin addition measured after 1 hr by LANCE assay
- ChEMBL_1798207 (CHEMBL4270324) Antagonist activity at mouse SSR5 expressed in CHOK1 cells assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 15 mins followed by forskolin addition measured after 1 hr by LANCE assay
- ChEBML_1697700 Agonist activity at human A2A-AR expressed in FlpIn-CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation preincubated for 40 mins followed by forskolin addition measured after 30 mins by Alphascreen assay
- ChEMBL_1689972 (CHEMBL4040542) Agonist activity at human CB2 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 20 mins followed by forskolin addition measured after 30 mins by HTRF assay
- ChEMBL_1697694 (CHEMBL4048584) Agonist activity at human A1AR expressed in FlpIn-CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation preincubated for 10 mins followed by forskolin addition measured after 30 mins by Alphascreen assay
- ChEMBL_1906924 (CHEMBL4409282) Inhibition of mu opioid receptor (unknown origin) expressed in HEK293 cells assessed as inhibition of forskolin-stimulated intracellular cAMP accumulation preincubated for 30 mins followed by forskolin-stimulation and measured after 60 mins
- ChEMBL_2057167 (CHEMBL4712168) Agonist activity at human GPR84 expressed in CHO cells assessed as reduction in forskolin-stimulated cAMP production preincubated for 20 mins followed by forskolin stimulation and measured after 20 mins by cAMP assay
- ChEMBL_2169530 (CHEMBL5054589) Positive allosteric modulation of human CB1 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP production incubated for 30 mins in presence of forskolin by HitHunter chemiluminescence based assay
- ChEMBL_812529 (CHEMBL2014404) Agonist activity at human recombinant adenosine receptor-1 expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production preincubated for 45 mins prior forskolin-induction measured after 15 mins by immunoassay
- ChEMBL_879383 (CHEMBL2208781) Antagonist activity at human histamine H3 receptor expressed in human HEK293 cells assessed as inhibition of forskolin-induced cAMP release pretreated 10 mins prior forskolin stimulation measured after 4.5 hrs by spectrofluorimetric analysis
- ChEMBL_879384 (CHEMBL2208782) Antagonist activity at rat histamine H3 receptor expressed in human HEK293 cells assessed as inhibition of forskolin-induced cAMP release pretreated 10 mins prior forskolin stimulation measured after 4.5 hrs by spectrofluorimetric analysis
- ChEMBL_104771 (CHEMBL714916) Agonist activity for its ability to inhibit forskolin-stimulated cAMP accumulation against MT1 receptor
- ChEMBL_1334791 (CHEMBL3238898) Agonist activity at human CB2 receptor assessed as inhibition of forskolin-induced cAMP production
- ChEMBL_1334792 (CHEMBL3238899) Agonist activity at human CB1 receptor assessed as inhibition of forskolin-induced cAMP production
- ChEMBL_1426 (CHEMBL616506) Inhibition constant of 50 uM forskolin-stimulated cAMP accumulation against 5-hydroxytryptamine 1A receptor
- ChEMBL_1444655 (CHEMBL3376653) Agonist activity at rat CB2 receptor assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_1444656 (CHEMBL3376654) Agonist activity at rat CB1 receptor assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_145690 (CHEMBL753316) Inhibition of Forskolin-Stimulated Adenylyl Cyclase in CHO cells expressing Opioid receptor kappa 1
- ChEMBL_2214475 (CHEMBL5127607) Agonist activity at KOP (unknown origin) assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_310298 (CHEMBL832303) Inhibition of forskolin-induced cAMP accumulation in CHO-K1 cells expressing human sst5 receptor
- ChEMBL_310308 (CHEMBL833264) Inhibition of forskolin-induced cAMP accumulation in CHO-K1 cells expressing human sst2 receptor
- ChEMBL_429281 (CHEMBL915842) Activity at GPR109a in CHO cells assessed as inhibition of forskolin-induced cAMP generation
- ChEMBL_429282 (CHEMBL915841) Activity at GPR109b in CHO cells assessed as inhibition of forskolin-induced cAMP generation
- ChEMBL_552265 (CHEMBL995595) Agonist activity at human CB2 receptor assessed as inhibition of forskolin-stimulated cAMP production
- ChEMBL_1295162 (CHEMBL3128663) Partial agonist activity at human CRTH2 receptor expressed in HEK cells assessed as forskolin-induced cAMP formation preincubated for 10 mins followed by forskolin challenge measured after 10 to 60 mins by ELISA assay
- ChEMBL_1589778 (CHEMBL3830330) Agonist activity at GPR84 (unknown origin) expressed in in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation preincubated for 30 mins followed by forskolin addition measured after 30 mins by HTRF assay
- ChEMBL_1661888 (CHEMBL4011500) Agonist activity at human adenosine A3 receptor expressed in CHO cells assessed as decrease in forskolin induced cAMP formation preincubated for 10 mins followed by forskolin challenge measured after 15 mins by AlphaScreen assay
- ChEMBL_1665431 (CHEMBL4015227) Agonist activity at human N-terminal-FLAG-tagged human GPR84 expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation preincubated fro 20 mins followed by forskolin addition measured after 20 mins
- ChEMBL_1704594 (CHEMBL4055827) Antagonist activity at human SSTR5 expressed in CHO cells assessed as inhibition of SST14-induced forskolin-stimulated intracellular cAMP level incubated for 15 mins followed by forskolin stimulation for 30 mins by HTRF assay
- ChEMBL_1704595 (CHEMBL4055828) Antagonist activity at mouse SSTR5 expressed in CHO cells assessed as inhibition of SST14-induced forskolin-stimulated intracellular cAMP level incubated for 15 mins followed by forskolin stimulation for 30 mins by HTRF assay
- ChEMBL_1704986 (CHEMBL4056219) Antagonist activity at human SSTR5 expressed in CHO cells assessed as inhibition of SST14-induced forskolin-stimulated intracellular cAMP level incubated for 15 mins followed by forskolin stimulation for 30 mins by HTRF assay
- ChEMBL_1704987 (CHEMBL4056220) Antagonist activity at mouse SSTR5 expressed in CHO cells assessed as inhibition of SST14-induced forskolin-stimulated intracellular cAMP level incubated for 15 mins followed by forskolin stimulation for 30 mins by HTRF assay
- ChEMBL_1779730 (CHEMBL4236722) Agonist activity at human dopamine D2 receptor /mGluR5a (unknown origin) expressed in HEK293T cells assessed as reduction in forskolin-induced cAMP accumulation preincubated for 15 mins followed by forskolin addition by GloSensor-based assay
- ChEMBL_1867253 (CHEMBL4368228) Agonist activity at human D2S receptor expressed in CHOK1 cells assessed as inhibition of forskolin induced cAMP production incubated for 10 mins followed by forskolin addition and measured after 5 mins by HTRF assay
- ChEMBL_1892881 (CHEMBL4394802) Agonist activity at recombinant human Gs-coupled H2 receptor expressed in HEK293T cells assessed as inhibition of forskolin-stimulated cAMP accumulation preincubated for 10 to 15 followed by forskolin-stimulation by Glo-sensor assay
- ChEMBL_1899970 (CHEMBL4402085) Agonist activity at human RXFP3 expressed in CHOK1 cells assessed as inhibition of forskolin-stimulated intracellular cAMP accumulation preincubated for 30 mins followed by forskolin-stimulation and measured after 30 mins by HTRF assay
- ChEMBL_1899973 (CHEMBL4402088) Agonist activity at human RXFP4 expressed in CHOK1 cells assessed as inhibition of forskolin-stimulated intracellular cAMP accumulation preincubated for 30 mins followed by forskolin-stimulation and measured after 30 mins by HTRF assay
- ChEMBL_1906925 (CHEMBL4409283) Inhibition of mu opioid receptor (unknown origin) expressed in HEK293 cells assessed as inhibition of forskolin-stimulated beta arrestin 2 recruitment preincubated for 30 mins followed by forskolin-stimulation and measured after 60 mins
- ChEMBL_970290 (CHEMBL2406816) Agonist activity at mouse adenosine A1 receptor expressed in HEK293 cell membranes assessed as inhibition of forskolin-stimulated cAMP production preincubated for 30 mins prior to forskolin-treatment measured after 15 mins by ELISA
- ChEMBL_970296 (CHEMBL2403962) Agonist activity at mouse adenosine A3 receptor expressed in HEK293 cell membranes assessed as inhibition of forskolin-stimulated cAMP production preincubated for 30 mins prior to forskolin-treatment measured after 15 mins by ELISA
- ChEMBL_1295164 (CHEMBL3128665) Antagonist activity at human CRTH2 receptor expressed in HEK cells assessed as inhibition of forskolin-induced cAMP formation preincubated for 10 mins followed by forskolin challenge measured after 10 to 60 mins by ELISA assay
- ChEMBL_1579563 (CHEMBL3811073) Agonist activity at human nociceptin receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production preincubated for 15 mins followed by forskolin addition measured for 90 mins by luciferase reporter gene assay
- ChEMBL_1624991 (CHEMBL3867403) Agonist activity at human PAR2 expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 20 mins followed by forskolin addition for 10 mins measured after 1 hr by LANCE assay
- ChEMBL_1663195 (CHEMBL4012876) Agonist activity at human adenosine receptor A2B expressed in CHO cell membranes assessed as forskolin-stimulated cAMP accumulation preincubated for 20 mins followed by forskolin addition measured after 10 mins by liquid scintillation counter method
- ChEMBL_1855676 (CHEMBL4356405) Agonist activity at human H3R expressed on SK-N-MC cells assessed as inhibition of forskolin-induced beta galactosidase activity preincubated with forskolin for 6 hrs followed compound addition by CRE-luciferase reporter gene assay
- ChEMBL_1870479 (CHEMBL4371646) Agonist activity at human adenosine A3 receptor expressed in FlpIn-CHO cells assessed as inhibition of forskolin-mediated cAMP accumulation preincubated for 10 mins followed by forskolin addition measured after 30 mins by alphascreen assay
- ChEMBL_2090990 (CHEMBL4772253) Agonist activity at human nociceptin receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 15 mins followed by forskolin addition in presence of IBMX by Glo-sensor cAMP assay
- ChEMBL_2186473 (CHEMBL5098555) Agonist activity at recombinant human CB1 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 15 mins followed by forskolin stimulation and measured after 15 mins by HTRF assay
- ChEMBL_2186474 (CHEMBL5098556) Agonist activity at recombinant human CB2 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 15 mins followed by forskolin stimulation and measured after 15 mins by HTRF assay
- ChEMBL_590641 (CHEMBL1053707) Agonist activity at human recombinant adenosine A3 receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production treated 45 mins before forskolin challenge measured after 15 mins by radioimmunoassay relative to NECA
- ChEMBL_833206 (CHEMBL2067497) Agonist activity at human recombinant adenosine A3 receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production incubated for 30 mins prior to forskolin-stimulation measured after 15 mins by enzyme immunoassay
- ChEMBL_970293 (CHEMBL2406819) Partial agonist activity at mouse adenosine A1 receptor expressed in HEK293 cell membranes assessed as inhibition of forskolin-stimulated cAMP production preincubated for 30 mins prior to forskolin-treatment measured after 15 mins by ELISA
- ChEBML_1768 Inhibition of Forskolin-stimulated adenylate cyclase activity against 5-hydroxytryptamine 1B receptor of rat substantia nigra
- ChEBML_1972 Inhibition of Forskolin-stimulated adenylate cyclase activity against 5-hydroxytryptamine 1D receptor of rat substantia nigra
- ChEBML_219011 Compound was evaluated for the inhibition of forskolin stimulated cAMP accumulation in CHO cells expressing hmGluR4a
- ChEBML_219039 Compound was evaluated for the inhibition of forskolin stimulated cAMP accumulation in CHO cells expressing hmGluR7b
- ChEBML_344944 Agonist activity at human NPY4 receptor in HEK293 cells by inhibition of forskolin-stimulated cAMP synthesis
- ChEBML_344945 Agonist activity at human NPY1 receptor in CHO cells by inhibition of forskolin-stimulated cAMP synthesis
- ChEBML_344947 Agonist activity at human NPY2 receptor in CHO cells by inhibition of forskolin-stimulated cAMP synthesis
- ChEBML_593 Effect on forskolin stimulated adenylate cyclase activity at 5-hydroxytryptamine 1A receptor of guinea pig hippocampus.
- ChEBML_604 Inhibition of Forskolin-stimulated adenylate cyclase activity against 5-hydroxytryptamine 1A receptor of guinea pig hippocampus
- ChEMBL_105899 (CHEMBL717760) Inhibition of forskolin stimulated cAMP production in RGT cells expressingrecombinant human Metabotropic glutamate receptor 2
- ChEMBL_144880 (CHEMBL749953) Inhibition of forskolin stimulated cAMP accumulation in CHO cells stably expressing the human OP4 receptor
- ChEMBL_1870458 (CHEMBL4371625) Agonist activity at APJ receptor (unknown origin) assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_2075854 (CHEMBL4731388) Agonist activity at human delta opioid receptor assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_2075855 (CHEMBL4731389) Agonist activity at human mu opioid receptor assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_2101679 (CHEMBL4810075) Agonist activity at human GPR139 expressed in HEK293 cells assessed as forskolin-induced cAMP production
- ChEMBL_219007 (CHEMBL818775) Effective concentration of compound against forskolin-stimulated cAMP formation in BHK cells expressing mGluR2 receptor
- ChEMBL_219010 (CHEMBL818778) Effective concentration of compound against forskolin-stimulated cAMP formation in BHK cells expressing mGluR4 receptor
- ChEMBL_219015 (CHEMBL818783) Effective concentration of compound against forskolin-stimulated cAMP formation in BHK cells expressing mGluR2 receptor
- ChEMBL_33362 (CHEMBL644766) Alpha-2B adrenergic receptor agonistic potency as inhibition of forskolin-stimulated cyclic adenosine monophosphate synthesis
- ChEMBL_33656 (CHEMBL649237) Alpha-2C adrenergic receptor agonistic potency as inhibition of forskolin-stimulated cyclic adenosine monophosphate synthesis
- ChEMBL_33794 (CHEMBL648119) Alpha-2A adrenergic receptor agonism measured as ability to inhibit forskolin-stimulated synthesis of cAMP
- ChEMBL_33803 (CHEMBL858095) Alpha-2B adrenergic receptor agonism measured as ability to inhibit forskolin-stimulated synthesis of cAMP
- ChEMBL_33804 (CHEMBL647188) Alpha-2B adrenergic receptor agonism measured as ability to inhibit forskolin-stimulated synthesis of cAMP
- ChEMBL_33811 (CHEMBL647195) Alpha-2C adrenergic receptor agonism measured as ability to inhibit forskolin-stimulated synthesis of cAMP
- ChEMBL_515052 (CHEMBL1034794) Inhibition of human liver glycogen phosphorylase A in HepG2 cells assessed as forskolin-induced glycogenolysis
- ChEMBL_609 (CHEMBL615478) EC50 for inhibition of 50 uM forskolin-stimulated cAMP accumulation against 5-hydroxytryptamine 1A receptor
- ChEBML_1689996 Antagonist activity at recombinant human MOR expressed in HEK293T cells assessed as reduction in DAMGO-induced inhibition of forskolin-stimulated cAMP level preincubated for 15 to 20 mins followed by DAMGO and forskolin addition by GloSensor assay
- ChEMBL_1772778 (CHEMBL4229770) Agonist activity at rat GPR81 expressed in T-REx-CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation preincubated for 30 mins followed by forskolin stimulation and measured after 20 mins by TR-FRET assay
- ChEMBL_1772779 (CHEMBL4229771) Agonist activity at rat GPR109A expressed in T-REx-CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation preincubated for 30 mins followed by forskolin stimulation and measured after 20 mins by TR-FRET assay
- ChEMBL_1843334 (CHEMBL4343761) Agonist activity at human N-terminal FLAG-tagged GPR84 receptor stably expressed in CHO cells assessed as inhibition of forskolin-induced cAMP production preincubated for 20 mins followed by forskolin addition and measured after 20 mins
- ChEMBL_1855660 (CHEMBL4356389) Agonist activity at mouse H3R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by luciferase reporter gene assay
- ChEMBL_1855661 (CHEMBL4356390) Agonist activity at mouse H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by luciferase reporter gene assay
- ChEMBL_2090986 (CHEMBL4772249) Agonist activity at human mu-opioid receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 15 mins followed by forskolin addition in presence of IBMX by Glo-sensor cAMP assay
- ChEMBL_2090987 (CHEMBL4772250) Agonist activity at human delta-opioid receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 15 mins followed by forskolin addition in presence of IBMX by Glo-sensor cAMP assay
- ChEMBL_2090988 (CHEMBL4772251) Agonist activity at human kappa opioid receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 15 mins followed by forskolin addition in presence of IBMX by Glo-sensor cAMP assay
- ChEMBL_2186035 (CHEMBL5098117) Partial agonist activity at CBR2 V1133.32M mutant (unknown origin) stably expressed in HEK293T assessed as decrease in forskolin induced cAMP level preincubated for 15 mins followed by forskolin addition incubated for 15 mins by HTRF assay
- ChEMBL_2186041 (CHEMBL5098123) Partial agonist activity at CBR2 V361.35M mutant (unknown origin) stably expressed in HEK293T assessed as decrease in forskolin induced cAMP level preincubated for 15 mins followed by forskolin addition incubated for 15 mins by HTRF assay
- ChEMBL_2186048 (CHEMBL5098130) Partial agonist activity at CBR2 A2827.36M mutant (unknown origin) stably expressed in HEK293T assessed as decrease in forskolin induced cAMP level preincubated for 15 mins followed by forskolin addition incubated for 15 mins by HTRF assay
- ChEMBL_2186054 (CHEMBL5098136) Partial agonist activity at CBR2 S2857.39L mutant (unknown origin) stably expressed in HEK293T assessed as decrease in forskolin induced cAMP level preincubated for 15 mins followed by forskolin addition incubated for 15 mins by HTRF assay
- ChEMBL_1689996 (CHEMBL4040566) Antagonist activity at recombinant human MOR expressed in HEK293T cells assessed as reduction in DAMGO-induced inhibition of forskolin-stimulated cAMP level preincubated for 15 to 20 mins followed by DAMGO and forskolin addition by GloSensor assay
- ChEMBL_1738556 (CHEMBL4154306) Agonist activity at human H3R expressed in HEK293 cells harboring glosensor-22F cAMP plasmid DNA assessed as inhibition of forskolin-stimulated cAMP accumulation preincubated with cells followed by forskolin addition measured after 10 mins by luminescence assay
- ChEMBL_1738627 (CHEMBL4154377) Agonist activity at human GPR109a expressed in HEK293 cells harboring glosensor-22F cAMP plasmid DNA assessed as inhibition of forskolin-stimulated cAMP accumulation preincubated with cells followed by forskolin addition measured after 10 mins by luminescence assay
- ChEMBL_1843878 (CHEMBL4344305) Agonist activity at human D2S receptor expressed in CHOK1 cells assessed as inhibition of forskolin induced cAMP production preincubated for 10 mins followed by forskolin addition and measured after 5 mins by HTRF assay relative to control
- ChEMBL_1855646 (CHEMBL4356375) Agonist activity at human H3R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by CRE-luciferase reporter gene assay
- ChEMBL_1855652 (CHEMBL4356381) Agonist activity at human H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by CRE-luciferase reporter gene assay
- ChEMBL_2080395 (CHEMBL4736186) Agonist activity at human D2R isoform 2 stably expressed in HEK293 cells assessed as inhibition of forskolin-induced increase of cAMP accumulation preincubated for 10 mins followed by forskolin addition by Glosensor cAMP reagent based luminescence assay
- ChEMBL_2147331 (CHEMBL5031677) Agonist activity at human A3 adenosine receptor expressed in CHO cells assessed as inhibition of forskolin stimulated cAMP formation incubated for 45 mins followed by forskolin stimulation and measured after 15 mins by liquid scintillation spectrometry analysis
- ChEMBL_2186029 (CHEMBL5098111) Partial agonist activity at wild type CB2 receptor (unknown origin) stably expressed in HEK293T assessed as decrease in forskolin induced cAMP level preincubated for 15 mins followed by forskolin addition incubated for 15 mins by HTRF assay
- ChEBML_1668 Inhibition of Forskolin-stimulated adenylate cyclase activity against 5-hydroxytryptamine 1D receptor of guinea pig substantia nigra
- ChEMBL_105899 (CHEMBL717760) Inhibition of forskolin stimulated cAMP production in RGT cells expressing recombinant human Metabotropic glutamate receptor 2
- ChEMBL_106372 (CHEMBL718738) Inhibition of forskolin stimulated cAMP production in RGT cells expressing recombinant human Metabotropic glutamate receptor 3
- ChEMBL_106551 (CHEMBL715496) Compound was evaluated for the inhibition of forskolin stimulated cAMP accumulation in CHO cells expressing hmGluR4a
- ChEMBL_140788 (CHEMBL752777) Agonist potency for the inhibition of Forskolin stimulated cAMP synthesis in cells expressing cloned Y2 receptors
- ChEMBL_140792 (CHEMBL752931) Agonist potency for the inhibition of Forskolin stimulated cAMP synthesis in cells expressing cloned Y2 receptors
- ChEMBL_140793 (CHEMBL752932) Agonist potency for the inhibition of Forskolin stimulated cAMP synthesis in cells expressing cloned Y4 receptors
- ChEMBL_140801 (CHEMBL752048) Agonist potency for the inhibition of Forskolin stimulated cAMP synthesis in cells expressing cloned Y5 receptors
- ChEMBL_1595 (CHEMBL617005) Agonist activity at 5-hydroxytryptamine 1B receptor by measuring the inhibition of forskolin-stimulated cAMP formation
- ChEMBL_1767 (CHEMBL616552) Inhibition of Forskolin-stimulated adenylate cyclase activity against 5-hydroxytryptamine 1B receptor of rat substantia nigra
- ChEMBL_1768 (CHEMBL832876) Inhibition of Forskolin-stimulated adenylate cyclase activity against 5-hydroxytryptamine 1B receptor of rat substantia nigra
- ChEMBL_219039 (CHEMBL821295) Compound was evaluated for the inhibition of forskolin stimulated cAMP accumulation in CHO cells expressing hmGluR7b
- ChEMBL_2296164 Agonist activity at human mGluR7b expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation
- ChEMBL_290 (CHEMBL615728) Effect on forskolin stimulated adenylate cyclase activity at 5-HT1D receptor of guinea pig substantia nigra.
- ChEMBL_310479 (CHEMBL834423) Inhibition of forskolin-induced increase in cAMP in human 5-hydroxytryptamine 1A receptor expressed HeLa cells
- ChEMBL_312909 (CHEMBL826591) Inhibition of (10 uM) forskolin-mediated cAMP production in CHO cells expressing human Adenosine A3 receptor
- ChEMBL_313611 (CHEMBL835018) Inhibition of forskolin stimulated cAMP levels in CHO cell membranes expressing the human NOP receptor (CHOhNOP)
- ChEMBL_330004 (CHEMBL858881) Agonistic activity at human 5HT1A in CHO cells by the inhibition of forskolin-stimulated cAMP accumulation
- ChEMBL_33194 (CHEMBL642221) Alpha-2A adrenergic receptor agonistic potency as inhibition of forskolin-stimulated synthesis of cyclic adenosine monophosphate
- ChEMBL_344944 (CHEMBL870723) Agonist activity at human NPY4 receptor in HEK293 cells by inhibition of forskolin-stimulated cAMP synthesis
- ChEMBL_344945 (CHEMBL868919) Agonist activity at human NPY1 receptor in CHO cells by inhibition of forskolin-stimulated cAMP synthesis
- ChEMBL_344947 (CHEMBL868921) Agonist activity at human NPY2 receptor in CHO cells by inhibition of forskolin-stimulated cAMP synthesis
- ChEMBL_473831 (CHEMBL936826) Activity at human wild type CFTR expressed in CHO cells assessed as forskolin stimulated iodide flux
- ChEMBL_473832 (CHEMBL939224) Activity at human wild type CFTR expressed in Calu3 cells assessed as forskolin stimulated iodide flux
- ChEMBL_514030 (CHEMBL976345) Agonist activity at human CB2 receptor assessed as inhibition of forskolin-induced increase in intracellular cAMP
- ChEMBL_514032 (CHEMBL976347) Agonist activity at human CB1 receptor assessed as inhibition of forskolin-induced increase in intracellular cAMP
- ChEMBL_593 (CHEMBL615463) Effect on forskolin stimulated adenylate cyclase activity at 5-hydroxytryptamine 1A receptor of guinea pig hippocampus.
- ChEMBL_604 (CHEMBL615473) Inhibition of Forskolin-stimulated adenylate cyclase activity against 5-hydroxytryptamine 1A receptor of guinea pig hippocampus
- ChEMBL_674 (CHEMBL615750) Agonistic activity of compound towards 5-hydroxytryptamine 1A receptor was evaluated by forskolin stimulated cAMP assay
- ChEMBL_869 (CHEMBL615924) Inhibition of forskolin-activated adenylate cyclase (cAMP) activity at 5-hydroxytryptamine 1A receptor in rat hippocampus
- ChEMBL_870 (CHEMBL615925) Inhibition of forskolin-activated adenylate cyclase (cAMP) activity at 5-hydroxytryptamine 1A receptor in rat hippocampus
- ChEMBL_873137 (CHEMBL2182986) Agonist activity at mGLUR3 in rat cerebral cortex assessed as inhibition of forskolin-stimulated cAMP production
- ChEMBL_873324 (CHEMBL2185177) Agonist activity at mGLUR2 in rat cerebral cortex assessed as inhibition of forskolin-stimulated cAMP production
- ChEMBL_977441 (CHEMBL2422476) Inhibition of WNT3A signaling in HEK293 cells by luciferase reporter gene assay in presence of forskolin
- ChEMBL_978571 (CHEMBL2421941) Agonist activity at CB2 receptor in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation
- ChEMBL_1843331 (CHEMBL4343758) Agonist activity at recombinant human GPR84 stably expressed in HEK293 cells co-expressing Galpha16 assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 30 mins followed by forskolin addition and measured after 30 mins by HTRF assay
- ChEMBL_2112107 (CHEMBL4820957) Antagonist activity at human AM2 expressed in 1321N1 cells assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 30 mins followed by forskolin addition in presence of IBMX and measured after 15 mins by TR-FRET assay
- ChEMBL_2195578 (CHEMBL5108094) Agonist activity at FLAG-tagged mouse delta opioid receptor expressed in human HEK293 cells assessed as inhibition of forskolin stimulated cAMP production preincubated for 20 mins followed by forskolin stimulation and measured after 15 mins by GloSensor assay
- ChEMBL_2195581 (CHEMBL5108097) Agonist activity at FLAG-tagged mouse mu opioid receptor expressed in human HEK293 cells assessed as inhibition of forskolin stimulated cAMP production preincubated for 20 mins followed by forskolin stimulation and measured after 15 mins by GloSensor assay
- ChEBML_61299 D2 receptor functional activity was measured through reversal of quinpirole inhibited, forskolin stimulated cAMP production from whole cells
- ChEMBL_104624 (CHEMBL872629) Metabotropic glutamate receptor 8 agonist activity against forskolin stimulated c-AMP formation in rat non neuronal cells
- ChEMBL_106201 (CHEMBL713191) Metabotropic glutamate receptor 2 agonist activity against forskolin stimulated c-AMP formation in rat non neuronal cells
- ChEMBL_106395 (CHEMBL717250) Metabotropic glutamate receptor 3 agonist activity against forskolin stimulated c-AMP formation in rat non neuronal cells
- ChEMBL_139108 (CHEMBL749232) Reversal of forskolin-stimulated accumulation of adenylate cyclase in transfected CHO cells, against Muscarinic acetylcholine receptor M4
- ChEMBL_139619 (CHEMBL744784) Reversal of forskolin-stimulated accumulation of adenylate cyclase in transfected CHO cells, against Muscarinic acetylcholine receptor M2
- ChEMBL_145691 (CHEMBL753317) Inhibition of forskolin-stimulated adenylyl cyclase activity by compound in CHO cells expressing Opioid receptor kappa 1
- ChEMBL_146754 (CHEMBL753334) Inhibitory activity against forskolin-stimulated cAMP production in chinese hamster ovary cells expressing Opioid receptor delta 1
- ChEMBL_1568364 (CHEMBL3789017) Agonist activity at human mGlu7 receptor expressed in HEK293 assessed as inhibition of forskolin stimulated cAMP production
- ChEMBL_1672 (CHEMBL616663) Ability to inhibit forskolin-stimulated adenylate cyclase in a cell line expressing human 5-hydroxytryptamine 1D receptor
- ChEMBL_1978551 (CHEMBL4611686) Agonist activity at human GPR81 overexpressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production
- ChEMBL_1984141 (CHEMBL4617547) Agonist activity at human APJ expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production
- ChEMBL_2264531 Antagonist activity at human CB2R expressed in CHO cells assessed as inhibition of forskolin stimulated cyclic AMP production
- ChEMBL_2264532 Antagonist activity at rat CB1R expressed in CHO cells assessed as inhibition of forskolin stimulated cyclic AMP production
- ChEMBL_2443859 Potentiation of CFTR N1303K mutant (unknown origin) transfected in mouse FRT cells in presence of forskolin/VX-770
- ChEMBL_2443860 Potentiation of CFTR W1282X mutant (unknown origin) transfected in mouse FRT cells in presence of forskolin/VX-770
- ChEMBL_31552 (CHEMBL644503) Inhibition of forskolin-stimulated cAMP accumulation in chinese hamster ovary cell membranes expressing human adenosine A3 receptor
- ChEMBL_473833 (CHEMBL939223) Activity at human wild type CFTR expressed in F508del-CF1 cells assessed as forskolin stimulated iodide flux
- ChEMBL_515066 (CHEMBL1034808) Inhibition of liver glycogen phosphorylase A in human HepG2 cells assessed as inhibition of forskolin-induced glucogenolysis
- ChEMBL_61300 (CHEMBL673093) Dopamine receptor D2 functional activity was assessed via inhibition of forskolin stimulated cAMP production from Whole cells
- ChEMBL_617 (CHEMBL615167) In vitro potency at human 5-hydroxytryptamine 1A receptor in inhibiting forskolin-stimulated accumulation of intracellular cAMP
- ChEMBL_62963 (CHEMBL677454) Dopamine D2 receptor functional activity was assessed via inhibition of forskolin stimulated cAMP production from Whole cells
- ChEMBL_662218 (CHEMBL1252310) Agonist activity at mu opioid expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation
- ChEMBL_662219 (CHEMBL1252311) Agonist activity at delta opioid expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation
- ChEMBL_757478 (CHEMBL1804450) Inhibition of human CB2 receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production
- ChEMBL_83662 (CHEMBL694891) Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H3 receptor
- ChEMBL_87224 (CHEMBL696445) Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor
- ChEMBL_873135 (CHEMBL2182984) Agonist activity at human mGLUR3 assessed as inhibition of forskolin-stimulated cAMP production by cell-based assay
- ChEMBL_873136 (CHEMBL2182985) Agonist activity at human mGLUR2 assessed as inhibition of forskolin-stimulated cAMP production by cell-based assay
- ChEMBL_1654441 (CHEMBL4003807) Antagonist activity at human adenosine A2A receptor expressed in HEK293 cell membranes assessed as decrease in CGS-21680/forskolin-induced cAMP level pretreated for 15 mins followed by agonist addition for 30 mins and subsequent forskolin stimulation measured after 30 mins
- ChEMBL_1654447 (CHEMBL4003813) Antagonist activity at human adenosine A1 receptor expressed in HEK293 cell membranes assessed as decrease in CPA/forskolin-induced cAMP level pretreated for 15 mins followed by agonist addition for 15 mins and subsequent forskolin stimulation measured after 30 mins
- ChEMBL_1654448 (CHEMBL4003814) Antagonist activity at human adenosine A2B receptor expressed in HEK293 cell membranes assessed as decrease in NECA/forskolin-induced cAMP level pretreated for 15 mins followed by agonist addition for 15 mins and subsequent forskolin stimulation measured after 30 mins
- ChEMBL_1747163 (CHEMBL4181673) Antagonist activity at human CRTh2 expressed in HEK cells assessed as inhibition of DK-PGD2-mediated attenuation of forskolin-induced cAMP accumulation preincubated for 10 mins followed by forskolin stimulation measured after 10 to 60 mins by ELISA based chemiluminescence assay
- ChEMBL_1843329 (CHEMBL4343756) Agonist activity at recombinant human GPR84 expressed in CHO cell membranes co-expressing beta-arrestin2 assessed as inhibition of forskolin-stimulated [3H]cAMP accumulation preincubated for 5 mins followed by forskolin stimulation and measured after 15 mins by radioactive assay
- ChEMBL_1930363 (CHEMBL4433614) Antagonist activity at human delta opioid receptor expressed in CHO cells assessed as reduction in SNC80-induced inhibition of forskolin stimulated cAMP accumulation preincubated for 15 mins followed SNC80 and forskolin addition and measured after 10 mins relative to control
- ChEMBL_2106125 (CHEMBL4814800) Agonist activity at mouse HA-tagged mu opioid receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP production preincubated for 20 mins followed by forskolin stimulation and measured after 15 mins by Glo-sensor reagent based assay
- ChEMBL_2106126 (CHEMBL4814801) Agonist activity at mouse FLAG-tagged kappa opioid receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP production preincubated for 20 mins followed by forskolin stimulation and measured after 15 mins by Glo-sensor reagent based assay
- ChEMBL_2106127 (CHEMBL4814802) Agonist activity at mouse FLAG-tagged delta opioid receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP production preincubated for 20 mins followed by forskolin stimulation and measured after 15 mins by Glo-sensor reagent based assay
- ChEMBL_2150927 (CHEMBL5035389) Agonist activity at MOR (unknown origin) expressed in HEK293-A cells assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 30 mins in presence of 3-isobutyl-1-methylxanthine followed by forskolin and compound addition by competitive PKA binding assay
- ChEMBL_2150929 (CHEMBL5035391) Agonist activity at KOR (unknown origin) expressed in HEK293-A cells assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 30 mins in presence of 3-isobutyl-1-methylxanthine followed by forskolin and compound addition by competitive PKA binding assay
- ChEMBL_2150931 (CHEMBL5035393) Agonist activity at DOR (unknown origin) expressed in HEK293-A cells assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 30 mins in presence of 3-isobutyl-1-methylxanthine followed by forskolin and compound addition by competitive PKA binding assay
- ChEMBL_2150933 (CHEMBL5035395) Agonist activity at NPFF1R (unknown origin) expressed in HEK293-A cells assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 30 mins in presence of 3-isobutyl-1-methylxanthine followed by forskolin and compound addition by competitive PKA binding assay
- ChEMBL_2150935 (CHEMBL5035397) Agonist activity at NPFF2R (unknown origin) expressed in HEK293-A cells assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 30 mins in presence of 3-isobutyl-1-methylxanthine followed by forskolin and compound addition by competitive PKA binding assay
- ChEMBL_2186031 (CHEMBL5098113) Partial agonist activity at wild type CB2 receptor (unknown origin) stably expressed in HEK293T assessed as decrease in forskolin induced cAMP level preincubated for 15 mins followed by forskolin addition incubated for 15 mins in presence of cannabidiol by HTRF assay
- ChEMBL_2186036 (CHEMBL5098118) Partial agonist activity at CBR2 V1133.32M mutant (unknown origin) stably expressed in HEK293T assessed as decrease in forskolin induced cAMP level preincubated for 15 mins followed by forskolin addition incubated for 15 mins in presence of cannabidiol by HTRF assay
- ChEMBL_2186037 (CHEMBL5098119) Partial agonist activity at CBR2 V1133.32M mutant (unknown origin) stably expressed in HEK293T assessed as decrease in forskolin induced cAMP level preincubated for 15 mins followed by forskolin addition incubated for 15 mins in presence of JWH-133 by HTRF assay
- ChEMBL_2186042 (CHEMBL5098124) Partial agonist activity at CBR2 V361.35M mutant (unknown origin) stably expressed in HEK293T assessed as decrease in forskolin induced cAMP level preincubated for 15 mins followed by forskolin addition incubated for 15 mins in presence of cannabidiol by HTRF assay
- ChEMBL_2186043 (CHEMBL5098125) Partial agonist activity at CBR2 V361.35M mutant (unknown origin) stably expressed in HEK293T assessed as decrease in forskolin induced cAMP level preincubated for 15 mins followed by forskolin addition incubated for 15 mins in presence of JWH-133 by HTRF assay
- ChEMBL_2186049 (CHEMBL5098131) Partial agonist activity at CBR2 A2827.36M mutant (unknown origin) stably expressed in HEK293T assessed as decrease in forskolin induced cAMP level preincubated for 15 mins followed by forskolin addition incubated for 15 mins in presence of cannabidiol by HTRF assay
- ChEMBL_2186050 (CHEMBL5098132) Partial agonist activity at CBR2 A2827.36M mutant (unknown origin) stably expressed in HEK293T assessed as decrease in forskolin induced cAMP level preincubated for 15 mins followed by forskolin addition incubated for 15 mins in presence of JWH-133 by HTRF assay
- ChEMBL_2186055 (CHEMBL5098137) Partial agonist activity at CBR2 S2857.39L mutant (unknown origin) stably expressed in HEK293T assessed as decrease in forskolin induced cAMP level preincubated for 15 mins followed by forskolin addition incubated for 15 mins in presence of cannabidiol by HTRF assay
- ChEMBL_2186056 (CHEMBL5098138) Partial agonist activity at CBR2 S2857.39L mutant (unknown origin) stably expressed in HEK293T assessed as decrease in forskolin induced cAMP level preincubated for 15 mins followed by forskolin addition incubated for 15 mins in presence of JWH-133 by HTRF assay
- ChEMBL_2296131 Agonist activity at human mGluR7 expressed in CHO cells expressing CRE-Luc reporter gene assessed as reduction in forskolin-stimulated cAMP production preincubated for 15 mins followed by forskolin stimulation and measured after 5 hrs by luminescence based Steady glo assay
- ChEMBL_2296160 Agonist activity at mouse mGluR7 expressed in CHO cells expressing CRE-Luc reporter gene assessed as reduction in forskolin-stimulated cAMP production preincubated for 15 mins followed by forskolin stimulation and measured after 5 hrs by luminescence based Steady glo assay
- ChEMBL_956569 (CHEMBL2380019) Antagonist activity at human adenosine A2a receptor expressed in CHOK1 cells assessed as inhibition of NECA/forskolin-induced cAMP accumulation incubated for 15 mins prior to NECA/forskolin challenge measured after 5.5 to 6 hrs by beta-galactosidase reporter gene assay
- ChEBML_143837 Functional response of NPY to inhibit forskolin-stimulated cAMP in cells stably transfected with Neuropeptide Y receptor type 1
- ChEBML_591 Compound was tested for the inhibition of forskolin-stimulated adenylate cyclase at 5-hydroxytryptamine 1A receptor in guinea pig
- ChEMBL_104463 (CHEMBL713650) Metabotropic glutamate receptor 6 agonist activity to influence forskolin stimulated c-AMP formation in rat non neuronal cells
- ChEMBL_104465 (CHEMBL712211) Metabotropic glutamate receptor 6 antagonist activity to influence forskolin stimulated c-AMP formation in rat non neuronal cells
- ChEMBL_104483 (CHEMBL712464) Metabotropic glutamate receptor 7 agonist activity to influence forskolin stimulated c-AMP formation in rat non neuronal cells
- ChEMBL_104485 (CHEMBL712466) Metabotropic glutamate receptor 7 antagonist activity to influence forskolin stimulated c-AMP formation in rat non neuronal cells
- ChEMBL_104626 (CHEMBL715573) Metabotropic glutamate receptor 8 antagonist activity to influence forskolin stimulated c-AMP formation in rat non neuronal cells
- ChEMBL_106694 (CHEMBL714496) Metabotropic glutamate receptor 4 agonist activity to influence forskolin stimulated c-AMP formation in rat non neuronal cells
- ChEMBL_106696 (CHEMBL713998) Metabotropic glutamate receptor 4 antagonist activity to influence forskolin stimulated c-AMP formation in rat non neuronal cells
- ChEMBL_1351338 (CHEMBL3266187) Agonist activity at rat CB1 receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP level
- ChEMBL_1446274 (CHEMBL3373668) Agonist activity at human CB2 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP production
- ChEMBL_1446275 (CHEMBL3373669) Agonist activity at human CB1 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP production
- ChEMBL_1502620 (CHEMBL3592881) Agonist activity at human CB1 receptor expressed in HEK cells assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_1502621 (CHEMBL3592882) Agonist activity at human CB2 receptor expressed in HEK cells assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_1567255 (CHEMBL3788712) Activation of GPR109A in human A431 cells assessed as suppression of forskolin-induced cAMP production after 30 mins
- ChEMBL_1568363 (CHEMBL3789016) Agonist activity at rat mGlu4 receptor expressed in HEK293 cells assessed as inhibition of forskolin stimulated cAMP production
- ChEMBL_1596 (CHEMBL616623) Inhibition of forskolin-stimulated c-AMP formation by human 5-hydroxytryptamine 1B receptor expressed in CHO-K1 cells
- ChEMBL_1906940 (CHEMBL4409298) Inhibition of full length delta opioid receptor (unknown origin) assessed as inhibition of forskolin-stimulated intracellular cAMP accumulation
- ChEMBL_2158147 (CHEMBL5042897) Agonist activity at human NPFF1 receptor expressed in HEK293 cells assessed as reduction in forskolin- stimulated cAMP level
- ChEMBL_2271478 Potentiation of CFTR F508del mutant in rat FRT cells assessed as increase in iodide influx in presence of forskolin
- ChEMBL_313039 (CHEMBL835730) Inhibitory concentration measured by cellular decreases in forskolin stimulated cAMP in CHO cells stably transfected with hORL1 receptor
- ChEMBL_329335 (CHEMBL863558) Agonistic activity at cloned human 5HT1A receptor expressed in CHO cells by inhibition of forskolin-induced cAMP production
- ChEMBL_410543 (CHEMBL912413) Activity at rat dopamine D2L receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production
- ChEMBL_410545 (CHEMBL912415) Activity at human dopamine D4.4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production
- ChEMBL_439390 (CHEMBL888504) Agonist activity at human SST5 receptor expressed in CHOK1 cells assessed as effect on forskolin-induced cAMP production
- ChEMBL_444829 (CHEMBL895080) Agonistic potency at human adrenergic alpha-2A receptor expressed in CHO cells assessed as forskolin-induced cAMP accumulation
- ChEMBL_444830 (CHEMBL895081) Agonistic potency at human adrenergic alpha-2B receptor expressed in CHO cells assessed as forskolin-induced cAMP accumulation
- ChEMBL_444831 (CHEMBL895082) Agonistic potency at human adrenergic Alpha-2C receptor expressed in CHO cells assessed as forskolin-induced cAMP accumulation
- ChEMBL_444840 (CHEMBL895095) Agonist activity at human CB1 receptor expressed in HEK293 cells assessed as reversal of forskolin-evoked cAMP accumulation
- ChEMBL_444842 (CHEMBL895097) Agonist activity at human CB2 receptor expressed in CHOK1 cells assessed as reversal of forskolin-evoked cAMP accumulation
- ChEMBL_462578 (CHEMBL928504) Agonist activity at human CB2 receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP level
- ChEMBL_465781 (CHEMBL931050) Agonist activity at human adenosine A3 receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP
- ChEMBL_556453 (CHEMBL955670) Agonist activity at mu opioid receptor in human SHSY5Y cells assessed as inhibition of forskolin-stimulated cAMP production
- ChEMBL_560040 (CHEMBL1021560) Agonist activity at human CB2 receptor expressed in CHO cells assessed as suppression of forskolin-stimulated cAMP accumulation
- ChEMBL_560042 (CHEMBL1022429) Agonist activity at human CB1 receptor expressed in CHO cells assessed as suppression of forskolin-stimulated cAMP accumulation
- ChEMBL_563064 (CHEMBL1011894) Agonist activity at human recombinant CB1R expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP level
- ChEMBL_563086 (CHEMBL1012779) Agonist activity at rat recombinant CB1R expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP level
- ChEMBL_567040 (CHEMBL1029937) Agonist activity at human CB2 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP release
- ChEMBL_578756 (CHEMBL1052329) Agonist activity at human MT1 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_583691 (CHEMBL1061737) Agonist activity at human recombinant CB2 receptor expressed in CHO cells assessed as inhibition forskolin-induced cAMP release
- ChEMBL_596096 (CHEMBL1040884) Agonist activity at rat CB1 receptor assessed as inhibition of forskolin-induced cAMP production by cell based assay
- ChEMBL_596098 (CHEMBL1040886) Agonist activity at rat CB2 receptor assessed as inhibition of forskolin-induced cAMP production by cell based assay
- ChEMBL_59903 (CHEMBL671809) In vitro inhibition of forskolin-stimulated cAMP accumulation in GH4C1 cells transfected with the human Dopamine D2 receptor
- ChEMBL_605612 (CHEMBL1069216) Agonist activity at human FFA2 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP production
- ChEMBL_61299 (CHEMBL673092) D2 receptor functional activity was measured through reversal of quinpirole inhibited, forskolin stimulated cAMP production from whole cells
- ChEMBL_718725 (CHEMBL1680944) Agonist activity at human recombinant GPR109a expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation
- ChEMBL_740154 (CHEMBL1763214) Agonist activity at human CB2 receptor transfected in CHO cells assessed as inhibition of forskolin-stimulated cAMP production
- ChEMBL_740155 (CHEMBL1763215) Agonist activity at human CB1 receptor transfected in CHO cells assessed as inhibition of forskolin-stimulated cAMP production
- ChEMBL_757135 (CHEMBL1803275) Agonist activity at human recombinant CB2 receptor expressed in CHO cells assessed as inhibition forskolin-induced cAMP release
- ChEMBL_820673 (CHEMBL2037944) Agonist activity at human recombinant GPR109a expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_960429 (CHEMBL2389080) Agonist activity at human CB2 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP production
- ChEMBL_971498 (CHEMBL2406560) Antagonist activity at human MCHR1 expressed in CHOK1 cells assessed as inhibition of MCH/forskolin-stimulated cAMP production
- ChEMBL_1764477 (CHEMBL4199724) Antagonist activity at human CRTh2 expressed in HEK cells assessed as inhibition of DK-PGD2-mediated attenuation of forskolin-induced intracellular cAMP accumulation preincubated for 10 mins followed by forskolin stimulation measured after 10 to 60 mins by ELISA based chemiluminescence assay
- ChEMBL_2057190 (CHEMBL4712191) Antagonist activity at human GPR84 expressed in CHO-K1 cells assessed as reduction in 6-OAU-induced inhibition of forskolin stimulated cAMP production preincubated for 15 mins followed by forskolin and 6-OAU stimulation and measured after 30 mins by luminescence method
- ChEMBL_2112109 (CHEMBL4820959) Antagonist activity at human CGRP-2 (8 to 37 residues) expressed in 1321N1 cells assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 30 mins followed by forskolin addition in presence of IBMX and measured after 15 mins by TR-FRET assay
- ChEMBL_2186032 (CHEMBL5098114) Partial agonist activity at wild type CB2 receptor (unknown origin) stably expressed in HEK293T assessed as decrease in forskolin induced cAMP level preincubated for 15 mins followed by forskolin addition incubated for 15 mins in presence of JWH-133 by HTRF assay
- ChEMBL_956568 (CHEMBL2380018) Antagonist activity at human adenosine A1 receptor expressed in CHOK1 cells assessed as inhibition of R-PIA/forskolin-induced cAMP accumulation incubated for 15 mins prior to R-PIA/forskolin challenge measured after 5.5 to 6 hrs by beta-galactosidase reporter gene assay
- ChEBML_144137 Functional response of NPY to inhibit forskolin-stimulated cAMP in cells stably transfected with rat Neuropeptide Y receptor type 5
- ChEMBL_1008 (CHEMBL616051) In vitro effective concentration required to inhibit forskolin-stimulated cAMP levels in HA7 cells expressing human 5-HT1A receptor
- ChEMBL_1334662 (CHEMBL3239862) Agonist activity at rat CB2 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_1499462 (CHEMBL3583995) Agonist activity at human CB2 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-stimulated cAMP production
- ChEMBL_1499463 (CHEMBL3583996) Agonist activity at human CB1 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-stimulated cAMP production
- ChEMBL_1779341 (CHEMBL4236333) Agonist activity at recombinant rat Cb1 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP levels
- ChEMBL_1779342 (CHEMBL4236334) Agonist activity at recombinant human Cb2 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP levels
- ChEMBL_2090959 (CHEMBL4772222) Agonist activity at human NPFFR2 expressed in CHO cells assessed as inhibition of forskolin-induced intracellular cyclic cAMP accumulation
- ChEMBL_2214476 (CHEMBL5127608) Agonist activity at human MOP stably expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_2349877 Binding affinity to human KOR expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation by HTRF assay
- ChEMBL_310710 (CHEMBL838055) Agonist activity towards human Melatonin receptor type 1A was determined by its ability to inhibit forskolin stimulated cAMP accumulation
- ChEMBL_310711 (CHEMBL838056) Agonist activity towards human Melatonin receptor type 1B was determined by its ability to inhibit forskolin stimulated cAMP accumulation
- ChEMBL_422611 (CHEMBL911647) Inverse agonist activity at human recombinant CB1 receptor assessed as inhibition of forskolin-induced cAMP production in CHO cells
- ChEMBL_450740 (CHEMBL901023) Antagonist activity at human adenosine A3 receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation
- ChEMBL_465959 (CHEMBL947083) Antagonist activity at rat mGluR2 assessed as effect on (1S,3R)-ACPD-induced inhibition of forskolin-stimulated cAMP production
- ChEMBL_467325 (CHEMBL939920) Agonist activity at human dopamine D2 receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production
- ChEMBL_476981 (CHEMBL937486) Antagonist activity at rat mGluR2 assessed as blockade of (1S,3R)-ACPD-induced inhibition of forskolin-stimulated cAMP production
- ChEMBL_491655 (CHEMBL946373) Inhibition of human glycogen phosphorylase alpha in HepG2 cells assessed as inhibition of forskolin-induced glycogenolysis after 60 mins
- ChEMBL_536713 (CHEMBL995104) Intrinsic activity at human D3 receptor expressed in CHO dhfr- cells assessed as inhibition of forskolin-induced cAMP release
- ChEMBL_563053 (CHEMBL1011883) Inverse agonist activity at human recombinant CB1R expressed in CHO cells assessed as increase in forskolin-stimulated cAMP level
- ChEMBL_596092 (CHEMBL1040880) Agonist activity at human recombinant CB1 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP production
- ChEMBL_596094 (CHEMBL1040882) Agonist activity at human recombinant CB2 receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP production
- ChEMBL_603083 (CHEMBL1038805) Agonist activity at human 5HT1A receptor expressed in CHO cells assessed as inhibition of forskolin-induced adenylate cyclase activity
- ChEMBL_633683 (CHEMBL1118308) Agonist activity at GPR109a expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production by HTRF assay
- ChEMBL_661796 (CHEMBL1252112) Agonist activity at mGlu2 receptor expressed in CHO cells assessed as inhibition of forskolin-induced increase of cAMP level
- ChEMBL_661798 (CHEMBL1252114) Agonist activity at mGlu4 receptor expressed in CHO cells assessed as inhibition of forskolin-induced increase of cAMP level
- ChEMBL_686569 (CHEMBL1292356) Binding affinity at mGluR2 expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production after 10 mins
- ChEMBL_686570 (CHEMBL1292357) Binding affinity at mGluR4 expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production after 10 mins
- ChEMBL_701233 (CHEMBL1648853) Agonist activity at human cloned CB1 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_701235 (CHEMBL1648855) Agonist activity at human cloned CB2 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_741484 (CHEMBL1763799) Agonist activity at human CB2 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-stimulated cAMP level
- ChEMBL_741486 (CHEMBL1763801) Agonist activity at human CB1 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-stimulated cAMP level
- ChEMBL_741489 (CHEMBL1763804) Agonist activity at rat CB2 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-stimulated cAMP level
- ChEMBL_741491 (CHEMBL1763806) Agonist activity at rat CB1 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-stimulated cAMP level
- ChEMBL_824764 (CHEMBL2044691) Agonist activity at human adenosine A1 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_824765 (CHEMBL2044692) Agonist activity at human adenosine A3 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_851501 (CHEMBL2156617) Inverse agonist activity against CB1 receptor expressed in human CHO cells assessed as effect on forskolin-stimulated cAMP level
- ChEMBL_1620882 (CHEMBL3863165) Inverse agonist activity at human cannabinoid receptor 1 expressed in HEK293 cells assessed as increase in forskolin-induced cAMP production preincubated with cells followed by forskolin stimulation measured after 30 mins in presence of D2-labeled cAMP and Eu3+-TBP-NHS cryptate by HTRF assay
- ChEMBL_1620883 (CHEMBL3863166) Inverse agonist activity at human cannabinoid receptor 2 expressed in HEK293 cells assessed as increase in forskolin-induced cAMP production preincubated with cells followed by forskolin stimulation measured after 30 mins in presence of D2-labeled cAMP and Eu3+-TBP-NHS cryptate by HTRF assay
- ChEMBL_1892882 (CHEMBL4394803) Antagonist activity at recombinant human Gi-coupled H3 receptor expressed in HEK293T cells assessed as reduction in histamine-induced inhibition of forskolin-stimulated cAMP accumulation preincubated for 10 to 15 followed by histamine addition for 10 mins and subsequent forskolin-stimulation by Glo-sensor assay
- ChEMBL_1913799 (CHEMBL4416382) Allosteric modulator activity at human CB1 receptor expressed in HEK293 cells assessed as suppression of 100 nM CP55,940-induced reduction in forskolin-stimulated cAMP production pre-incubated for 25 mins before forskolin and CP55,940 addition and measured after 22 mins by BRET CAMYEL cAMP assay
- ChEMBL_2144958 (CHEMBL5029238) Positive allosteric modulation of human D2 receptor expressed in CHO cells assessed as inhibition of forskolin stimulated cAMP accumulation by measuring dopamine EC50 at 0.01 nM pretreated for 10 mins followed by forskolin stimulation for 5 mins and measured after 1 hr by HTRF assay
- ChEMBL_2152682 (CHEMBL5037229) Antagonist activity at human adenosine A1 receptor expressed in Flp-In-CHO cell membrane assessed as inhibition of forskolin-stimulated cAMP accumulation preincubated for 10 min followed by forskolin stimulation and further incubated for 30 mins in presence of NECA by alphascreen LANCE assay
- ChEMBL_2186097 (CHEMBL5098179) Positive allosteric modulation of JWH-133-induced agonist activity at wild type CB2 receptor (unknown origin) stably expressed in HEK293T cells assessed as inhibition of forskolin-stimulated cAMP levels preincubated for 15 mins followed by forskolin addition incubated for 15 mins by HTRF assay
- ChEMBL_2326235 Antagonist activity at human A1R expressed in Flp-In-CHO cells assessed as NECA-mediated reduction in forskolin-stimulated cAMP accumulation by measuring equilibrium dissociation constant incubated for 30 to 60 mins followed by forskolin/NECA addition measured after 30 mins by LANCE cAMP assay
- ChEBML_1651 Compound was tested for the inhibition of forskolin-stimulated adenylate cyclase at 5-hydroxytryptamine 1D receptor in guinea pig substantia nigra
- ChEMBL_106057 (CHEMBL718223) Agonist activity against Metabotropic glutamate receptor 2 expressed in CHO cells was evaluated by measuring forskolin-induced cyclic AMP formation
- ChEMBL_106061 (CHEMBL718226) Antagonist activity against Metabotropic glutamate receptor 2 expressed in CHO cells was evaluated by measuring forskolin-induced cyclic AMP formation
- ChEMBL_1291356 (CHEMBL3117772) Agonist activity at human CB1 receptor expressed in CHO-K1 cells assessed as forskolin-induced cAMP accumulation after 1 hr
- ChEMBL_1291359 (CHEMBL3117775) Agonist activity at human CB2 receptor expressed in CHO-K1 cells assessed as forskolin-induced cAMP accumulation after 1 hr
- ChEMBL_1334663 (CHEMBL3239863) Inverse agonist activity at rat CB2 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_1352251 (CHEMBL3268969) Agonist activity at MT1 receptor (unknown origin) expressed in mouse NIH3T3 cells assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_1352252 (CHEMBL3268970) Agonist activity at MT2 receptor (unknown origin) expressed in mouse NIH3T3 cells assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_1353 (CHEMBL616538) Ability to inhibit the forskolin-stimulated c-AMP formation mediated by human 5-hydroxytryptamine 1B receptor in CHO-K1 cells
- ChEMBL_143299 (CHEMBL753002) Tested in a cellular assay measuring forskolin-induced cyclic AMP accumulation in 293 cells transfected with the human NPY5 receptor
- ChEMBL_1454507 (CHEMBL3365949) Agonist activity at human recombinant A3AR expressed in CHO cells assessed as inhibition of forskolin-induced stimulation of cAMP production
- ChEMBL_1464105 (CHEMBL3406631) Inhibition of human CB1 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins
- ChEMBL_1464106 (CHEMBL3406632) Inhibition of human CB2 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins
- ChEMBL_1522819 (CHEMBL3630378) Agonist activity at mu opioid receptor (unknown origin) transfected in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production
- ChEMBL_1723 (CHEMBL616928) Ability to inhibit the forskolin-stimulated c-AMP formation mediated by human 5-hydroxytryptamine 1D receptor in CHO-K1 cells
- ChEMBL_1778263 (CHEMBL4235255) Inhibition of CFTR-mediated forskolin-induced chloride secretion in human T84 cells after 15 mins by short-circuit current analysis
- ChEMBL_1870470 (CHEMBL4371637) Agonist activity at GPR109A (unknown origin) assessed as inhibition of forskolin-mediated cAMP accumulation after 15 mins by fluorescence assay
- ChEMBL_1927467 (CHEMBL4430539) Agonist activity at human MOR expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation by EIA method
- ChEMBL_1927482 (CHEMBL4430554) Agonist activity at human DOR expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation by EIA method
- ChEMBL_2018516 (CHEMBL4672094) Agonist activity at human MOR expressed in CHOK1 cells assessed as inhibition of forskolin-stimulated cAMP accumulation by FRET assay
- ChEMBL_2184577 (CHEMBL5096659) Displacement of 35S]GTPgammaS from human CB2 receptor transfected in CHO cells assessed as inhibition forskolin-stimulated cyclic AMP production
- ChEMBL_2303322 Antagonist activity at CXCR4 receptor in human MIA PaCa-2 cells assessed as reduction in forskolin-stimulated inhibition of cAMP production
- ChEMBL_31394 (CHEMBL643652) Effect on forskolin-stimulated cyclic AMP production in intact chinese hamster ovary (CHO) cell expressing the human Adenosine A3 receptor
- ChEMBL_429551 (CHEMBL918092) Agonist activity at human adrenergic alpha-2A receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_429552 (CHEMBL919550) Agonist activity at human adrenergic Alpha-2C receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_458713 (CHEMBL942048) Agonist activity at human recombinant dopamine D3 receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation
- ChEMBL_468951 (CHEMBL951290) Agonist activity at mouse MC1R expressed in HEK293 cells assessed as forskolin-stimulated response by beta-galactosidase reporter gene assay
- ChEMBL_468952 (CHEMBL951291) Agonist activity at mouse MC3R expressed in HEK293 cells assessed as forskolin-stimulated response by beta-galactosidase reporter gene assay
- ChEMBL_468953 (CHEMBL951292) Agonist activity at mouse MC4R expressed in HEK293 cells assessed as forskolin-stimulated response by beta-galactosidase reporter gene assay
- ChEMBL_468954 (CHEMBL951293) Agonist activity at mouse MC5R expressed in HEK293 cells assessed as forskolin-stimulated response by beta-galactosidase reporter gene assay
- ChEMBL_579667 (CHEMBL1064087) Agonist activity at GPR109a receptor transfected in CHOK1 cells assessed as inhibition of forskolin-induced cAMP generation by HTRF assay
- ChEMBL_579670 (CHEMBL1064090) Agonist activity at GPR109b receptor transfected in CHOK1 cells assessed as inhibition of forskolin-induced cAMP generation by HTRF assay
- ChEMBL_598162 (CHEMBL1044346) Inverse agonist activity at human CB1 receptor assessed as forskolin-induced cAMP level by adenylyl cyclase activation flash plate assay
- ChEMBL_829215 (CHEMBL2060037) Agonist activity at human GPR109a expressed in CHO cells assessed as decrease in forskolin-stimulated cAMP production by HTRF assay
- ChEMBL_859789 (CHEMBL2169617) Agonist activity at human recombinant CB2 receptor expressed in CHO cell membranes assessed as inhibition of forskolin-induced cAMP accumulation
- ChEMBL_873128 (CHEMBL2182977) Antagonist activity at mGLUR3 expressed in CHO cells assessed as inhibition of glutamate-induced inhibition of forskolin-stimulated cAMP production
- ChEMBL_873130 (CHEMBL2182979) Antagonist activity at mGLUR2 expressed in CHO cells assessed as inhibition of glutamate-induced inhibition of forskolin-stimulated cAMP production
- ChEMBL_936 (CHEMBL616301) Ability to inhibit the forskolin-stimulated c-AMP formation mediated by human 5-hydroxytryptamine 1A receptor in CHO-K1 cells
- ChEMBL_952419 (CHEMBL2352785) Agonist activity at human recombinant CB2 receptor expressed in CHO cells assessed as inhibition of forskolin-induced accumulation of cAMP
- ChEMBL_966652 (CHEMBL2399857) Agonist activity at human dopamine D2L receptor expressed in HEK293 cells assessed as forskolin-stimulated cAMP accumulation after 15 mins
- ChEMBL_1296790 (CHEMBL3132473) Partial agonist activity at recombinant human adenosine A3 receptor expressed in CHO cells assessed as stimulation of forskolin-induced intracellular cAMP production preincubated for 45 mins followed by forskolin challenge measured after 15 mins by liquid scintillation counting analysis in presence of rolipram and adenosine deaminase
- ChEMBL_1645467 (CHEMBL3994396) Antagonist activity at human adenosine A1 receptor expressed in HEK293 cells assessed as reduction in CPA-mediated inhibition of forskolin-stimulated cAMP level preincubated for 15 mins followed by CPA treatment for 15 mins and subsequent addition of forskolin measured after 30 mins by HTRF assay
- ChEMBL_106035 (CHEMBL717203) Effect on 1S,3R-ACPD-induced inhibition of forskolin stimulated cyclic-AMP in RGT cells expressing human Metabotropic glutamate receptor 2
- ChEMBL_106376 (CHEMBL718350) Effect on 1S,3R-ACPD-induced inhibition of forskolin stimulated cyclic-AMP in RGT cells expressing human Metabotropic glutamate receptor 3
- ChEMBL_1278918 (CHEMBL3097218) Antagonist activity at CXCR4 (unknown origin) expressed in CHO-K1 cells assessed as inhibition of SDF-1alpha/forskolin-induced cAMP production
- ChEMBL_1285973 (CHEMBL3106280) Agonist activity at rat CB1 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 30 mins
- ChEMBL_1444456 (CHEMBL3373564) Agonist activity at human CB2 receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production after 30 mins
- ChEMBL_1444457 (CHEMBL3373565) Agonist activity at human CB1 receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production after 30 mins
- ChEMBL_1471048 (CHEMBL3420493) Agonist activity at human recombinant adenosine A1 receptor transfected in CHO cells assessed as inhibition of forskolin-induced adenylyl cyclase activity
- ChEMBL_1764515 (CHEMBL4199762) Agonist activity at human CRTh2 expressed in HEK cells assessed as forskolin-induced intracellular cAMP accumulation by ELISA based chemiluminescence assay
- ChEMBL_1829275 (CHEMBL4329149) Agonist activity at S1P1 receptor (unknown origin) assessed as reduction in forskolin-induced cAMP accumulation after 4 hrs by fluorescence assay
- ChEMBL_1905178 (CHEMBL4407536) Inverse agonist activity at human A1AR expressed in CHO cells assessed as increase in forskolin-stimulated cAMP production by alphascreen assay
- ChEMBL_1923906 (CHEMBL4426862) Agonist activity at rat CB1 receptor expressed in HEK293 cells assessed as decrease in forskolin-stimulated cAMP levels after 30 mins
- ChEMBL_1923908 (CHEMBL4426864) Agonist activity at human CB2 receptor expressed in HEK293 cells assessed as decrease in forskolin-stimulated cAMP levels after 30 mins
- ChEMBL_1978133 (CHEMBL4611268) Antagonist activity at Prolink-tagged human CXCR6 receptor assessed as inhibition of forskolin-induced cAMP accumulation by DiscoveRx cell based assay
- ChEMBL_2001 (CHEMBL617094) Compound was tested for intrinsic efficacy against human 5-hydroxytryptamine 1D receptor alpha measured as the reduction of forskolin-stimulated cAMP.
- ChEMBL_2002 (CHEMBL617095) Compound was tested for intrinsic efficacy against human 5-hydroxytryptamine 1D receptor beta measured as the reduction of forskolin-stimulated cAMP.
- ChEMBL_2020 (CHEMBL617315) Compound was tested for intrinsic efficacy against human 5-hydroxytryptamine 1D receptor beta measured as the reduction of forskolin-stimulated cAMP.
- ChEMBL_2033 (CHEMBL616867) Compound was tested for intrinsic efficacy against human 5-hydroxytryptamine 1D receptor beta measured as the reduction of forskolin-stimulated cAMP.
- ChEMBL_2276734 Partial agonist activity at human D2 receptor expressed in CHO-K1 cells assessed as forskolin-stimulated cAMP production incubated for 60 mins
- ChEMBL_313054 (CHEMBL835745) Inhibitory concentration required to reverse the dopamine inhibition of forskolin-stimulated adenyl cyclase activity in CHO cells stably expressing hD4 receptor
- ChEMBL_33088 (CHEMBL643580) Agonistic activity towards human Alpha-2A adrenergic receptor was measured as ability to inhibit forskolin-stimulated synthesis of cyclic adenosine monophosphate
- ChEMBL_33361 (CHEMBL644765) Agonistic activity towards human Alpha-2B adrenergic receptor was measured as ability to inhibit forskolin-stimulated synthesis of cyclic adenosine monophosphate
- ChEMBL_33655 (CHEMBL649236) Agonistic activity towards human Alpha-2C adrenergic receptor was measured as ability to inhibit forskolin-stimulated synthesis of cyclic adenosine monophosphate
- ChEMBL_425919 (CHEMBL907374) Activity at human adenosine A1 receptor in CHO cells assessed as effect on CPA induced inhibition of forskolin-stimulated cAMP production
- ChEMBL_453754 (CHEMBL885755) Agonist activity at human NOP receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production after 2 hrs
- ChEMBL_458712 (CHEMBL942047) Agonist activity at human recombinant dopamine D2 receptor expressed in rat pituitary cells assessed as inhibition of forskolin-stimulated cAMP accumulation
- ChEMBL_479051 (CHEMBL921658) Agonist activity at GPR109A receptor expressed in CHOK1 cells assessed as inhibition of forskolin-induced intracellular cAMP production by Flashplate assay
- ChEMBL_510479 (CHEMBL1006420) Agonist activity at human recombinant CB1 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-stimulated intracellular cAMP level
- ChEMBL_510480 (CHEMBL1006421) Agonist activity at human recombinant CB2 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-stimulated intracellular cAMP level
- ChEMBL_510483 (CHEMBL1006424) Agonist activity at rat recombinant CB1 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-stimulated intracellular cAMP level
- ChEMBL_510484 (CHEMBL1006425) Agonist activity at rat recombinant CB2 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-stimulated intracellular cAMP level
- ChEMBL_515731 (CHEMBL1023733) Antagonist activity at human EP3 receptor expressed in CHO-K1 cells assessed as reversal of inhibition of forskolin-induced cAMP production
- ChEMBL_565967 (CHEMBL960863) Agonist activity at delta opioid receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production by ALPHAscreen assay
- ChEMBL_579668 (CHEMBL1064088) Agonist activity at N-terminal HA-tagged GPR109b receptor transfected in forskolin-stimulated cells assessed as cAMP accumulation by flashplate assay
- ChEMBL_579669 (CHEMBL1064089) Agonist activity at N-terminal HA-tagged GPR109a receptor transfected in forskolin-stimulated cells assessed as cAMP accumulation by flashplate assay
- ChEMBL_597154 (CHEMBL1048909) Agonist activity at human recombinant cannabinoid CB2 receptor expressed in CHOK1 cells assessed as forskolin-induced cAMP production by luciferase assay
- ChEMBL_622960 (CHEMBL1112212) Agonist activity at human NOP receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production after 2 hrs
- ChEMBL_643481 (CHEMBL1212345) Antagonist activity at human SST5 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation by FRET assay
- ChEMBL_643538 (CHEMBL1212402) Antagonist activity at CB1 receptor in forskolin-stimulated human U373-MG cells assessed as inhibition of CP-55940-induced cAMP accumulation
- ChEMBL_749353 (CHEMBL1785143) Antagonist activity at histamine H3 receptor in rat cortical hemispheres assessed as inhibition of forskolin stimulated cAMP accumulation after 6 hrs
- ChEMBL_786937 (CHEMBL1920051) Agonist activity at DRD2 Long receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation by bioluminescence assay
- ChEMBL_812809 (CHEMBL2020076) Agonist activity at human CB1 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP release after 25 mins
- ChEMBL_812810 (CHEMBL2020077) Agonist activity at human CB2 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP release after 45 mins
- ChEMBL_815226 (CHEMBL2025417) Agonist activity at human CB1 receptor expressed in CHO cells assessed as increase in forskolin-induced cAMP levels after 20 mins
- ChEMBL_815228 (CHEMBL2025419) Agonist activity at human CB2 receptor expressed in CHO cells assessed as increase in forskolin-induced cAMP levels after 20 mins
- ChEMBL_856679 (CHEMBL2162350) Agonist activity at MOP receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 30 mins
- ChEMBL_952578 (CHEMBL2354091) Agonist activity at human CB1 receptor transfected in HEK293 cells assessed as reduction in forskolin-stimulated cAMP accumulation after 30 mins
- ChEMBL_2092198 (CHEMBL4773461) Antagonist activity at CXCR1 (unknown origin) expressed in HEK293 cells assessed as suppression of IL-8-induced inhibition of forskolin-induced cAMP formation preincubated for 15 mins followed by forskolin and IL-8 stimulation and measured after 15 mins by cAMP-d2 and Eu-Anti-cAMP based fluorescence assay
- ChEBML_1597 Effective concentration determined by measuring inhibition of forskolin-stimulated c-AMP formation at 5-hydroxytryptamine 1B receptor stably transfected in CHO cell lines
- ChEMBL_1282321 (CHEMBL3100268) Agonist activity at human dopamine D2 receptor expressed in CHO cells assessed as increase of forskolin-induced cAMP production after 20 mins
- ChEMBL_1368480 (CHEMBL3300400) Activation of wild-type CFTR (unknown origin) expressed in forskolin-stimulated CHO cells assessed as increase in iodide efflux by gamma counting
- ChEMBL_139616 (CHEMBL744781) Compound was tested for its potency at Muscarinic acetylcholine receptor M2 by inhibiting forskolin induced c-AMP formation in CHO-M2 cells
- ChEMBL_139617 (CHEMBL744782) Compound was tested for its potency towards Muscarinic acetylcholine receptor M2 by inhibiting forskolin induced c-AMP formation in CHO-M2 cells
- ChEMBL_1436932 (CHEMBL3388430) Antagonist activity at human NPFF1 receptor expressed in CHO cells assessed as inhibition of forskolin-induced intracellular cAMP accumulation relative to control
- ChEMBL_1444651 (CHEMBL3376649) Agonist activity at human recombinant CB2 receptor expressing CHO cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 30 mins
- ChEMBL_1444652 (CHEMBL3376650) Agonist activity at human recombinant CB1 receptor expressing CHO cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 30 mins
- ChEMBL_1469772 (CHEMBL3413623) Agonist activity at mouse A3 adenosine receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production after 15 mins
- ChEMBL_1472331 (CHEMBL3419681) Agonist activity at rat CB1 receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 60 mins
- ChEMBL_1539094 (CHEMBL3738455) Agonist activity at delta receptor (unknown origin) expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation by bioluminescent assay
- ChEMBL_1673 (CHEMBL616664) Ability to inhibit forskolin-stimulated adenylate cyclase activity in Chinese hamster ovary (CHO) stable cell lines expressing human 5-hydroxytryptamine 1D receptor
- ChEMBL_1734784 (CHEMBL4150320) Agonist activity at human kappa opioid receptor expressed in HEK293 assessed as inhibition forskolin-induced cAMP accumulation after 15 mins by EIA
- ChEMBL_1734786 (CHEMBL4150322) Agonist activity at human mu opioid receptor expressed in HEK293 assessed as inhibition forskolin-induced cAMP accumulation after 15 mins by EIA
- ChEMBL_1766243 (CHEMBL4201490) Agonist activity at rat CB1 receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 30 mins
- ChEMBL_1857646 (CHEMBL4358375) Agonist activity at N-terminal c-Myc tagged human MasR expressed in HEK293T cells assessed as reduction in forskolin-induced cAMP accumulation
- ChEMBL_1868883 (CHEMBL4369949) Agonist activity at human A1A adenosine receptor expressed in HEK cells assessed as inhibition of forskolin-stimulated cAMP production after 60 mins
- ChEMBL_1870402 (CHEMBL4371569) Agonist activity at dopamine D2 receptor (unknown origin) assessed as inhibition of forskolin-mediated cAMP accumulation after 1 hr by FLIPR assay
- ChEMBL_1908149 (CHEMBL4410507) Agonist activity at human mu opioid receptor expressed in CHOK1 cells assessed as inhibition of forskolin-induced cAMP production by FRET assay
- ChEMBL_2051139 (CHEMBL4705838) Agonist activity at GPR88 (unknown origin) expressed in CHO cells assessed as inhibition of forskolin-induced cAMP production by TR-FRET assay
- ChEMBL_2296165 Antagonist activity at mGluR7 (unknown origin) assessed as inhibition of L-AP4 stimulated decrease in forskolin induced cAMP response by CRE-Luc assay
- ChEMBL_33481 (CHEMBL649812) Agonistic potency at alpha 2-Adrenergic receptor for inhibition of 10 uM forskolin-elicited stimulation of adenylate cyclase in human platelet membranes
- ChEMBL_341532 (CHEMBL861820) Activity against human H3 receptor as measured by CRE-mediated beta galactosidase reporter gene assay in forskolin-stimulated SK-N-MC cells
- ChEMBL_621613 (CHEMBL1115684) Antagonist activity at human SST5 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP release by TR-FRET assay
- ChEMBL_696216 (CHEMBL1639369) Antagonist activity at recombinant rat mGluR2 expressed in forskolin-stimulated CHO cells assessed as inhibition of (1S,3R)-ACPD induced cAMP production
- ChEMBL_742125 (CHEMBL1769185) Agonist activity at human dopamine D2long receptor expressed in CHO cells assessed as increase of forskolin-induced cAMP accumulation by bioluminescence assay
- ChEMBL_786940 (CHEMBL1920054) Partial agonist activity at DRD2 Long receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation by bioluminescence assay
- ChEMBL_807 (CHEMBL615786) Ability to inhibit 5-HT sensitive,forskolin-stimulated adenylyl cyclase(FSC) activity in rat hippocampal membranes mediated through 5-hydroxytryptamine 1A receptor
- ChEMBL_812480 (CHEMBL2014266) Antagonist activity at human MCH1R expressed in forskolin-stimulated CHO cells assessed as inhibition of MCH-induced cAMP accumulation after 90 mins
- ChEMBL_834579 (CHEMBL2072287) Agonist activity at human A2A adenosine receptor expressed in CHO cells assessed as stimulation of forskolin-induced cAMP accumulation after 30 mins
- ChEMBL_834865 (CHEMBL2073225) Antagonist activity at human adenosine A3 receptor expressed in CHO cells assessed as forskolin/C1-IB-MECA-induced inhibition of cAMP production
- ChEMBL_86248 (CHEMBL698014) Agonistic potency at alpha 2-Adrenergic receptor for inhibition of 10 uM forskolin-elicited stimulation of adenylate cyclase in human platelet membranes
- ChEMBL_935219 (CHEMBL2317841) Agonist activity at human CB2 receptor expressed in CHO cells assessed as forskolin-stimulated [3H]cyclic-AMP accumulation by scintillation counting analysis
- ChEMBL_952579 (CHEMBL2354092) Partial agonist activity at human CB1 receptor transfected in HEK293 cells assessed as reduction in forskolin-stimulated cAMP accumulation after 30 mins
- ChEMBL_978254 (CHEMBL2423623) Agonist activity at mouse MOR assessed as inhibition of forskolin-stimulated cAMP accumulation after 30 mins by cell based TR-FRET assay
- ChEMBL_978255 (CHEMBL2423624) Agonist activity at mouse KOR assessed as inhibition of forskolin-stimulated cAMP accumulation after 30 mins by cell based TR-FRET assay
- ChEMBL_978258 (CHEMBL2423627) Agonist activity at human KOR assessed as inhibition of forskolin-stimulated cAMP accumulation after 30 mins by cell based TR-FRET assay
- ChEMBL_978259 (CHEMBL2423628) Agonist activity at human MOR assessed as inhibition of forskolin-stimulated cAMP accumulation after 30 mins by cell based TR-FRET assay
- ChEMBL_978260 (CHEMBL2423629) Agonist activity at human DOR assessed as inhibition of forskolin-stimulated cAMP accumulation after 30 mins by cell based TR-FRET assay
- ChEMBL_989745 (CHEMBL2445983) Agonist activity at human delta opioid receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation by fluorescence assay
- ChEMBL_989746 (CHEMBL2445984) Agonist activity at human kappa opioid receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation by fluorescence assay
- ChEMBL_989756 (CHEMBL2445994) Agonist activity at human mu opioid receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation by fluorescence assay
- ChEBML_1681999 Agonist activity at human S1P1 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 30 mins by ELISA
- ChEMBL_1334808 (CHEMBL3238915) Agonist activity at human CB2 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation by TR-FRET assay
- ChEMBL_1334809 (CHEMBL3238916) Agonist activity at human CB1 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation by TR-FRET assay
- ChEMBL_1335011 (CHEMBL3239398) Agonist activity at delta opioid receptor (unknown origin) expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins
- ChEMBL_1367335 (CHEMBL3300366) Agonist activity at human HCA2 receptor expressed in Flp-In-293 cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins
- ChEMBL_1438728 (CHEMBL3388550) Agonist activity at human KOR expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by luminescence assay
- ChEMBL_1444588 (CHEMBL3375407) Agonist activity at human CB2R expressed in CHO cells assessed as reduction in forskolin-induced cAMP accumulation by beta-galactosidase based complementation immunoassay
- ChEMBL_1444589 (CHEMBL3375408) Agonist activity at human CB1R expressed in CHO cells assessed as reduction in forskolin-induced cAMP accumulation by beta-galactosidase based complementation immunoassay
- ChEMBL_1469774 (CHEMBL3413625) Agonist activity at human recombinant A3 adenosine receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production after 15 mins
- ChEMBL_1472333 (CHEMBL3419683) Inverse agonist activity at human CB2 receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 60 mins
- ChEMBL_1539093 (CHEMBL3738454) Agonist activity at mu opioid receptor (unknown origin) expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation by bioluminescent assay
- ChEMBL_1542439 (CHEMBL3743673) Agonist activity at S1P5 receptor (unknown origin) expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation by GTPgammaS binding assay
- ChEMBL_1597 (CHEMBL616624) Effective concentration determined by measuring inhibition of forskolin-stimulated c-AMP formation at 5-hydroxytryptamine 1B receptor stably transfected in CHO cell lines
- ChEMBL_1633674 (CHEMBL3876466) Activation of human S1PR4 expressed in CHO cells assessed as suppression of forskolin-induced intracellular cAMP level measured after 15 mins by ELISA
- ChEMBL_1773399 (CHEMBL4230391) Agonist activity at human KOR expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 30 mins by HTRF assay
- ChEMBL_1833167 (CHEMBL4333175) Agonist activity at NPFF2 receptor (unknown origin) expressed in HEK293A cells assessed as inhibition of forskolin-stimulated cAMP accumulation measured after 30 mins
- ChEMBL_1833195 (CHEMBL4333203) Agonist activity at NPFF1 receptor (unknown origin) expressed in HEK293A cells assessed as inhibition of forskolin-stimulated cAMP accumulation measured after 30 mins
- ChEMBL_1870394 (CHEMBL4371561) Inverse agonist activity at dopamine D2 receptor (unknown origin) assessed as inhibition of forskolin-mediated cAMP accumulation after 20 mins by radiometric assay
- ChEMBL_2019530 (CHEMBL4673108) Inverse agonist activity at human CB1 receptor expressed in HEK293 cells assessed as forskolin-stimulated cAMP accumulation after 30 mins by HTRF assay
- ChEMBL_2019531 (CHEMBL4673109) Inverse agonist activity at human CB2 receptor expressed in HEK293 cells assessed as forskolin-stimulated cAMP accumulation after 30 mins by HTRF assay
- ChEMBL_2090979 (CHEMBL4772242) Partial agonist activity at NPFFR1 (unknown origin) expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 30 mins
- ChEMBL_2152464 (CHEMBL5037011) Potentiation of CFTR F508del mutant (unknown origin) expressed in FRT cells assessed as chloride transport by measuring membrane potential in presence of forskolin
- ChEMBL_2152466 (CHEMBL5037013) Potentiation of wild-type CFTR (unknown origin) expressed in FRT cells assessed as chloride transport by measuring membrane potential in presence of forskolin
- ChEMBL_2152468 (CHEMBL5037015) Potentiation of CFTR G551D mutant (unknown origin) expressed in FRT cells assessed as chloride transport by measuring membrane potential in presence of forskolin
- ChEMBL_2245454 (CHEMBL5159664) Antagonist activity at human GPR84 expressed in HEK293 cells assessed as reversal of 6-OAU induced inhibition of forskolin induced intracellular cAMP production
- ChEMBL_353098 (CHEMBL866942) Activity against human adenosine A1 receptor in CHO cells assessed by the antagonizing effect of 100 nM CPA on forskolin-induced cAMP production
- ChEMBL_376266 (CHEMBL864777) Agonist activity at human 5HT1A receptor expressed in CHO cells by inhibition of 8-OH-DPAT-induced decrease in forskolin-stimulated cAMP production
- ChEMBL_450834 (CHEMBL899920) Agonist activity at human GPR109a receptor transfected in CHOK1 cells assessed as reversal of forskolin induced cAMP elevating effect by whole cell assay
- ChEMBL_477429 (CHEMBL929415) Antagonist activity at human muscarinic M4 receptor expressed in CHO cells assessed as blockade of carbachol-induced inhibition of forskolin-stimulated cAMP accumulation
- ChEMBL_518789 (CHEMBL960425) Agonist activity at human adenosine A3 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP release by TR-FRET assay
- ChEMBL_562709 (CHEMBL1012744) Antagonist activity at human adenosine A3 receptor expressed in CHO cells assessed as blockade of NECA-mediated inhibition of forskolin-stimulated cAMP accumulation
- ChEMBL_583151 (CHEMBL1062530) Agonist activity at rat cloned kappa opioid receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP production by scintillation counting
- ChEMBL_615554 (CHEMBL1108616) Inverse agonist activity at human cannabinoid CB1 receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 60 mins
- ChEMBL_630796 (CHEMBL1105738) Inverse agonist activity at human recombinant cannabinoid CB1 receptor expressed in CHO cellssassessed as inhibition of forskolin-stimulated increase in intracellular cAMP level
- ChEMBL_651412 (CHEMBL1227385) Inhibition of CFTR in nonpermeabilized human T84 cells assessed as inhibition of forskolin and IBMX-induced chloride current by short-circuit current analysis
- ChEMBL_743881 (CHEMBL1767824) Agonist activity at human SST2 expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP release after 40 mins by luminescence assay
- ChEMBL_834578 (CHEMBL2072286) Partial agonist activity at human A1 adenosine receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins
- ChEMBL_851389 (CHEMBL2156219) Agonist activity at human MOR expressed in HEK293 cell membrane assessed as inhibition of forskolin-induced cAMP accumulation by by liquid scintillation counting
- ChEMBL_851391 (CHEMBL2156324) Agonist activity at human DOR expressed in HEK293 cell membrane assessed as inhibition of forskolin-induced cAMP accumulation by by liquid scintillation counting
- ChEMBL_874994 (CHEMBL2183870) Agonist activity at human MOR expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation after 15 mins by cAMP EIA
- ChEMBL_938962 (CHEMBL2328544) Agonist activity at human NPY2R expressed inj CHOK1 cells assessed as inhibition of forskolin-stimulated cAMP production after 30 mins by luminescence assay
- ChEMBL_106038 (CHEMBL717361) Ability to block 1S,3R-ACPD-induced inhibition of forskolin stimulated cyclic-AMP in non-neuronal cell line expressing human Metabotropic glutamate receptor 2
- ChEMBL_106379 (CHEMBL718352) Ability to block 1S,3R-ACPD-induced inhibition of forskolin stimulated cyclic-AMP in non-neuronal cell line expressing human Metabotropic glutamate receptor 3
- ChEMBL_139614 (CHEMBL880146) Compound was tested for its potency at M-2 receptor by inhibiting forskolin induced c-AMP formation in CHO-M2 cells human M2 receptor
- ChEMBL_1436257 (CHEMBL3387757) Agonist activity at human NPFF1 receptor expressed in CHO cells assessed as inhibition of forskolin-induced intracellular cAMP accumulation by liquid scintillation counting analysis
- ChEMBL_1436258 (CHEMBL3387758) Agonist activity at human NPFF2 receptor expressed in CHO cells assessed as inhibition of forskolin-induced intracellular cAMP accumulation by liquid scintillation counting analysis
- ChEMBL_1474637 (CHEMBL3423987) Agonist activity at human NPFF1R expressed in CHO cells assessed as effect on forskolin-induced cAMP accumulation after 15 mins by luminescence based assay
- ChEMBL_1476599 (CHEMBL3428418) Modulation of human histamine H3 receptor expressed in HEK293 cells assessed as change in forskolin-stimulated cAMP level after 1 hr by HTRF assay
- ChEMBL_1504576 (CHEMBL3591317) Agonist activity at delta opioid receptor (unknown origin) expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation by FRET assay
- ChEMBL_1505662 (CHEMBL3596031) Agonist activity at human 5-HT1A receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 15 mins by ELISA
- ChEMBL_1522303 (CHEMBL3630726) Antagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassay
- ChEMBL_1633736 (CHEMBL3876528) Positive allosteric modulation of human mGlu2 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production by high throughput screening assay
- ChEMBL_1673191 (CHEMBL4023220) Agonist activity at human mGlu2 receptor expressed in HEK cells assessed as inhibition of forskolin-stimulated cAMP formation after 20 mins by HTRF assay
- ChEMBL_1673195 (CHEMBL4023224) Agonist activity at human mGlu3 receptor expressed in HEK cells assessed as inhibition of forskolin-stimulated cAMP formation after 20 mins by HTRF assay
- ChEMBL_1681999 (CHEMBL4032276) Agonist activity at human S1P1 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 30 mins by ELISA
- ChEMBL_1682000 (CHEMBL4032277) Agonist activity at human S1P5 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 30 mins by ELISA
- ChEMBL_1705795 (CHEMBL4057028) Agonist activity at human kappa opioid receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation by TR-FRET assay
- ChEMBL_1705796 (CHEMBL4057029) Agonist activity at human mu opioid receptor expressed in CHO-dhfr(-) cells assessed as inhibition of forskolin-induced cAMP accumulation by TR-FRET assay
- ChEMBL_1739334 (CHEMBL4155084) Agonist activity at CXCR4 (unknown origin) expressed in HEK293T cells assessed as inhibition of forskolin-mediated cAMP accumulation after 20 mins by BRET assay
- ChEMBL_1740206 (CHEMBL4155956) Agonist activity at recombinant full length human GPR18 expressed in CHO cells assessed as forskolin-stimulated cAMP accumulation after 10 mins by enzyme immunoassay
- ChEMBL_1743005 (CHEMBL4158755) Agonist activity at human MOR expressed in CHOK1 cells assessed as reduction in forskolin-induced intracellular cAMP increase after 45 mins by HTRF analysis
- ChEMBL_1833162 (CHEMBL4333170) Agonist activity at mu-opioid receptor (unknown origin) expressed in HEK293A cells assessed as inhibition of forskolin-induced cAMP accumulation measured after 30 mins
- ChEMBL_1833163 (CHEMBL4333171) Agonist activity at delta-opioid receptor (unknown origin) expressed in HEK293A cells assessed as inhibition of forskolin-induced cAMP accumulation measured after 30 mins
- ChEMBL_1870384 (CHEMBL4371551) Agonist activity at human NOPR expressed in human BHK cells assessed as inhibition of forskolin-mediated cAMP accumulation after 10 mins by radioimmuno assay
- ChEMBL_1870390 (CHEMBL4371557) Agonist activity at YFP-fused human NOPR expressed in HEK293 cells assessed as inhibition of forskolin-mediated cAMP accumulation measured immediately by luminescence assay
- ChEMBL_1905180 (CHEMBL4407538) Antagonist activity at human A1AR expressed in CHO cells assessed as suppression of CCPA-induced inhibition of forskolin-stimulated cAMP production by alphascreen assay
- ChEMBL_198197 (CHEMBL799125) Compound was tested for its ability to inhibit forskolin-stimulated activity of adenylate cyclase coupled to human 5-HT 1A receptor in HeLa cells
- ChEMBL_2026352 (CHEMBL4680510) Agonist activity at human MOR expressed in CHOK1 cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 30 mins by luminescence assay
- ChEMBL_2026354 (CHEMBL4680512) Agonist activity at human KOR expressed in CHOK1 cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 30 mins by luminescence assay
- ChEMBL_2026356 (CHEMBL4680514) Agonist activity at human DOR expressed in CHOK1 cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 30 mins by luminescence assay
- ChEMBL_2057177 (CHEMBL4712178) Agonist activity at human GPR84 expressed in HEK293 cells assessed as reduction in forskolin-stimulated cAMP production incubated for 60 mins by HTRF assay
- ChEMBL_2061713 (CHEMBL4716966) Agonist activity at human RXFP4 expressed in CHO-K1 cells assessed as reduction in forskolin-induced cAMP production by pCRE beta-galactosidase reporter assay
- ChEMBL_2064771 (CHEMBL4720024) Inverse agonist activity at human CB1 receptor expressed in HEK293 cells assessed as forskolin-stimulated cAMP accumulation measured after 30 mins by HTRF assay
- ChEMBL_2064772 (CHEMBL4720025) Inverse agonist activity at human CB2 receptor expressed in HEK293 cells assessed as forskolin-stimulated cAMP accumulation measured after 30 mins by HTRF assay
- ChEMBL_2090948 (CHEMBL4772211) Agonist activity at kappa opioid receptor (unknown origin) expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 30 mins
- ChEMBL_2090949 (CHEMBL4772212) Agonist activity at mu-opioid receptor (unknown origin) expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 30 mins
- ChEMBL_2090978 (CHEMBL4772241) Agonist activity at delta-opioid receptor (unknown origin) expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 30 mins
- ChEMBL_2152472 (CHEMBL5037019) Potentiation of wild type CFTR (unknown origin) expressed in HBE cells assessed as change in short circuit current in presence of forskolin and amiloride
- ChEMBL_2214397 (CHEMBL5127529) Agonist activity at MOP in human SH-SY5Y cells assessed as forskolin stimulated cAMP accumulation incubated for 30 mins by Biotrak-EIA kit method
- ChEMBL_2290775 Agonist activity at delta opioid receptor (unknown origin) expressed in human HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation by Glo Sensor assay
- ChEMBL_407554 (CHEMBL907109) Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-induced cAMP-mediated CRE-beta galactosidase activity
- ChEMBL_407556 (CHEMBL907116) Agonist activity at human histamine H3 receptor in SK-N-MC cells assessed as inhibition of forskolin-induced cAMP-mediated CRE-beta galactosidase activity
- ChEMBL_596835 (CHEMBL1042646) Antagonist activity at human EP3 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP level after 10 mins by EIA
- ChEMBL_699225 (CHEMBL1646947) Antagonist activity at human CRTH2 expressed in HEK293 cells assessed as inhibition of forskolin-induced increase intracellular [125I]cAMP level by scintillation proximity assay
- ChEMBL_735236 (CHEMBL1694498) Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay
- ChEMBL_735237 (CHEMBL1694499) Agonist activity at human histamine H3 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay
- ChEMBL_769085 (CHEMBL1832642) Inhibition of human wild-type CFTR expressed in FRT cells assessed as inhibition of forskolin-induced short-circuit current by voltage clamp electrophysiology assay
- ChEMBL_786287 (CHEMBL1920392) Agonist activity at human S1P1 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production after 1 hr by HTRF assay
- ChEMBL_786289 (CHEMBL1920394) Agonist activity at mouse S1P1 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production after 1 hr by HTRF assay
- ChEMBL_786290 (CHEMBL1920395) Agonist activity at rat S1P1 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production after 1 hr by HTRF assay
- ChEMBL_812056 (CHEMBL2013482) Agonist activity at human recombinant CB2 receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP production after 20 mins by ELISA
- ChEMBL_812058 (CHEMBL2013484) Agonist activity at rat recombinant CB2 receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP production after 20 mins by ELISA
- ChEMBL_812161 (CHEMBL2013665) Agonist activity at human recombinant CB1 receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP production after 20 mins by ELISA
- ChEMBL_812163 (CHEMBL2013667) Agonist activity at rat recombinant CB1 receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP production after 20 mins by ELISA
- ChEMBL_856460 (CHEMBL2161363) Agonist activity at mu-opioid receptor in human SH-SY5Y cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 30 mins by immunoassay
- ChEMBL_874992 (CHEMBL2183868) Partial agonist activity at human MOR expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation after 15 mins by cAMP EIA
- ChEMBL_959201 (CHEMBL2383556) Inverse agonist activity at human CB2 receptor expressed in CHO cell membrane assessed as increase in forskolin-stimulated cAMP production by scintillation counting analysis
- ChEMBL_993379 (CHEMBL2444574) Antagonist activity at human CRTh2 receptor expressed in CHO cells assessed as inhibition of prostaglandin D2 and forskolin-induced cAMP accumulation after 45 mins
- ChEBML_1643866 Agonist activity at human MOR expressed in HEK293 cells assessed as inhibition of forskolin-induced adenylyl cyclase-mediated cAMP accumulation after 30 mins by HTRF assay
- ChEBML_1643867 Agonist activity at human DOR expressed in HEK293 cells assessed as inhibition of forskolin-induced adenylyl cyclase-mediated cAMP accumulation after 30 mins by HTRF assay
- ChEBML_1643868 Agonist activity at human KOR expressed in HEK293 cells assessed as inhibition of forskolin-induced adenylyl cyclase-mediated cAMP accumulation after 30 mins by HTRF assay
- ChEBML_218840 Tested for agonist activity in non-neuronal cells (RGT) expressing human mGlu7a receptor by measuring L-AP4 (1000 uM of forskolin (15 uM)-stimulated cAMP formation
- ChEMBL_1290721 (CHEMBL3117733) Agonist activity at human dopamine D2L receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by LANCE assay
- ChEMBL_1460276 (CHEMBL3370192) Agonist activity at human mu opioid receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP production after 30 mins by HTRF assay
- ChEMBL_1460277 (CHEMBL3370193) Agonist activity at human delta opioid receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP production after 30 mins by HTRF assay
- ChEMBL_1460278 (CHEMBL3370194) Agonist activity at human kappa opioid receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP production after 30 mins by HTRF assay
- ChEMBL_1461284 (CHEMBL3396058) Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity
- ChEMBL_1471051 (CHEMBL3420496) Antagonist activity at human recombinant adenosine A3 receptor transfected in CHO cells assessed as reversal of NECA-induced inhibition of forskolin-induced adenylyl cyclase activity
- ChEMBL_1518588 (CHEMBL3619271) Agonist activity at human recombinant mGlu2 receptor expressed in AV12 cells assessed as inhibition of forskolin-stimulated cAMP production after 1 hr by fluorescence assay
- ChEMBL_1518595 (CHEMBL3619410) Agonist activity at human recombinant mGlu3 receptor expressed in AV12 cells assessed as inhibition of forskolin-stimulated cAMP production after 1 hr by fluorescence assay
- ChEMBL_1518609 (CHEMBL3619424) Agonist activity at human recombinant mGlu6 receptor expressed in AV12 cells assessed as inhibition of forskolin-stimulated cAMP production after 1 hr by fluorescence assay
- ChEMBL_1518613 (CHEMBL3619428) Agonist activity at human recombinant mGlu8 receptor expressed in AV12 cells assessed as inhibition of forskolin-stimulated cAMP production after 1 hr by fluorescence assay
- ChEMBL_1651010 (CHEMBL4000265) Agonist activity at CB1 receptor in rat brain membranes assessed as reduction in forskolin-stimulated cAMP level measured from 3 data points after 30 mins
- ChEMBL_1651011 (CHEMBL4000266) Agonist activity at CB1 receptor in rat brain membranes assessed as reduction in forskolin-stimulated cAMP level measured from 8 data points after 30 mins
- ChEMBL_1705579 (CHEMBL4056812) Agonist activity at recombinant human HCA2 receptor expressed in Flp-IN HEK cells assessed as reduction in forskolin-stimulated cAMP accumulation measured after 30 mins
- ChEMBL_1803969 (CHEMBL4276261) Inverse agonist activity at human CB1 receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by HTRF assay
- ChEMBL_1803971 (CHEMBL4276263) Inverse agonist activity at human CB2 receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by HTRF assay
- ChEMBL_1824655 (CHEMBL4324419) Agonist activity at human APJ-R expressed in HEK293 cells assessed as inhibition of forskolin- stimulated cAMP accumulation incubated for 30 mins by HTRF assay
- ChEMBL_1824656 (CHEMBL4324420) Agonist activity at rat APJ-R expressed in HEK293 cells assessed as inhibition of forskolin- stimulated cAMP accumulation incubated for 30 mins by HTRF assay
- ChEMBL_1843344 (CHEMBL4343771) Agonist activity at recombinant human GPR84 expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation by fluo-4AM dye based FLIPR assay
- ChEMBL_1870443 (CHEMBL4371610) Inverse agonist activity at mouse CB2 receptor expressed in HEK293 cells assessed as inhibition of forskolin-mediated cAMP accumulation after 5 mins by fluorescence assay
- ChEMBL_1882345 (CHEMBL4383844) Inhibition of DAPK1 in HEK293 tau-BiFC cells assessed as reduction in forskolin-induced tau aggregation after 48 hrs by Hoechst staining based HCS analysis
- ChEMBL_1923192 (CHEMBL4426148) Agonist activity at human CB2 receptor expressed in HEK293 cell membranes assessed as inhibition of forskolin-stimulated cAMP accumulation after 60 mins by HTRF assay
- ChEMBL_201049 (CHEMBL803826) Compound was tested for its ability inhibit forskolin-stimulated activity of adenylate cyclase coupled to human 5-HT 1D-beta receptor in CHO-K1 cells
- ChEMBL_201050 (CHEMBL803827) Compound was tested for its ability inhibit forskolin-stimulated activity of adenylate cyclase coupled to human 5-HT 1D-beta receptor in CHO-K1 cells
- ChEMBL_2057170 (CHEMBL4712171) Agonist activity at human GPR84 expressed in CHO-K1 cells assessed as reduction in forskolin-stimulated cAMP production incubated for 30 mins by luminescence assay
- ChEMBL_2090980 (CHEMBL4772243) Agonist activity at human NPFFR2 expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 30 mins by LANCE cAMP assay
- ChEMBL_2125824 (CHEMBL4835169) Agonist activity at human MT1 expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 30 mins by AlphaScreen assay
- ChEMBL_2125825 (CHEMBL4835170) Agonist activity at human MT2 expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 30 mins by AlphaScreen assay
- ChEMBL_2152470 (CHEMBL5037017) Potentiation of CFTR F508 deletion mutant (unknown origin) expressed in HBE cells assessed as change in short circuit current in presence of forskolin and amiloride
- ChEMBL_2186719 (CHEMBL5098801) Agonist activity at human APJ expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production incubated for 60 mins by fluorescence based analysis
- ChEMBL_2193272 (CHEMBL5105632) Agonist activity at human APJ receptor expressed in HEK293 cells assessed as compound stimulated inhibition of forskolin stimulated cAMP production and measured by FLIPR assay
- ChEMBL_2271483 Potentiation of CFTR F508del mutant (unknown origin) expressed in rat FRT cells assessed as increase in iodide influx in presence of forskolin incubated for 24 hrs
- ChEMBL_2290773 Agonist activity at HA-tagged mouse mu opioid receptor expressed in human HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation by Glo Sensor assay
- ChEMBL_2290777 Agonist activity at FLAG-tagged mouse kappa opioid receptor expressed in human HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation by Glo Sensor assay
- ChEMBL_496464 (CHEMBL1004595) Antagonist activity at human histamine H3 receptor expressed in HEK293 cells assessed as reversal of N-alpha-methylhistamine-induced inhibition of forskolin-stimulated cAMP formation
- ChEMBL_592556 (CHEMBL1046763) Agonist activity at rat delta opioid receptor expressed in rat C6 cells assessed as inhibition of forskolin-stimulated cAMP production after 30 mins by radioimmunoassay
- ChEMBL_640777 (CHEMBL1175769) Antagonist activity at rat histamine H3 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP accumulation after 6 hrs
- ChEMBL_651195 (CHEMBL1228135) Inhibition of CFTR in nonpermeabilized human bronchial epithelial cells assessed as inhibition of forskolin and IBMX-induced chloride current at by short-circuit current analysis
- ChEMBL_735240 (CHEMBL1694502) Inverse agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay
- ChEMBL_735241 (CHEMBL1694503) Inverse agonist activity at human histamine H3 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay
- ChEMBL_744295 (CHEMBL1772152) Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs
- ChEMBL_755988 (CHEMBL1803964) Agonist activity at human MT1 receptor expressed in CHO cells assessed as decrease in forskolin-stimulated cAMP release after 30 mins by multilabel plate reader
- ChEMBL_755989 (CHEMBL1803965) Agonist activity at human MT2 receptor expressed in CHO cells assessed as decrease in forskolin-stimulated cAMP release after 30 mins by multilabel plate reader
- ChEMBL_772741 (CHEMBL1837353) Agonist activity at human recombinant CB2 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP formation after 45 mins by fluorescence assay
- ChEMBL_826808 (CHEMBL2051429) Agonist activity at human mu opioid receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 1 hr by HTRF assay
- ChEMBL_834525 (CHEMBL2073574) Corrector activity at human CFTR deltaF508 mutant expressed in FRT cells stimulated forskolin and potentiator genistein with assessed as iodide influx incubated for 24 hrs
- ChEMBL_859256 (CHEMBL2166341) Agonist activity at mu opioid receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by liquid scintillation counter
- ChEMBL_959780 (CHEMBL2383352) Agonist activity at CB1 receptor in mouse Neuro2a cells assessed as inhibition of forskolin-stimulated adenylyl cyclase activity after 15 mins by liquid scintillation spectrophotometry
- ChEMBL_961621 (CHEMBL2388938) Antagonist activity at human CRTH2 receptor expressed in HEK293 cells assessed as inhibition of PGD2/forskolin-induced intracellular cAMP production after 20 mins by ELISA
- ChEMBL_1369055 (CHEMBL3300168) Agonist activity at human D2L receptor expressed in FlpIn CHO cells assessed as inhibition of forskolin-stimulated cAMP production treated for 30 mins by AlphaScreen assay
- ChEMBL_1545458 (CHEMBL3748920) Corrector activity at human CFTR F508 deletion mutant expressed in FRT cells incubated for 25 mins with forskolin by YFP-based fluorescence analysis relative to control
- ChEMBL_1601 (CHEMBL616627) The intrinsic activity was evaluated for its ability to inhibit forskolin-stimulated c-AMP formation mediated by cloned 5-hydroxytryptamine 1B receptor in CHO cell line
- ChEMBL_1643866 (CHEMBL3992795) Agonist activity at human MOR expressed in HEK293 cells assessed as inhibition of forskolin-induced adenylyl cyclase-mediated cAMP accumulation after 30 mins by HTRF assay
- ChEMBL_1643867 (CHEMBL3992796) Agonist activity at human DOR expressed in HEK293 cells assessed as inhibition of forskolin-induced adenylyl cyclase-mediated cAMP accumulation after 30 mins by HTRF assay
- ChEMBL_1643868 (CHEMBL3992797) Agonist activity at human KOR expressed in HEK293 cells assessed as inhibition of forskolin-induced adenylyl cyclase-mediated cAMP accumulation after 30 mins by HTRF assay
- ChEMBL_1676750 (CHEMBL4026893) Agonist activity at recombinant human muscarinic M2 receptor expressed in HEK293 cells assessed as inhibition of forskolin-mediated cAMP accumulation after 10 mins by FRET assay
- ChEMBL_1816961 (CHEMBL4316535) Corrector activity at forskolin-stimulated CFTR F508del mutant in human NHBE cells after 18 to 24 hrs in presence of GLPG1873 by voltage-clamp based assay
- ChEMBL_2086891 (CHEMBL4768154) Potentiator activity at CFTR F508del mutant (unknown origin) expressed in CFBE41o cells assessed as increase in forskolin induced transepithelial short circuit current by Ussing chamber assay
- ChEMBL_2094518 (CHEMBL4775781) Agonist activity at human CB2 receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP production incubated for 15 mins by cAMP HTRF assay
- ChEMBL_2114591 (CHEMBL4823532) Agonist activity at human APJ receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation measured after 40 mins by TR-FRET assay
- ChEMBL_2125495 (CHEMBL4834840) Agonist activity at human MT1 receptor expressed in CHO cells assessed as increase in forskolin induced cAMP production incubated at 37 degreeC by HitHunter-cAMP assay
- ChEMBL_2132047 (CHEMBL4841562) Agonist activity at human SSTR2 expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation measured after 30 mins by GloSensor cAMP Assay
- ChEMBL_2145918 (CHEMBL5030198) Agonist activity at mu-opioid receptor (unknown origin) expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 15 mins by ELISA
- ChEMBL_2147437 (CHEMBL5031783) Agonist activity at human MOR expressed in HEK293T cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 30 mins by Glo-sensor cAMP assay
- ChEMBL_2149055 (CHEMBL5033453) Agonist activity at human MOR expressed in CHO-K1 cells assessed as reduction in forskolin-induced cAMP production incubated for 45 mins by by HTRF assay
- ChEMBL_2151143 (CHEMBL5035605) Agonist activity at human MOR expressed in CHO-K1 cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 60 mins by cAMP HTRF assay
- ChEMBL_2158959 (CHEMBL5043709) Antagonist activity at N-terminal tagged human CB1 receptor transfected with HEK293 cells assessed as forskolin-stimulated cAMP level in presence of CP55940 by BRET assay
- ChEMBL_2197776 (CHEMBL5110292) Agonist activity at human MOR expressed in CHO-K1 cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 45 mins by cAMP HTRF assay
- ChEMBL_2271481 Corrector activity at CFTR F508del mutant (unknown origin) expressed in rat FRT cells assessed as increase in iodide influx in presence of forskolin incubated for 24 hrs
- ChEMBL_472696 (CHEMBL923880) Activity at human adenosine A1 receptor expressed in CHO cells assessed as inhibition of CPA-induced decrease in forskolin-stimulated cAMP production by TR-FRET assay
- ChEMBL_595741 (CHEMBL1045264) Agonist activity at human P2Y14 receptor expressed in HEK293 cells coexpressing phospholipase C-activating Gi protein cells assessed as inhibition of forskolin induced [3H]cAMP production
- ChEMBL_632234 (CHEMBL1110096) Antagonist activity at human 5HT1A receptor expressed in CHO cells assessed as blockade of 8-OH-DPAT-induced inhibition of forskolin-stimulated increase in cAMP level
- ChEMBL_644979 (CHEMBL1211365) Positive allosteric modulation of human mGluR4 expressed in CHO-K1 cells assessed as decrease in forskolin-induced intracellular cAMP accumulation after 1 hr by fluorescence assay
- ChEMBL_748140 (CHEMBL1781819) Antagonist activity at human CRTH2 receptor expressed in HEK293-EBNA cells assessed as inhibition of forskolin-stimulated intracellular cAMP production by [125I]-cAMP scintillation proximity assay
- ChEMBL_785118 (CHEMBL1920856) Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay
- ChEMBL_798257 (CHEMBL1943644) Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay
- ChEMBL_818422 (CHEMBL2034649) Agonist activity at RXFP4 expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation after 6 hrs by beta galactosidase reporter gene assay
- ChEMBL_818423 (CHEMBL2034650) Agonist activity at RXFP3 expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation after 6 hrs by beta galactosidase reporter gene assay
- ChEMBL_859787 (CHEMBL2169615) Agonist activity at human A1AR expressed in HEK293T/17 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 10 mins by luciferase reporter assay
- ChEMBL_946010 (CHEMBL2341541) Agonist activity at human CB2 receptor expressed in CHO cell membranes assessed as increase in forskolin-stimulated cAMP production after 45 mins by TR-FRET assay
- ChEMBL_959779 (CHEMBL2383351) Agonist activity at human CB2 receptor transfected in CHO cells assessed as inhibition of forskolin-stimulated adenylyl cyclase activity after 15 mins by liquid scintillation spectrophotometry
- ChEMBL_960597 (CHEMBL2389886) Inhibition of wild type human CFTR chloride conductance expressed in forskolin-stimulated rat FRT cells by short-circuit current analysis in presence of transepithelial chloride gradient
- ChEBML_104491 Tested for agonist activity in non-neuronal cells (RGT) expressing human Metabotropic glutamate receptor 8 by measuring L-AP4 (0.3 uM of forskolin) (15 uM)-stimulated cAMP formation
- ChEBML_143829 Functional Neuropeptide Y receptor type 1 antagonism was evaluated by its ability to reverse NPY induced inhibition of forskolin-stimulated cyclic AMP production in SK-N-MC cells
- ChEBML_1603 The intrinsic activity was evaluated for its ability to inhibit forskolin-stimulated c-AMP formation mediated by cloned 5-hydroxytryptamine 1B receptor in CHO cell line; Full Agonist
- ChEMBL_1336427 (CHEMBL3242431) Agonist activity at rat apelin receptor expressed in CHO cell membranes coexpressing EGFP assessed as inhibition of forskolin-induced cAMP production after 30 mins by fluorescence analysis
- ChEMBL_1465054 (CHEMBL3405070) Agonist activity at GPR88 (unknown origin) transfected in forskolin-stimulated HEK cells assessed as inhibition of cAMP production after 30 mins by HTRF method relative to control
- ChEMBL_1471754 (CHEMBL3421346) Agonist activity at human APJ receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced intracellular cAMP production incubated for 30 mins by TR-FRET assay
- ChEMBL_1502794 (CHEMBL3591197) Activity at hemagglutinin-tagged human CB1 receptor expressed in HEK293 cells assessed as effect on forskolin-induced cAMP accumulation in presence of CP55,940 by cAMP BRET assay
- ChEMBL_1502804 (CHEMBL3591207) Activity at hemagglutinin-tagged human CB2 receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation in absence of CP55,940 by cAMP BRET assay
- ChEMBL_1602 (CHEMBL616628) The intrinsic activity was evaluated for its ability to inhibit forskolin-stimulated c-AMP formation mediated by cloned 5-hydroxytryptamine 1B receptor in CHO cell line; Antagonist
- ChEMBL_1649652 (CHEMBL3998786) Activation of human CFTR expressed in forskolin-stimulated basolateral membrane permeabilized FRT cells co-expressing YFP assessed as increase in short-circuit current by voltage clamp method
- ChEMBL_1651029 (CHEMBL4000284) Agonist activity at human dopamine D2L receptor expressed in F1pIn CHO cells assessed as increase in forskolin-mediated cAMP accumulation incubated for 30 mins by AlphaScreen assay
- ChEMBL_1673193 (CHEMBL4023222) Antagonist activity at human mGlu2 receptor expressed in HEK cells assessed as reversal of DCG-4 inhibited forskolin-stimulated cAMP formation after 20 mins by HTRF assay
- ChEMBL_1673197 (CHEMBL4023226) Antagonist activity at human mGlu3 receptor expressed in HEK cells assessed as reversal of DCG-4 inhibited forskolin-stimulated cAMP formation after 20 mins by HTRF assay
- ChEMBL_1677782 (CHEMBL4027925) Antagonist activity at recombinant human adenosine A3 receptor expressed in CHO cell membranes assessed as inhibition of forskolin-stimulated adenylyl cyclase in presence of [alpha-31P]ATP
- ChEMBL_1742408 (CHEMBL4158158) Agonist activity at human FFA2R expressed in Flp-InT-REx 293 cells assessed as reduction of forskolin-induced cAMP production after 30 mins by TR-FRET assay
- ChEMBL_1742444 (CHEMBL4158194) Agonist activity at mouse FFA2R expressed in Flp-InT-REx 293 cells assessed as reduction of forskolin-induced cAMP production after 30 mins by TR-FRET assay
- ChEMBL_1798123 (CHEMBL4270240) Antagonist activity at human SSTR5 expressed in CHO-K1 cell membranes assessed as reduction in SST-28-induced inhibition of forskolin-stimulated cAMP accumulation by LANCE assay
- ChEMBL_1798124 (CHEMBL4270241) Antagonist activity at mouse SSTR5 expressed in CHO-K1 cell membranes assessed as reduction in SST-28-induced inhibition of forskolin-stimulated cAMP accumulation by LANCE assay
- ChEMBL_1808995 (CHEMBL4308354) Agonist activity at human 5 HT1A receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 30 mins by TR-FRET assay
- ChEMBL_1833165 (CHEMBL4333173) Antagonist activity at NPFF1 receptor (unknown origin) expressed in HEK293A cells assessed as reversal of NPPF induced inhibition of forskolin-stimulated cAMP accumulation measured after 30 mins
- ChEMBL_1833166 (CHEMBL4333174) Antagonist activity at NPFF2 receptor (unknown origin) expressed in HEK293A cells assessed as reversal of NPPF induced inhibition of forskolin-stimulated cAMP accumulation measured after 30 mins
- ChEMBL_1878397 (CHEMBL4379791) Allosteric agonist activity at human CB1R expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation after 90 mins by hit-hunter cAMP assay
- ChEMBL_1909047 (CHEMBL4411493) Agonist activity at recombinant human Cb2 receptor expressed in CHOK1 cells assessed as inhibition of forskolin-stimulated cAMP levels measured up to 30 mins by luminescence assay
- ChEMBL_1929025 (CHEMBL4432201) Agonist activity at human RXFP3 receptor expressed in CHOK1 cells cotransfected with beta-galactosidase assessed as inhibition of forskolin-stimulated cAMP by beta-galactosidase reporter gene assay
- ChEMBL_1929027 (CHEMBL4432203) Agonist activity at human RXFP4 receptor expressed in CHOK1 cells cotransfected with beta-galactosidase assessed as inhibition of forskolin-stimulated cAMP by beta-galactosidase reporter gene assay
- ChEMBL_2046249 (CHEMBL4700948) Activation of human NPFF2R overexpressing CHO cells assessed as inhibition of forskolin-stimulated cAMP degradation incubated for 30 mins in presence of IBMX by fluorescence based assay
- ChEMBL_2112153 (CHEMBL4821003) Agonist activity at human APJ receptor expressed in human HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 1 hr by TR-FRET assay
- ChEMBL_2112154 (CHEMBL4821004) Agonist activity at rat APJ receptor expressed in human HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 1 hr by TR-FRET assay
- ChEMBL_2130818 (CHEMBL4840247) Agonist activity at human APJ receptor expressed in HEK293T cells assessed as compound stimulated inhibition of forskolin stimulated cAMP production incubated for 30 mins by HTRF assay
- ChEMBL_2130819 (CHEMBL4840248) Agonist activity at rat APJ receptor expressed in HEK293T cells assessed as compound stimulated inhibition of forskolin stimulated cAMP production incubated for 30 mins by HTRF assay
- ChEMBL_2149552 (CHEMBL5033950) Agonist activity at human RXFP3 expressed in human CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation measured after 1.5 hrs by TR-FRET assay
- ChEMBL_2149557 (CHEMBL5033955) Agonist activity at human RXFP4 expressed in human CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation measured after 1.5 hrs by TR-FRET assay
- ChEMBL_2160896 (CHEMBL5045646) Antagonist activity at human P2Y14 expressed in CHO cells assessed as inhibition of forskolin-induced increase of cAMP accumulation incubated for 15 mins in presence of IBMX
- ChEMBL_2197778 (CHEMBL5110294) Partial agonist activity at human MOR expressed in CHO-K1 cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 45 mins by cAMP HTRF assay
- ChEMBL_2199482 (CHEMBL5111998) Antagonist activity at wild type A1R (unknown origin) expressed in CHO-K1 cells assessed as inhibition of NECA reduced forskolin stimulated cAMP accumulation incubated for 30 mins
- ChEMBL_2201662 (CHEMBL5114370) Potentiation of CFTR in FRT cells co-expressing human wild-type CFTR and YFP iodide-sensing protein in presence of forskolin measured by High-Throughput Screening method
- ChEMBL_2214383 (CHEMBL5127515) Agonist activity at MOP (unknown origin) stably expressed in HEK293 cells assessed as forskolin stimulated cAMP accumulation incubated for 30 mins by radioactivity based scintillation counter analysis
- ChEMBL_2239232 (CHEMBL5153128) Antagonist activity at P2Y14R (unknown origin) expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated in presence of IBMX by cAMP-glo assay
- ChEMBL_2243597 (CHEMBL5157807) Agonist activity at rat CB1 receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation measured for 60 mins by Lance Ultra cAMP assay
- ChEMBL_2283014 Agonist activity at human HCA2 expressed in human Flp-In-293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 30 mins by Lance cAMP assay
- ChEMBL_2292479 Agonist activity at human kappa opioid receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-stimulated cAMP production incubated for 30 mins by LANCE cAMP assay
- ChEMBL_797951 (CHEMBL1942828) Antagonist activity at mouse NPBWR1 expressed in CHO-K1 cells assessed as inhibition of hNPW23 and forskolin-stimulated cAMP production after 1 hr by TR-FRET assay
- ChEMBL_797953 (CHEMBL1942830) Antagonist activity at human NPBWR1 expressed in CHO-K1 cells assessed as inhibition of hNPW23 and forskolin-stimulated cAMP production after 1 hr by TR-FRET assay
- ChEMBL_873132 (CHEMBL2182981) Antagonist activity at human mGLUR3 expressed in RGT cells assessed as inhibition of (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid-induced inhibition of forskolin stimulated cyclic-AMP
- ChEMBL_873134 (CHEMBL2182983) Antagonist activity at human mGLUR2 expressed in RGT cells assessed as inhibition of (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid-induced inhibition of forskolin stimulated cyclic-AMP
- ChEMBL_924226 (CHEMBL3075970) Agonist activity at Chilo suppressalis (rice stem borer) tyramine receptor TyR1 expressed in HEK293 cells assessed as reduction in forskolin-induced cAMP accumulation incubated for 20 min
- ChEMBL_943915 (CHEMBL2339882) Agonist activity at recombinant human CB2 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 5 mins by cAMP-competition binding assay
- ChEMBL_943916 (CHEMBL2339883) Agonist activity at recombinant human CB1 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 5 mins by cAMP-competition binding assay
- ChEMBL_946011 (CHEMBL2341542) Inverse agonist activity at human CB2 receptor expressed in CHO cell membranes assessed as inhibition of forskolin-stimulated cAMP production after 45 mins by TR-FRET assay
- ChEMBL_978088 (CHEMBL2422536) Antagonist activity at human histamine H3 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation after 5 hrs by luciferase reporter gene assay
- ChEBML_106043 Tested for agonist activity in non-neuronal cells (RGT) expressing human Metabotropic glutamate receptor 2 by measuring ACPD (3 uM) induced inhibition of forskolin (15 uM)-stimulated cAMP formation
- ChEBML_106381 Tested for agonist activity in non-neuronal cells (RGT) expressing human Metabotropic glutamate receptor 3 by measuring ACPD (3 uM) induced inhibition of forskolin (15 uM)-stimulated cAMP formation
- ChEMBL_143829 (CHEMBL872671) Functional Neuropeptide Y receptor type 1 antagonism was evaluated by its ability to reverse NPY induced inhibition of forskolin-stimulated cyclic AMP production in SK-N-MC cells
- ChEMBL_1465055 (CHEMBL3405211) Agonist activity at GPR88 (unknown origin) transfected in forskolin-stimulated HEK cells assessed as [35S]GTPgammaS binding after 60 mins by trilux scintillation counting analysis relative to control
- ChEMBL_1498162 (CHEMBL3583382) Antagonist activity against human recombinant SSTR3 expressed in CHO cells assessed as reduction in forskolin-induced cAMP accumulation in presence of SS-14 by time-resolved fluorescence assay
- ChEMBL_1518590 (CHEMBL3619405) Antagonist activity at human recombinant mGlu2 receptor expressed in AV12 cells assessed as reversal of DCG-4-inhibited, forskolin-stimulated cAMP production after 1 hr by fluorescence assay
- ChEMBL_1518597 (CHEMBL3619412) Antagonist activity at human recombinant mGlu3 receptor expressed in AV12 cells assessed as reversal of DCG-4-inhibited, forskolin-stimulated cAMP production after 1 hr by fluorescence assay
- ChEMBL_1558359 (CHEMBL3772951) Agonist activity at human Adenosine A1 receptor transfected in CHO-K1 cells assessed as inhibition of forskolin-stimulated cAMP production after 30 mins by multimode microplate reader analysis
- ChEMBL_1558360 (CHEMBL3772952) Agonist activity at human Adenosine A3 receptor transfected in CHO-K1 cells assessed as inhibition of forskolin-stimulated cAMP production after 30 mins by multimode microplate reader analysis
- ChEMBL_1603 (CHEMBL616629) The intrinsic activity was evaluated for its ability to inhibit forskolin-stimulated c-AMP formation mediated by cloned 5-hydroxytryptamine 1B receptor in CHO cell line; Full Agonist
- ChEMBL_1659971 (CHEMBL4009583) Activation of human CFTR expressed in FRT cells co-expressing YFP H148Q mutant assessed as increase in forskolin-stimulated iodide influx measured after 10 mins by fluorescence assay
- ChEMBL_1659972 (CHEMBL4009584) Activation of human CFTR expressed in forskolin-stimulated basolateral membrane permeabilized FRT cells assessed as increase in short-circuit current amplitude after 30 mins by voltage clamp method
- ChEMBL_1757718 (CHEMBL4192726) Agonist activity at human kappa-type opioid receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by luminescence-based assay
- ChEMBL_1843342 (CHEMBL4343769) Agonist activity at recombinant human GPR84 expressed in HEK293 cells co-expressing Gqi5 assessed as inhibition of forskolin-induced cAMP accumulation by fluo-4AM dye based FLIPR assay
- ChEMBL_1843345 (CHEMBL4343772) Agonist activity at recombinant human HA-tagged GPR84 stably expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation measured after 60 mins by HTRF assay
- ChEMBL_2031275 (CHEMBL4685433) Agonist activity at human H3 receptor stably transfected in CHO-DUKX cells assessed as increase in cAMP accumulation by measuring reduction in forskolin level by HitHunter-cAMP assay
- ChEMBL_2051454 (CHEMBL4706153) Agonist activity at NPFFR1 (unknown origin) expressed in HEK293-A cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 30 mins by competition PKA binding assay
- ChEMBL_2051456 (CHEMBL4706155) Agonist activity at NPFFR2 (unknown origin) expressed in HEK293-A cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 30 mins by competition PKA binding assay
- ChEMBL_2064488 (CHEMBL4719741) Agonist activity at NPFFR1 (unknown origin) expressed in HEK293-A cells assessed as reduction in forskolin-induced cAMP accumulation incubated for 30 mins by competition PKA binding assay
- ChEMBL_2064490 (CHEMBL4719743) Agonist activity at NPFFR2 (unknown origin) expressed in HEK293-A cells assessed as reduction in forskolin-induced cAMP accumulation incubated for 30 mins by competition PKA binding assay
- ChEMBL_2075853 (CHEMBL4731387) Agonist activity at human kappa opioid receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation measured after 5 hrs by One-Glo luciferase assay
- ChEMBL_2077207 (CHEMBL4732998) Agonist activity at human delta opioid receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 30 mins by luminescence-based assay
- ChEMBL_2077208 (CHEMBL4732999) Agonist activity at human mu opioid receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 30 mins by luminescence-based assay
- ChEMBL_2077209 (CHEMBL4733000) Agonist activity at human kappa opioid receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 30 mins by luminescence-based assay
- ChEMBL_2091017 (CHEMBL4772280) Agonist activity at human NPFFR2 expressed in CHO cells assessed as inhibition of forskolin-induced intracellular cAMP production incubated for 5 hrs by firefly/renilla luciferase reporter assay
- ChEMBL_2123215 (CHEMBL4832448) Agonist activity at mouse kappa opioid receptor stably expressed in HEK293 cell membrane assessed as inhibition of forskolin induced cAMP production incubated for 30 mins by HTRF assay
- ChEMBL_2154514 (CHEMBL5039174) Agonist activity at 3xHA tagged human CB1 receptor expressed in CHO-K1 cells assessed as reduction in forskolin-stimulated cAMP accumulation incubated for 30 mins by cAMP assay
- ChEMBL_2154515 (CHEMBL5039175) Agonist activity at 3xHA tagged human CB2 receptor expressed in CHO-K1 cells assessed as reduction in forskolin-stimulated cAMP accumulation incubated for 30 mins by cAMP assay
- ChEMBL_2187177 (CHEMBL5099259) Agonist activity at human adenosine A1 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 30 mins by LANCE ultra assay
- ChEMBL_2187180 (CHEMBL5099262) Agonist activity at human adenosine A2A receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 30 mins by LANCE ultra assay
- ChEMBL_2187182 (CHEMBL5099264) Agonist activity at human adenosine A2B receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 30 mins by LANCE ultra assay
- ChEMBL_2187184 (CHEMBL5099266) Agonist activity at human adenosine A3 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 30 mins by LANCE ultra assay
- ChEMBL_2195326 (CHEMBL5107686) Agonist activity at human CB2 receptor expressed in CHO cells assessed as inhibition of accumulation of forskolin-stimulated cAMP level incubated for 35 mins by chemiluminescence based assay
- ChEMBL_2199478 (CHEMBL5111994) Antagonist activity at wild type A3R (unknown origin) expressed in Flp-In-CHO cells assessed as inhibition of NECA reduced forskolin stimulated cAMP accumulation incubated for 30 mins
- ChEMBL_2211900 (CHEMBL5124849) Agonist activity at human A1 receptor expressed in Flp-In-CHO cells assessed as inhibition of forskolin stimulated cAMP accumulation incubated for 30 mins by LANCE cAMP assay
- ChEMBL_2211905 (CHEMBL5124854) Agonist activity at human A2B receptor expressed in Flp-In-CHO cells assessed as cAMP accumulation incubated for 10 mins in presence of forskolin by LANCE cAMP assay
- ChEMBL_2211906 (CHEMBL5124855) Agonist activity at human A3 receptor expressed in Flp-In-CHO cells assessed as cAMP accumulation incubated for 30 mins in presence of forskolin by LANCE cAMP assay
- ChEMBL_2232726 (CHEMBL5146498) Agonist activity at human 5-HT1A receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP production incubated for 40 mins by TR-FRET assay
- ChEMBL_2239292 (CHEMBL5153188) Antagonist activity at P2Y12R (unknown origin) expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 15 mins in presence of IBMX by chromatography
- ChEMBL_2283012 Agonist activity at FFA2 (unknown origin) expressed in human Flp-In-293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 30 mins by Lance cAMP assay
- ChEMBL_2283013 Agonist activity at FFA3 (unknown origin) expressed in human Flp-In-293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 30 mins by Lance cAMP assay
- ChEMBL_2314985 Agonist activity at human D2 receptor stably expressed in CHO-K1 cells assessed as inhibition of forskolin stimulated cAMP production incubated for 30 mins by Lance ultra cAMP assay
- ChEMBL_2314987 Antagonist activity at human D2 receptor stably expressed in CHO-K1 cells assessed as inhibition of forskolin stimulated cAMP production incubated for 30 mins by Lance ultra cAMP assay
- ChEMBL_2314989 Agonist activity at human D4 receptor stably expressed in CHO-K1 cells assessed as inhibition of forskolin stimulated cAMP production incubated for 30 mins by Lance ultra cAMP assay
- ChEMBL_2314991 Antagonist activity at human D4 receptor stably expressed in CHO-K1 cells assessed as inhibition of forskolin stimulated cAMP production incubated for 30 mins by Lance ultra cAMP assay
- ChEMBL_794929 (CHEMBL1936208) Antagonist activity at human adenosine A3 receptor expressed in forskolin-stimulated CHO cells assessed as inhibition of Cl-IB-MECA-induced cAMP production by competition protein binding assay
- ChEMBL_874194 (CHEMBL2187926) Agonist activity at human CB2 receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation using [3H]-cAMP after 5 mins by liquid scintillation counter
- ChEMBL_874195 (CHEMBL2187927) Agonist activity at human CB1 receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation using [3H]-cAMP after 5 mins by liquid scintillation counter
- ChEMBL_910805 (CHEMBL3057561) Agonist activity at Rattus norvegicus (rat) dopamine D2 receptor transfected in african green monkey COS7 cells assessed as inhibition of forskolin-stimulated adenylyl cyclase activity after 10 min
- CB1 cAMP (Assay 2) CHO cells expressing human CB1R (Euroscreen) were plated at a density of 10,000 cells per well in 384 well plates and incubated overnight at 37° C. After removing the media, the cells were treated with test compounds diluted in stimulation buffer containing 1 mM IBMX, 0.25% BSA and 10 uM Forskolin. The assay was incubated for 30 minutes at 37° C. Cells were lysed and the cAMP concentration was measured using DiscoverX-XS cAMP kit, following the manufacturer's protocol. In this setting, agonists will decrease forskolin induced production of cAMP while inverse agonists will further increase forskolin induced production of cAMP. EC50 of agonists were calculated as follows. The maximal amount of cAMP produced by forskolin compared to the level of cAMP inhibited by 1 uM CP55940 is defined as 100%. The EC50 value of each test compound was determined as the concentration at which 50% of the forskolin-stimulated cAMP synthesis was inhibited. Data was analyzed using a four-parameter logistic model. (Model 205 of XLfit 4.0).
- CB2 cAMP (Assay 1) CHO cells expressing human CB2R (Euroscreen) were plated at a density of 10,000 cells per well in 384 well plates and incubated overnight at 37° C. After removing the media, the cells were treated with test compounds diluted in stimulation buffer containing 1 mM IBMX, 0.25% BSA and 10 uM Forskolin. The assay was incubated for 30 minutes at 37° C. Cells were lysed and the cAMP concentration was measured using DiscoverX-XS cAMP kit, following the manufacturer's protocol. In this setting, agonists will decrease forskolin induced production of cAMP while inverse agonists will further increase forskolin induced production of cAMP. EC50 of agonists were calculated as follows. The maximal amount of cAMP produced by forskolin compared to the level of cAMP inhibited by 1 uM CP55940 is defined as 100%. The EC50 value of each test compound was determined as the concentration at which 50% of the forskolin-stimulated cAMP synthesis was inhibited. Data was analyzed using a four-parameter logistic model. (Model 205 of XLfit 4.0).
- In Vitro Assay CB1: CHO cells expressing human CB1R (Euroscreen) were plated at a density of 10,000 cells per well in 384 well plates and incubated overnight at 37° C. After removing the media, the cells were treated with test compounds diluted in stimulation buffer containing 1 mM IBMX, 0.25% BSA and 10 uM Forskolin. The assay was incubated for 30 minutes at 37° C. Cells were lysed and the cAMP concentration was measured using DiscoverX-XS cAMP kit, following the manufacturer's protocol. In this setting, agonists will decrease forskolin induced production of cAMP while inverse agonists will further increase forskolin induced production of cAMP. EC50 of agonists were calculated as follows. The maximal amount of cAMP produced by forskolin compared to the level of cAMP inhibited by 1 uM CP55940 is defined as 100%. The EC50 value of each test compound was determined as the concentration at which 50% of the forskolin-stimulated cAMP synthesis was inhibited. Data was analyzed using a four-parameter logistic model. (Model 205 of XLfit 4.0).
- In Vitro Assay CB2: CHO cells expressing human CB2R (Euroscreen) were plated at a density of 10,000 cells per well in 384 well plates and incubated overnight at 37° C. After removing the media, the cells were treated with test compounds diluted in stimulation buffer containing 1 mM IBMX, 0.25% BSA and 10 uM Forskolin. The assay was incubated for 30 minutes at 37° C. Cells were lysed and the cAMP concentration was measured using DiscoverX-XS cAMP kit, following the manufacturer's protocol. In this setting, agonists will decrease forskolin induced production of cAMP while inverse agonists will further increase forskolin induced production of cAMP. EC50 of agonists were calculated as follows. The maximal amount of cAMP produced by forskolin compared to the level of cAMP inhibited by 1 uM CP55940 is defined as 100%. The EC50 value of each test compound was determined as the concentration at which 50% of the forskolin-stimulated cAMP synthesis was inhibited. Data was analyzed using a four-parameter logistic model. (Model 205 of XLfit 4.0).
- In Vitro Assay CHO cells expressing human CB1 R (Euroscreen) were plated at a density of 10,000 cells per well in 384 well plates and incubated overnight at 37° C. After removing the media, the cells were treated with test compounds diluted in stimulation buffer containing 1 mM IBMX, 0.25% BSA and 10 uM Forskolin. The assay was incubated for 30 minutes at 37° C. Cells were lysed and the cAMP concentration was measured using DiscoverX-XS cAMP kit, following the manufacturer's protocol. In this setting, agonists will decrease forskolin induced production of cAMP while inverse agonists will further increase forskolin induced production of cAMP. EC50 of agonists were calculated as follows. The maximal amount of cAMP produced by forskolin compared to the level of cAMP inhibited by 1 uM CP55940 is defined as 100%. The EC50 value of each test compound was determined as the concentration at which 50% of the forskolin-stimulated cAMP synthesis was inhibited. Data was analyzed using a four-parameter logistic model. (Model 205 of XLfit 4.0).
- In Vitro Assay CHO cells expressing human CB2R (Euroscreen) were plated at a density of 10,000 cells per well in 384 well plates and incubated overnight at 37° C. After removing the media, the cells were treated with test compounds diluted in stimulation buffer containing 1 mM IBMX, 0.25% BSA and 10 uM Forskolin. The assay was incubated for 30 minutes at 37° C. Cells were lysed and the cAMP concentration was measured using DiscoverX-XS cAMP kit, following the manufacturer's protocol. In this setting, agonists will decrease forskolin induced production of cAMP while inverse agonists will further increase forskolin induced production of cAMP. EC50 of agonists were calculated as follows. The maximal amount of cAMP produced by forskolin compared to the level of cAMP inhibited by 1 uM CP55940 is defined as 100%. The EC50 value of each test compound was determined as the concentration at which 50% of the forskolin-stimulated cAMP synthesis was inhibited. Data was analyzed using a four-parameter logistic model. (Model 205 of XLfit 4.0).
- In Vitro Testing of CB1 Potency: CB1 cAMP (Assay 2) CHO cells expressing human CB1R (Euroscreen) were plated at a density of 10,000 cells per well in 384 well plates and incubated overnight at 37° C. After removing the media, the cells were treated with test compounds diluted in stimulation buffer containing 1 mM IBMX, 0.25% BSA and 10 uM Forskolin. The assay was incubated for 30 minutes at 37° C. Cells were lysed and the cAMP concentration was measured using DiscoverX-XS cAMP kit, following the manufacturer's protocol. In this setting, agonists will decrease forskolin induced production of cAMP while inverse agonists will further increase forskolin induced production of cAMP. EC50 of agonists were calculated as follows. The maximal amount of cAMP produced by forskolin compared to the level of cAMP inhibited by 1 uM CP55940 is defined as 100%. The EC50 value of each test compound was determined as the concentration at which 50% of the forskolin-stimulated cAMP synthesis was inhibited. Data was analyzed using a four-parameter logistic model. (Model 205 of XLfit 4.0).
- In Vitro Testing of CB2 Potency: CB2 cAMP (Assay 1) CHO cells expressing human CB2R (Euroscreen) were plated at a density of 10,000 cells per well in 384 well plates and incubated overnight at 37° C. After removing the media, the cells were treated with test compounds diluted in stimulation buffer containing 1 mM IBMX, 0.25% BSA and 10 uM Forskolin. The assay was incubated for 30 minutes at 37° C. Cells were lysed and the cAMP concentration was measured using DiscoverX-XS cAMP kit, following the manufacturer's protocol. In this setting, agonists will decrease forskolin induced production of cAMP while inverse agonists will further increase forskolin induced production of cAMP. EC50 of agonists were calculated as follows. The maximal amount of cAMP produced by forskolin compared to the level of cAMP inhibited by 1 uM CP55940 is defined as 100%. The EC50 value of each test compound was determined as the concentration at which 50% of the forskolin-stimulated cAMP synthesis was inhibited. Data was analyzed using a four-parameter logistic model. (Model 205 of XLfit 4.0).
- ChEBML_106557 Tested for agonist activity in non-neuronal cells (RGT) expressing human Metabotropic glutamate receptor 4 by measuring L-AP4 (3 uM) induced inhibition of forskolin (15 uM)-stimulated cAMP formation
- ChEBML_1685451 Agonist activity at human mu opioid receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by Eu-cAMP tracer based TR-FRET assay
- ChEBML_1685452 Agonist activity at human delta opioid receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by Eu-cAMP tracer based TR-FRET assay
- ChEBML_1685453 Agonist activity at human kappa opioid receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by Eu-cAMP tracer based TR-FRET assay
- ChEBML_2037 Compound was tested for its ability inhibit forskolin-stimulated activity of adenylate cyclase coupled to human 5-hydroxytryptamine 1D receptor beta in CHO-K1 cells; value ranges from 0.24-0.55
- ChEMBL_106043 (CHEMBL717365) Tested for agonist activity in non-neuronal cells (RGT) expressing human Metabotropic glutamate receptor 2 by measuring ACPD (3 uM) induced inhibition of forskolin (15 uM)-stimulated cAMP formation
- ChEMBL_1438388 (CHEMBL3383063) Agonist activity at human recombinant CB2R expressed in WIN-55212-stimulated U2OS cells assessed as reduction in forskolin-mediated cAMP level after 30 mins by time-resolved fluorescence assay
- ChEMBL_1438389 (CHEMBL3383064) Antagonist activity at human recombinant CB2R expressed in WIN-55212-stimulated U2OS cells assessed as increase in forskolin-mediated cAMP level after 30 mins by time-resolved fluorescence assay
- ChEMBL_1472837 (CHEMBL3419499) Antagonist activity against human adenosine A3 receptor expressed in CHO cells assessed as blockade of Cl-IB-MECA-induced inhibition of forskolin-induced cAMP production by scintillation counting method
- ChEMBL_1475512 (CHEMBL3424505) Agonist activity at human somatostatin 4 receptor expressed in H4 cells assessed as inhibition of forskolin-induced intracellular cAMP accumulation incubated for 60 mins by time-resolved fluorescence assay
- ChEMBL_1627041 (CHEMBL3869562) Agonist activity at recombinant human mGlu6 receptor expressed in hamster AV12 cells co-expressing EAAT1 assessed as induction of forskolin-stimulated cAMP formation after 20 mins by HTRF assay
- ChEMBL_1633821 (CHEMBL3876613) Agonist activity at PPLS-HA-tagged-GPR88 (unknown origin) expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production measured after 30 mins by TR-FRET assay
- ChEMBL_1673213 (CHEMBL4023242) Agonist activity at human mGlu6 receptor expressed in hamster AV12 cells co-expressing rat EAAT1 assessed as inhibition of forskolin-stimulated cAMP formation after 20 mins by HTRF assay
- ChEMBL_1714790 (CHEMBL4124839) Antagonist activity at human A1 adenosine receptor expressed in CHO cells assessed as inhibition of CCPA-induced reduction of forskolin-stimulated cAMP level after 30 mins by Alphascreen assay
- ChEMBL_1852077 (CHEMBL4352701) Agonist activity at human Gi/Go-coupled CB1 receptor expressed in CHOK1 cells assessed as inhibition of forskolin-stimulated cAMP production measured after 30 mins by Hit-hunter assay
- ChEMBL_1878382 (CHEMBL4379776) Allosteric agonist activity at N-terminal GFP-tagged rat CB1R expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP production after 30 min by TR-FRET assay
- ChEMBL_198198 (CHEMBL799817) Compound was tested for its ability to inhibit forskolin-stimulated activity of adenylate cyclase coupled to human 5-HT 1A receptor in HeLa cells; value ranges from 110-250
- ChEMBL_198199 (CHEMBL799818) Compound was tested for its ability to inhibit forskolin-stimulated activity of adenylate cyclase coupled to human 5-HT 1A receptor in HeLa cells; value ranges from 50-550
- ChEMBL_2028580 (CHEMBL4682738) Antagonist activity at rat A3AR expressed in CHO cells assessed as reduction in NECA-induced inhibition of forskolin-stimulated cAMP accumulation incubated for 2 hrs by fluorescence based assay
- ChEMBL_2035620 (CHEMBL4689778) Antagonist activity at human EP3 expressed in CHO cells assessed as suppression of sulprostone-induced inhibition of forskolin induced cAMP production measured after 30 mins by HTR-FRET assay
- ChEMBL_2064598 (CHEMBL4719851) Agonist activity at human mu opiod receptor expressed in CHO-K1 cells assessed as increase in forskolin induced cAMP production measured after 30 mins by HitHunter luminescence based assay
- ChEMBL_2064599 (CHEMBL4719852) Agonist activity at human kappa opiod receptor expressed in CHO-K1 cells assessed as increase in forskolin induced cAMP production measured after 30 mins by HitHunter luminescence based assay
- ChEMBL_2064600 (CHEMBL4719853) Agonist activity at human delta opiod receptor expressed in CHO-K1 cells assessed as increase in forskolin induced cAMP production measured after 30 mins by HitHunter luminescence based assay
- ChEMBL_2234640 (CHEMBL5148412) Agonist activity at human APJ receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation measured after 30 mins by TR-FRET based LANCE cAMP assay
- ChEMBL_2239286 (CHEMBL5153182) Antagonist activity at P2Y14R (unknown origin) expressed in rat C6 cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 15 mins in presence of IBMX by chromatography
- ChEMBL_2239287 (CHEMBL5153183) Antagonist activity at P2Y1R (unknown origin) expressed in human 1321N1 cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 15 mins in presence of IBMX by chromatography
- ChEMBL_2239288 (CHEMBL5153184) Antagonist activity at P2Y2R (unknown origin) expressed in human 1321N1 cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 15 mins in presence of IBMX by chromatography
- ChEMBL_2239289 (CHEMBL5153185) Antagonist activity at P2Y4R (unknown origin) expressed in human 1321N1 cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 15 mins in presence of IBMX by chromatography
- ChEMBL_2239290 (CHEMBL5153186) Antagonist activity at P2Y6R (unknown origin) expressed in human 1321N1 cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 15 mins in presence of IBMX by chromatography
- ChEMBL_2239291 (CHEMBL5153187) Antagonist activity at P2Y11R (unknown origin) expressed in human 1321N1 cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 15 mins in presence of IBMX by chromatography
- ChEMBL_2243599 (CHEMBL5157809) Agonist activity at N-terminal 3HA-tagged human CB1 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation measured for 30 mins by FRET assay
- ChEMBL_2253271 (CHEMBL5167481) Antagonist activity at human RXFP3 expressed in CHO-K1 cells assessed as forskolin-induced cAMP accumulation measured after 30 mins in presence of relaxin-3 by TR-FRET assay
- ChEMBL_530927 (CHEMBL969341) Agonist activity at mu opioid receptor assessed as inhibition of forskolin-induced cAMP production in human SH-SY5Y cells pretreated for 24 hrs with DMEM medium after 15 mins
- ChEMBL_530928 (CHEMBL970213) Agonist activity at mu opioid receptor assessed as inhibition of forskolin-induced cAMP production in human SH-SY5Y cells pretreated for 24 hrs with excess ligand after 15 mins
- ChEMBL_607 (CHEMBL615476) Compound was tested for its ability to inhibit forskolin-stimulated activity of adenylate cyclase coupled to human 5-HT 1A receptor in HeLa cells; value ranges from 85-370
- ChEMBL_608 (CHEMBL615477) Compound was tested for its ability to inhibit forskolin-stimulated activity of adenylate cyclase coupled to human 5-hydroxytryptamine 1A receptor in HeLa cells; value ranges from 95-320
- ChEMBL_874120 (CHEMBL2187061) Antagonist activity at human adenosine A3 receptor expressed in CHO cells assessed as blockade of Cl-IB-MECA-mediated inhibition of forskolin-stimulated [3H]cAMP accumulation by scintillation counter
- ChEMBL_941209 (CHEMBL2329720) Inverse agonist activity at human CB2 receptor expressed in CHO cells assessed as increase in forskolin-induced cAMP production after 45 mins in presence of phosphodiesterase inhibitor RO20-1724
- ChEMBL_941739 (CHEMBL2331015) Agonist activity at human GABA-B B1/B2 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 3 hrs by luciferase reporter gene assay
- ChEMBL_958947 (CHEMBL2382644) Antagonist activity at human mGlu6 receptor expressed in golden Syrian hamster AV12 cells coexpressing EAAT1 assessed as inhibition of forskolin-stimulated cAMP production after 20 mins by FLIPR assay
- ChEMBL_958951 (CHEMBL2382648) Antagonist activity at human mGlu3 receptor expressed in golden Syrian hamster AV12 cells coexpressing EAAT1 assessed as inhibition of forskolin-stimulated cAMP production after 20 mins by HTRF assay
- ChEMBL_959165 (CHEMBL2383346) Antagonist activity at human mGlu2 receptor expressed in golden Syrian hamster AV12 cells coexpressing EAAT1 assessed as inhibition of forskolin-stimulated cAMP production after 20 mins by HTRF assay
- ChEMBL_990483 (CHEMBL2446560) Antagonist activity at human histamine H3 receptor expressed in CHO cells assessed as inhibition of forskolin/R-alpha-methylhistamine-induced cAMP accumulation after 1.5 hrs by TR-FRET immunoassay
- ChEMBL_1502795 (CHEMBL3591198) Activity at hemagglutinin-tagged human CB1 receptor expressed in HEK293 cells assessed as effect on forskolin-induced cAMP accumulation after 5 mins in presence of CP55,940 by cAMP BRET assay
- ChEMBL_1502796 (CHEMBL3591199) Activity at hemagglutinin-tagged human CB1 receptor expressed in HEK293 cells assessed as effect on forskolin-induced cAMP accumulation after 10 mins in presence of CP55,940 by cAMP BRET assay
- ChEMBL_1502797 (CHEMBL3591200) Activity at hemagglutinin-tagged human CB1 receptor expressed in HEK293 cells assessed as effect on forskolin-induced cAMP accumulation after 15 mins in presence of CP55,940 by cAMP BRET assay
- ChEMBL_1545049 (CHEMBL3750003) Antagonist activity at human recombinant histamine H3 receptor expressed in HEK293 cells assessed as inhibition of forskolin/(R)(-)-alpha-methylhistamine-induced cAMP accumulation after 1 hr by TR-FRET immunoassay
- ChEMBL_1577394 (CHEMBL3806528) Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay
- ChEMBL_1577396 (CHEMBL3806530) Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay
- ChEMBL_1627045 (CHEMBL3869566) Agonist activity at recombinant human mGlu8 receptor expressed in hamster AV12 cells co-expressing human EAAT1 assessed as induction of forskolin-stimulated cAMP formation after 20 mins by HTRF assay
- ChEMBL_1633824 (CHEMBL3876616) Agonist activity at human PPLS-HA-tagged-GPR88 expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production measured after 30 mins by TR-FRET based LANCE assay
- ChEMBL_1665423 (CHEMBL4015219) Agonist activity at Gi coupled human GPR84 expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 15 mins in presence of [3H]-cAMP by radiometric assay
- ChEMBL_1685452 (CHEMBL4035931) Agonist activity at human delta opioid receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by Eu-cAMP tracer based TR-FRET assay
- ChEMBL_1767247 (CHEMBL4219359) Antagonist activity at recombinant human histamine H3 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated intracellular cAMP accumulation after 30 mins by TR-FRET TR-FRET assay
- ChEMBL_1775527 (CHEMBL4232519) Agonist activity at human GABA-B1/B2 receptor expressed in HEK293/Cre-luc cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 3 hrs by luciferase reporter gene assay
- ChEMBL_1783758 (CHEMBL4255275) Agonist activity at N-terminal HA-tagged human CB2 receptor expressed in HEK293 cells transfected with YFP-Epac-RLuc assessed as decrease in forskolin-stimulated cAMP level by BRET assay
- ChEMBL_1783764 (CHEMBL4255281) Agonist activity at N-terminal HA-tagged human CB1 receptor expressed in HEK293 cells transfected with YFP-Epac-RLuc assessed as decrease in forskolin-stimulated cAMP level by BRET assay
- ChEMBL_1854930 (CHEMBL4355659) Inhibition of human CFTR F508 deletion mutant expressed in rat FRT cells coexpressing YFP-H148Q/I152L 25,22 incubated for 60 to 120 mins in presence of forskolin by fluorescent method
- ChEMBL_1890420 (CHEMBL4392174) Agonist activity at human D2L receptor expressed in CHOK1 cells assessed as inhibition of forskolin-induced cAMP accumulation measured after 30 mins by Eu-cAMP tracer based TR-FRET assay
- ChEMBL_1890424 (CHEMBL4392178) Agonist activity at human D4 receptor expressed in CHOK1 cells assessed as inhibition of forskolin-induced cAMP accumulation measured after 30 mins by Eu-cAMP tracer based TR-FRET assay
- ChEMBL_1914749 (CHEMBL4417332) Agonist activity at human 5HT1A receptor expressed in CHOK1 cells incubated for 16 hrs followed by forskolin-stimulation and measured after 2 hrs by dual-glo luciferase reporter gene assay
- ChEMBL_1915028 (CHEMBL4417611) Antagonist activity at human A1AR expressed in CHO-K1 cells assessed as reduction in NECA-induced inhibition of forskolin-stimulated cAMP accumulation incubated for 30 mins by fluorescence based assay
- ChEMBL_1923544 (CHEMBL4426500) Antagonist activity at human P2Y14R expressed in CHO cells assessed as inhibition of UDPG-mediated reduction of forskolin-induced cAMP production after 15 mins by by competitive enzyme-linked immunoassay
- ChEMBL_1932395 (CHEMBL4478047) Agonist activity at human NPY Y4 receptor expressed in HEK293 cells co-expressing CRE-Luc gene assessed as inhibition of forskolin stimulated luciferase activity after 4.5 hrs by luminescence assay
- ChEMBL_2016540 (CHEMBL4670118) Agonist activity at human NPY2R expressed in CHO cells assessed as inhibition of forskolin-induced intracellular cAMP accumulation incubated for 30 mins in absence of 10% FBS by HTRF assay
- ChEMBL_2016541 (CHEMBL4670119) Agonist activity at human NPY2R expressed in CHO cells assessed as inhibition of forskolin-induced intracellular cAMP accumulation incubated for 30 mins in presence of 10% FBS by HTRF assay
- ChEMBL_2037 (CHEMBL616871) Compound was tested for its ability inhibit forskolin-stimulated activity of adenylate cyclase coupled to human 5-hydroxytryptamine 1D receptor beta in CHO-K1 cells; value ranges from 0.24-0.55
- ChEMBL_2038 (CHEMBL616872) Compound was tested for its ability inhibit forskolin-stimulated activity of adenylate cyclase coupled to human 5-hydroxytryptamine 1D receptor beta in CHO-K1 cells; value ranges from 0.6-2.0
- ChEMBL_2039 (CHEMBL616873) Compound was tested for its ability inhibit forskolin-stimulated activity of adenylate cyclase coupled to human 5-hydroxytryptamine 1D receptor beta in CHO-K1 cells; value ranges from 2.8-6.5
- ChEMBL_2040 (CHEMBL616838) Compound was tested for its ability inhibit forskolin-stimulated activity of adenylate cyclase coupled to human 5-hydroxytryptamine 1D receptor beta in CHO-K1 cells; value ranges from 30-70
- ChEMBL_2046248 (CHEMBL4700947) Activation of human NPFF2R overexpressing CHO cells assessed as inhibition of forskolin-stimulated cAMP degradation incubated for 30 mins in presence of IBMX and 10% FBS by fluorescence based assay
- ChEMBL_2051448 (CHEMBL4706147) Agonist activity at mu opioid receptor (unknown origin) expressed in HEK293-A cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 30 mins by competition PKA binding assay
- ChEMBL_2051450 (CHEMBL4706149) Agonist activity at kappa opioid receptor (unknown origin) expressed in HEK293-A cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 30 mins by competition PKA binding assay
- ChEMBL_2051452 (CHEMBL4706151) Agonist activity at delta opioid receptor (unknown origin) expressed in HEK293-A cells assessed as inhibition of forskolin-stimulated cAMP accumulation incubated for 30 mins by competition PKA binding assay
- ChEMBL_2059300 (CHEMBL4714301) Agonist activity at rat brain CB1 receptor assessed as decrease in forskolin-stimulated cAMP level stimulated for 30 mins followed by Eu-cAMP tracer addition and measured after 60 mins
- ChEMBL_2064481 (CHEMBL4719734) Agonist activity at mu opioid receptor (unknown origin) expressed in HEK293-A cells assessed as reduction in forskolin-induced cAMP accumulation incubated for 30 mins by competition PKA binding assay
- ChEMBL_2064483 (CHEMBL4719736) Agonist activity at kappa opioid receptor (unknown origin) expressed in HEK293-A cells assessed as reduction in forskolin-induced cAMP accumulation incubated for 30 mins by competition PKA binding assay
- ChEMBL_2064485 (CHEMBL4719738) Agonist activity at delta opioid receptor (unknown origin) expressed in HEK293-A cells assessed as reduction in forskolin-induced cAMP accumulation incubated for 30 mins by competition PKA binding assay
- ChEMBL_2090974 (CHEMBL4772237) Antagonist activity at human NPFFR1 expressed in CHO cells assessed as reversal of NPVF-induced inhibition of forskolin-stimulated cAMP accumulation incubated for 10 mins by liquid scintillation counting method
- ChEMBL_2152460 (CHEMBL5037007) Potentiation of CFTR F508del mutant (unknown origin) expressed in CHO cells assessed as chloride transport by measuring membrane potential preincubated for 5 mins followed by forskolin addition by FLIPR assay
- ChEMBL_2193273 (CHEMBL5105633) Agonist activity at human APJ receptor expressed in HEK293 cells assessed as compound stimulated inhibition of forskolin stimulated cAMP production incubated for 30 mins and measured by fluorescence based assay
- ChEMBL_2228637 (CHEMBL5142150) Agonist activity at human melatonin MT1 receptor stably expressing in human HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 30 mins under dark condition by cAMP assay
- ChEMBL_2228641 (CHEMBL5142154) Agonist activity at human melatonin MT2 receptor stably expressing in human HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 30 mins under dark condition by cAMP assay
- ChEMBL_876702 (CHEMBL2182815) Agonist activity at human CB2 receptor expressed in CHO cells assessed as reduction in forskolin-induced cAMP accumulation incubated for 45 mins by TR-FRET method based LANCE cAMP assay
- ChEMBL_1649650 (CHEMBL3998784) Activation of human CFTR expressed in FRT cells co-expressing YFP assessed as increase in forskolin-stimulated iodide influx measured after 10 mins for 2 to 12 sec by fluorescence assay
- ChEMBL_1684927 (CHEMBL4035406) Positive allosteric modulation of human CCR5 expressed in HEK293T cells co-expressing CAMYEL assessed as increase in CCL4-induced inhibition of forskolin-stimulated cAMP accumulation after 10 mins by BRET assay
- ChEMBL_1701164 (CHEMBL4052146) Agonist activity at recombinant human adenosine A1 receptor expressed in CHO cell membranes assessed as inhibition of forskolin-stimulated adenylyl cyclase activity after 10 mins in presence of [alpha-32P]ATP
- ChEMBL_1712045 (CHEMBL4122094) Antagonist activity at human H3 receptor expressed in HEK293 cells assessed as inhibition of RAMH-induced reduction of forskolin-stimulated cAMP accumulation after 30 mins by Ulight-based TR-FRET assay
- ChEMBL_1730605 (CHEMBL4146141) Corrector activity at human CFTR F508-del mutant expressed in rat FTR cells harboring YFP-H148Q/I152L preincubated for 18 to 24 hrs followed by forskolin/genistein addition by HTS assay
- ChEMBL_1755230 (CHEMBL4189990) Antagonist activity at P2Y14R in rat C6 cells assessed as suppression of UDP-glucose-mediated inhibition of forskolin-stimulated [3H]cyclic-AMP accumulation after 15 mins in presence of [3H]-adenine
- ChEMBL_1783759 (CHEMBL4255276) Inverse agonist activity at N-terminal HA-tagged human CB2 receptor expressed in HEK293 cells transfected with YFP-Epac-RLuc assessed as increase in forskolin-stimulated cAMP level by BRET assay
- ChEMBL_1930027 (CHEMBL4433278) Antagonist activity at human H3R expressed in HEK293 cells assessed as reduction in R)(-)-alpha methylhistamine-induced inhibition of forskolin-induced cAMP accumulation by TR-FRET based LANCE ultra cAMP immunoassay
- ChEMBL_1993671 (CHEMBL4627566) Agonist activity at human APJ receptor stably expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation measured after 30 mins by Eu-cAMP tracer based TR-FRET assay
- ChEMBL_2064593 (CHEMBL4719846) Irreversible agonist activity at human adenosine A1 receptor expressed in Flp-In-CHO cells assessed as effect on forskolin-induced cAMP accumulation incubated for 30 mins by LANCE cAMP detection assay
- ChEMBL_2064594 (CHEMBL4719847) Irreversible agonist activity at human adenosine A2A receptor expressed in Flp-In-CHO cells assessed as effect on forskolin-induced cAMP accumulation incubated for 30 mins by LANCE cAMP detection assay
- ChEMBL_2064595 (CHEMBL4719848) Irreversible agonist activity at human adenosine A2B receptor expressed in Flp-In-CHO cells assessed as effect on forskolin-induced cAMP accumulation incubated for 30 mins by LANCE cAMP detection assay
- ChEMBL_2064596 (CHEMBL4719849) Irreversible agonist activity at human adenosine A3 receptor expressed in Flp-In-CHO cells assessed as effect on forskolin-induced cAMP accumulation incubated for 30 mins by LANCE cAMP detection assay
- ChEMBL_2080122 (CHEMBL4735913) Antagonist activity at human histamine H3 receptor expressed in HEK293 cells preincubated for 5 mins followed by forskolin-stimulation and measured after 5 hrs by CRE-driven luciferase reporter gene assay
- ChEMBL_2080125 (CHEMBL4735916) Antagonist activity at human histamine H1 receptor expressed in HEK293 cells preincubated for 5 mins followed by forskolin-stimulation and measured after 5 hrs by CRE-driven luciferase reporter gene assay
- ChEMBL_2080126 (CHEMBL4735917) Antagonist activity at human histamine H2 receptor expressed in HEK293 cells preincubated for 5 mins followed by forskolin-stimulation and measured after 5 hrs by CRE-driven luciferase reporter gene assay
- ChEMBL_2080127 (CHEMBL4735918) Antagonist activity at human histamine H4 receptor expressed in HEK293 cells preincubated for 5 mins followed by forskolin-stimulation and measured after 5 hrs by CRE-driven luciferase reporter gene assay
- ChEMBL_2090971 (CHEMBL4772234) Antagonist activity at human NPFFR1 expressed in CHO-K1 cells assessed as reversal of agonist-induced inhibition of forskolin-stimulated cAMP accumulation incubated for 30 mins by LANCE ultra cAMP assay
- ChEMBL_2090972 (CHEMBL4772235) Antagonist activity at human NPFFR2 expressed in CHO-K1 cells assessed as reversal of agonist-induced inhibition of forskolin-stimulated cAMP accumulation incubated for 30 mins by LANCE ultra cAMP assay
- ChEMBL_2146134 (CHEMBL5030414) Antagonist activity at human EP3 receptor expressed in CHO-K1 cells assessed as reduction in sulprostone induced inhibition of forskolin stimulated cAMP production incubated for 30 mins by TR-FRET assay
- ChEMBL_629910 (CHEMBL1108374) Antagonist activity at human adenosine A3 receptor expressed in forskolin-stimulated CHO cells assessed as inhibition of NSCA-induced decrease in cAMP level pre-incubated for 15 mins by enzyme immunoassay
- ChEMBL_629912 (CHEMBL1108376) Antagonist activity at human adenosine A1 receptor expressed in forskolin-stimulated CHO cells assessed as inhibition of NSCA-induced decrease in cAMP level pre-incubated for 15 mins by enzyme immunoassay
- ChEMBL_754437 (CHEMBL1806025) Antagonist activity at human CXCR4 expressed in human U87 cells expressing CD4 assessed as inhibition of CXCL12-induced cAMP production pretreated for 15 mins before forskolin challenge by TR-FRET analysis
- ChEMBL_757182 (CHEMBL1803406) Antagonist activity human adenosine A3 receptor expressed in CHO cells assessed as blockade of Cl-IB-MECA-mediated inhibition of forskolin-stimulated [3H]cAMP accumulation after 150 mins by scintillation counter
- ChEMBL_817485 (CHEMBL2027708) Antagonist activity at human adenosine A3 receptor expressed in forskolin-stimulated CHO cells assessed as inhibition of NECA-induced CRE-SPAP gene transcription by measuring intracellular cAMP level after 5 hrs
- ChEMBL_876703 (CHEMBL2182816) Inverse agonist activity at human CB2 receptor expressed in CHO cells assessed as increase in forskolin-induced cAMP accumulation incubated for 45 mins by TR-FRET method based LANCE cAMP assay
- ChEMBL_943428 (CHEMBL2341392) Agonist activity at Myc-tagged delta-type opioid receptor (unknown origin) expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 30 mins by liquid scintillation counting analysis
- ChEMBL_943430 (CHEMBL2341394) Agonist activity at FLAG-tagged mu-type opioid receptor (unknown origin) expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 30 mins by liquid scintillation counting analysis
- ChEMBL_1633742 (CHEMBL3876534) Positive allosteric modulation of human mGlu3 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production measured after 30 mins in presence of orthosteric antagonist LY341495 by FLIPR assay
- ChEMBL_1661612 (CHEMBL4011224) Antagonist activity at human dopamine D2 receptor expressed in forskolin stimulated-HEK293 cells coexpressing renilla luciferase-fused Galphai1/GFP10-fused Ggamma2 assessed as inhibition of quinpirole-induced Galphai1 activation by BRET assay
- ChEMBL_1714220 (CHEMBL4124269) Agonist activity at rat mu-opioid receptor expressed in HEK293 cells co-expressing Rluc2-EPAC-GFP10 biosenser assessed as inhibition of forskolin-induced cAMP level after 10 mins by fluorescence based assay
- ChEMBL_1714221 (CHEMBL4124270) Agonist activity at rat delta-opioid receptor expressed in HEK293 cells co-expressing Rluc2-EPAC-GFP10 biosenser assessed as inhibition of forskolin-induced cAMP level after 10 mins by fluorescence based assay
- ChEMBL_1730608 (CHEMBL4146144) Corrector activity at CFTR F508-del mutant (unknown origin) expressed in human CFBE41o cells harboring HS-YFP preincubated for 24 hrs followed by forskolin/genistein stimulation for 30 mins by fluorescence assay
- ChEMBL_1826484 (CHEMBL4326358) Agonist activity at GPR88 (unknown origin) expressed in PPLS-HA-GPR88 CHO cells assessed as effect on forskolin-induced cAMP accumulation after 30 mins by Eu-cAMP tracer-based TR-FRET assay
- ChEMBL_1843340 (CHEMBL4343767) Agonist activity at recombinant human N-terminally HA-tagged GPR84 expressed in CHO cells co-expressing Gqi5 assessed as inhibition of forskolin-induced cAMP accumulation by fluo-4AM dye based FLIPR assay
- ChEMBL_1843346 (CHEMBL4343773) Agonist activity at recombinant human GPR84 expressed in CHO cells co-expressing beta-galactosidase/beta-arrestin2 assessed as inhibition of forskolin-stimulated [3H]cAMP accumulation measured after 15 mins by radioactive assay
- ChEMBL_1973743 (CHEMBL4606561) Inverse agonist activity at human H3R expressed in HEK293T-SP-FLAG-hH3R-CRE-CBR cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assay
- ChEMBL_1973746 (CHEMBL4606564) Inverse agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assay
- ChEMBL_1973750 (CHEMBL4606568) Inverse agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assay
- ChEMBL_2028581 (CHEMBL4682739) Antagonist activity at rat A3AR expressed in CHO cells assessed as reduction in NECA-induced inhibition of forskolin-stimulated cAMP accumulation at 3 uM incubated for 2 hrs by fluorescence based assay
- ChEMBL_2028582 (CHEMBL4682740) Antagonist activity at rat A3AR expressed in CHO cells assessed as reduction in NECA-induced inhibition of forskolin-stimulated cAMP accumulation at 10 uM incubated for 2 hrs by fluorescence based assay
- ChEMBL_2056164 (CHEMBL4711165) Correction activity at CFTR F508del mutant (unknown origin) expressed in CFBE41o- cells coexpressing HS-YFP preincubated for 24 hrs followed by forskolin and genistein addition for 30 mins by fluorescence based assay
- ChEMBL_2111470 (CHEMBL4820320) Positive allosteric modulator activity at human CB2R expressed in CHO-K1 cells assessed as potentiation of CP55940-stimulated inhibition of forskolin-induced cAMP accumulation measured after 90 mins by HitHunter cAMP assay
- ChEMBL_2152462 (CHEMBL5037009) Potentiation of CFTR F508del mutant (unknown origin) expressed in CHO cells assessed as chloride transport by measuring membrane potential incubated for 5 to 30 mins in presence of forskolin by Quattro assay
- ChEMBL_2218766 (CHEMBL5132100) Agonist activity at GPR88 (unknown origin) expressed in CHO cells coexpressing PPLS-HA assessed as reduction in forskolin-stimulated cAMP production and measured after 90 mins by Lance TR-FRET cAMP assay
- ChEMBL_2324631 Antagonist activity at human P2Y14R stably expressed in rat C6 cells assessed as inhibition of forskolin-stimulated cAMP accumulation by measuring inhibition constant incubated for 15 mins in presence of IBMX by chromatography
- ChEMBL_943178 (CHEMBL2346209) Antagonist activity at dopamine D2 receptor (unknown origin) transfected in CHO cell membranes assessed as inhibition of forskolin-stimulated cAMP level after 10 mins by flash plate assay in presence of dopamine
- ChEBML_1573810 Agonist activity at recombinant human adenosine A3 receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation preincubated for 30 mins followed by forsolkin addition measured after 15 mins by immunoassay
- ChEBML_1573812 Agonist activity at mouse adenosine A3 receptor expressed in HEK293 cell membranes assessed as inhibition of forskolin-stimulated cAMP accumulation preincubated for 30 mins followed by forsolkin addition measured after 15 mins by immunoassay
- ChEMBL_1577407 (CHEMBL3806541) Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP production incubated for 6 hrs by CRE/beta-galactosidase reporter gene assay
- ChEMBL_1627029 (CHEMBL3869550) Antagonist activity at recombinant human mGlu3 receptor expressed in hamster AV12 cells co-expressing human EAAT1 assessed as reduction of DCG-4-inhibited forskolin-stimulated cAMP formation after 20 mins by HTRF assay
- ChEMBL_1627030 (CHEMBL3869551) Antagonist activity at recombinant human mGlu2 receptor expressed in hamster AV12 cells co-expressing human EAAT1 assessed as reduction of DCG-4-inhibited forskolin-stimulated cAMP formation after 20 mins by HTRF assay
- ChEMBL_1627040 (CHEMBL3869561) Antagonist activity at recombinant human mGlu6 receptor expressed in hamster AV12 cells co-expressing human EAAT1 assessed as inhibition of L-AP4-inhibited forskolin-stimulated cAMP formation after 20 mins by HTRF assay
- ChEMBL_1822916 (CHEMBL4322680) Agonist activity at wild type human A3 receptor stably expressed in Flp-In CHO cells cotransfected with pOG44 assessed as inhibition of forskolin-induced cAMP accumulation measured after 30 mins by LANCE assay
- ChEMBL_2015972 (CHEMBL4669550) Potentiation of CFTR F508 deletion mutant (unknown origin) expressed in CFBE41o- cells coexpressing HS-YFP assessed as increase in intracellular cAMP content incubated for 20 mins in presence of forskolin by fluorescent method
- ChEMBL_2031278 (CHEMBL4685436) Agonist activity at human H3 receptor expressed in CHO cells assessed as increase in cAMP accumulation by measuring reduction in forskolin level incubated for 4 hrs by CRE/MRE-luciferase reporter gene assay
- ChEMBL_2059302 (CHEMBL4714303) Agonist activity at human CB2 receptor expressed in HEK293 cells assessed as decrease in forskolin-stimulated cAMP level stimulated for 30 mins followed by Eu-cAMP tracer addition and measured after 60 mins
- ChEMBL_2076312 (CHEMBL4731846) Agonist activity at RXFP4 (unknown origin) stably expressed in CHO cells co-transfected with pCRE assessed as inhibition of forskolin-induced cAMP accumulation measured after 6 hrs by beta-galactosidase reporter gene assay
- ChEMBL_2110276 (CHEMBL4818951) Potentiation of CFTR in primary human epithelial enteroid cells assessed as chloride transport in presence of forskolin by measuring membrane potential incubated for 18 to 24 hrs by FLIPR Tetra based dye assay
- ChEMBL_2228638 (CHEMBL5142151) Agonist activity at human melatonin MT1 receptor stably expressing in human HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 30 mins in presence of light-activated compounds by cAMP assay
- ChEMBL_2228642 (CHEMBL5142155) Agonist activity at human melatonin MT2 receptor stably expressing in human HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 30 mins in presence of light-activated compounds by cAMP assay
- ChEMBL_634024 (CHEMBL1117997) Intrinsic activity at mouse histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay
- ChEMBL_793260 (CHEMBL1931709) Antagonist activity at histamine H3 receptor expressed in HEK293 cells co-transfected with pCRE-Luc gene assessed as inhibition of forskolin/histamine-induced cAMP accumulation after 5 hrs by luciferase reporter gene assay
- ChEMBL_793266 (CHEMBL1931715) Antagonist activity at histamine H4 receptor expressed in HEK293 cells co-transfected with pCRE-Luc gene assessed as inhibition of forskolin/histamine-induced cAMP accumulation after 5 hrs by luciferase reporter gene assay
- ChEMBL_941715 (CHEMBL2330938) Inhibition of human A3 adenosine receptor transfected in CHO cells assessed as inhibition of forskolin-induced cyclic AMP production after 10 mins by competitive protein binding assay in presence of CI-IB-MECA
- ChEMBL_1573810 (CHEMBL3803231) Agonist activity at recombinant human adenosine A3 receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation preincubated for 30 mins followed by forsolkin addition measured after 15 mins by immunoassay
- ChEMBL_1573812 (CHEMBL3803233) Agonist activity at mouse adenosine A3 receptor expressed in HEK293 cell membranes assessed as inhibition of forskolin-stimulated cAMP accumulation preincubated for 30 mins followed by forsolkin addition measured after 15 mins by immunoassay
- ChEMBL_1627033 (CHEMBL3869554) Agonist activity at recombinant human mGlu2 receptor expressed in hamster AV12 cells co-expressing human EAAT1 assessed as increase in forskolin-stimulated cAMP formation up to 25 uM after 20 mins by HTRF assay
- ChEMBL_1627034 (CHEMBL3869555) Agonist activity at recombinant human mGlu3 receptor expressed in hamster AV12 cells co-expressing human EAAT1 assessed as increase in forskolin-stimulated cAMP formation up to 25 uM after 20 mins by HTRF assay
- ChEMBL_1731763 (CHEMBL4147299) Neutral antagonist activity at human CB2 assessed as inhibition of WIN552122-induced suppression of cAMP formation preincubated for 15 mins followed by forskolin addition for 30 mins measured after 1 hr by HTRF assay
- ChEMBL_1778803 (CHEMBL4235795) Inhibition of human CCR6 expressed in CHO cells assessed as decrease in CCL20-induced reduction of forskolin-stimulated cAMP accumulation preincubated for 30 mins followed agonist addition measured after 30 mins by HTRF assay
- ChEMBL_1778809 (CHEMBL4235801) Inhibition of monkey CCR6 expressed in CHO cells assessed as decrease in CCL20-induced reduction of forskolin-stimulated cAMP accumulation preincubated for 30 mins followed agonist addition measured after 30 mins by HTRF assay
- ChEMBL_1778810 (CHEMBL4235802) Inhibition of human CCR1 expressed in CHO cells assessed as decrease in RANTES-induced reduction of forskolin-stimulated cAMP accumulation preincubated for 30 mins followed agonist addition measured after 30 mins by HTRF assay
- ChEMBL_1778811 (CHEMBL4235803) Inhibition of human CCR7 expressed in CHO cells assessed as decrease in CCL19-induced reduction of forskolin-stimulated cAMP accumulation preincubated for 30 mins followed agonist addition measured after 30 mins by HTRF assay
- ChEMBL_1861162 (CHEMBL4362018) Antagonist activity at human P2Y14R stably expressed in human forskolin treated THP1 cells assessed as reduction in P2Y14R agonist UDPG-induced inhibition of cAMP production incubated for 15 mins by competitive enzyme-linked immunoassay
- ChEMBL_1870398 (CHEMBL4371565) Agonist activity at dopamine D2 receptor (unknown origin) expressed in HEK293T cells co-expressing luciferase based cAMP biosensor assessed as inhibition of forskolin-mediated cAMP accumulation after 15 mins by microbeta luminescence counting assay
- ChEMBL_1890422 (CHEMBL4392176) Antagonist activity at human D2L receptor expressed in CHOK1 cells assessed as suppression of dopamine-induced inhibition of forskolin-stimulated cAMP accumulation measured after 30 mins by Eu-cAMP tracer based TR-FRET assay
- ChEMBL_1890426 (CHEMBL4392180) Antagonist activity at human D4 receptor expressed in CHOK1 cells assessed as suppression of dopamine-induced inhibition of forskolin-stimulated cAMP accumulation measured after 30 mins by Eu-cAMP tracer based TR-FRET assay
- ChEMBL_1903814 (CHEMBL4406036) Partial agonist activity at recombinant human A3AR expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation measured after 30 mins in presence of A2BAR blocker PSB-603 by Glo-sensor assay
- ChEMBL_2016827 (CHEMBL4670405) Biased agonist activity at human 5-HT1A receptor stably expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation after 40 mins by LANCE Ultra cAMP kit-based TR-FRET assay
- ChEMBL_2076311 (CHEMBL4731845) Agonist activity at RXFP3 (unknown origin) stably expressed in CHO-K1 cells co-transfected with pCRE assessed as inhibition of forskolin-induced cAMP accumulation measured after 6 hrs by beta-galactosidase reporter gene assay
- ChEMBL_2127084 (CHEMBL4836429) Allosteric antagonist activity at N-terminal 3HA-tagged human CB1 receptor expressed in HEK293 cells assessed as reduction in CP55940-stimulated inhibition of forskolin-induced cAMP accumulation measured for 20 mins by BRET assay
- ChEMBL_578610 (CHEMBL1054749) Antagonist activity at human recombinant P2Y12 receptor expressed in CHO FlpIn cells assessed as reduction n 2-methylthio-ADP mediated inhibition of forskolin-induced cAMP response element-directed luciferase expression by bright-glo assay
- ChEMBL_793261 (CHEMBL1931710) Inverse agonist activity at histamine H3 receptor expressed in HEK293 cells co-transfected with pCRE-Luc gene assessed as inhibition of forskolin/histamine-induced cAMP accumulation after 5 hrs by luciferase reporter gene assay
- ChEMBL_943906 (CHEMBL2339467) Partial agonist activity at recombinant human CB1 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation at 100 uM after 5 mins by cAMP-competition binding assay relative to CP55,940
- ChEMBL_943907 (CHEMBL2339468) Partial agonist activity at recombinant human CB2 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation at 100 uM after 5 mins by cAMP-competition binding assay relative to CP55,940
- ChEMBL_1646491 (CHEMBL3995547) Agonist activity at mouse MC1R expressed in HEK293 cells assessed as increase in forskolin-induced cAMP accumulation preincubated for 2 hrs followed by biotinylated cAMP addition and subsequent incubation for 2 hrs by AlphaScreen assay
- ChEMBL_1646492 (CHEMBL3995548) Agonist activity at mouse MC3R expressed in HEK293 cells assessed as increase in forskolin-induced cAMP accumulation preincubated for 2 hrs followed by biotinylated cAMP addition and subsequent incubation for 2 hrs by AlphaScreen assay
- ChEMBL_1646493 (CHEMBL3995549) Agonist activity at mouse MC4R expressed in HEK293 cells assessed as increase in forskolin-induced cAMP accumulation preincubated for 2 hrs followed by biotinylated cAMP addition and subsequent incubation for 2 hrs by AlphaScreen assay
- ChEMBL_1646494 (CHEMBL3995550) Agonist activity at mouse MC5R expressed in HEK293 cells assessed as increase in forskolin-induced cAMP accumulation preincubated for 2 hrs followed by biotinylated cAMP addition and subsequent incubation for 2 hrs by AlphaScreen assay
- ChEMBL_1794849 (CHEMBL4266966) Positive allosteric modulation of recombinant human Gi-coupled mGluR2 expressed in HEK293 Flp-in-T-REx cells with Galpha16 assessed as increase in glutamate-induced forskolin-mediated cAMP accumulation after 5 mins by fluorescence analysis
- ChEMBL_1794850 (CHEMBL4266967) Positive allosteric modulation of recombinant rat Gi-coupled mGluR2 expressed in HEK293 Flp-in-T-REx cells with Galpha16 assessed as increase in glutamate-induced forskolin-mediated cAMP accumulation after 5 mins by fluorescence analysis
- ChEMBL_1902640 (CHEMBL4404862) Agonist activity at human FFA3 receptor stably expressed in Flp-In T-Rex HEK293 cells cotransfected with eYFP assessed as inhibition of forskolin-induced cAMP production measured after 1 hr by fluorescence plate reader assay
- ChEMBL_1904321 (CHEMBL4406543) Agonist activity at human D3R expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production measured after 5 mins in presence of beta-adrenergic blocker propranolol and coelenterazine H by CAMYEL BRET assay
- ChEMBL_1923543 (CHEMBL4426499) Antagonist activity at human P2Y14R expressed in CHO cells assessed as inhibition of UDPG-mediated reduction of forskolin-induced [3H]cAMP production measured after 15 mins in presence of phosphodiesterase inhibitor IBMX by chromatographic method
- ChEMBL_2052448 (CHEMBL4707449) Biased agonist activity at human recombinant 5-HT1A receptor stably expressed in CHO-K1 cells assessed as inhibition of forskolin-induced cAMP accumulation after 40 mins by LANCE Ultra cAMP kit-based TR-FRET assay
- ChEMBL_2074914 (CHEMBL4730448) Potentiation of human CFTR F508 deletion mutant expressed in rat FRT cells coexpressing HS-YFP assessed as increase in iodide influx pre-incubated for 15 to 30 mins in presence of forskolin by fluorescent method
- ChEMBL_2152684 (CHEMBL5037231) Antagonist activity at human adenosine A1 receptor expressed in Flp-In-CHO cell membrane assessed as inhibition of NECA-induced cAMP accumulation in presence of forskolin measured after 40 mins by alpha screen LANCE assay
- ChEMBL_2152685 (CHEMBL5037232) Antagonist activity at human adenosine A2A receptor expressed in Flp-In-CHO cell membrane assessed as inhibition of NECA-induced cAMP accumulation in presence of forskolin measured after 40 mins by alpha screen LANCE assay
- ChEMBL_2152686 (CHEMBL5037233) Antagonist activity at human adenosine A2B receptor expressed in Flp-In-CHO cell membrane assessed as inhibition of NECA-induced cAMP accumulation in presence of forskolin measured after 40 mins by alpha screen LANCE assay
- ChEMBL_2152687 (CHEMBL5037234) Antagonist activity at human adenosine A3 receptor expressed in Flp-In-CHO cell membrane assessed as inhibition of NECA-induced cAMP accumulation in presence of forskolin measured after 40 mins by alpha screen LANCE assay
- ChEMBL_1510268 (CHEMBL3606864) Inverse agonist activity at CB1 receptor (unknown origin) expressed in HEK cells assessed as increase in cAMP production measured at 50 mins by ACTOne dye-based fluorescence assay in presence of forskolin and Ro 20-1724
- ChEMBL_1510270 (CHEMBL3606866) Inverse agonist activity at CB2 receptor (unknown origin) expressed in HEK cells assessed as increase in cAMP production measured at 50 mins by ACTOne dye-based fluorescence assay in presence of forskolin and Ro 20-1724
- ChEMBL_1620896 (CHEMBL3863179) Inverse agonist activity at human CB1 receptor transfected in HEK293 cells assessed as increase of forskolin-induced cAMP production incubated for 30 mins followed by d2 labeled cAMP addition measured after 60 mins by HTRF assay
- ChEMBL_1620897 (CHEMBL3863180) Inverse agonist activity at human CB2 receptor transfected in HEK293 cells assessed as increase of forskolin-induced cAMP production incubated for 30 mins followed by d2 labeled cAMP addition measured after 60 mins by HTRF assay
- ChEMBL_1633737 (CHEMBL3876529) Positive allosteric modulation of human mGlu2 receptor expressed in HEK293 cells coexpressing rat glutamate transporter assessed as inhibition of forskolin-stimulated cAMP production measured after 30 mins in presence of orthosteric antagonist LY341495 by FLIPR assay
- ChEMBL_1870374 (CHEMBL4371541) Agonist activity at full-length Renilla luciferase 8 fused with c-terminal human kappa opioid receptor expressed in HEK293T cells assessed as inhibition of forskolin-mediated cAMP accumulation after 2 mins by BRET based CAMYEL assay
- ChEMBL_2019497 (CHEMBL4673075) Agonist activity at human RXFP4 transfected in CHO-K1 cells co-transfected with pCRE-beta-galactosidase reporter plasmid assessed as inhibition of forskolin-induced cAMP accumulation measured after 6 hrs by beta-galactosidase reporter gene assay
- ChEMBL_2026364 (CHEMBL4680522) Agonist activity at human MOR expressed in HEK293T cells assessed as inhibition of forskolin-stimulated cAMP accumulation preincubated for 5 mins with coelenterazine H followed by compound addition and measured after 15 mins by BRET assay
- ChEMBL_2059301 (CHEMBL4714302) Agonist activity at rat brain CB1 receptor assessed as inhibition of forskolin-stimulated cAMP level at 500 nM stimulated for 30 mins followed by Eu-cAMP tracer addition and measured after 60 mins relative to control
- ChEMBL_2090976 (CHEMBL4772239) Agonist activity at NPFFR1 (unknown origin) expressed in HEK-293A cells assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 30 mins followed by [3H] cAMP addition measured after 2 hrs by scintillation counting method
- ChEMBL_2090977 (CHEMBL4772240) Agonist activity at NPFFR2 (unknown origin) expressed in HEK-293A cells assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 30 mins followed by [3H] cAMP addition measured after 2 hrs by scintillation counting method
- ChEMBL_2222625 (CHEMBL5135959) Modulation of CFTR F508 deletion mutant expressed in HBE cells assessed as change in short circuit current incubated for 18 to 24 hrs in presence of forskolin, amiloride and human serum by voltage-clamp based chamber assay
- ChEMBL_790200 (CHEMBL1925540) Antagonist activity at human glucagon receptor expressed in CHO cells assessed as inhibition of forskolin/glucagon-induced cAMP production preincubated for 30 mins before glucagon challenge measured after 30 mins using [125I]-cAMP by liquid scintillation counting
- ChEMBL_813056 (CHEMBL2020568) Activity at human CFTR delta F508 mutant expressed in forskolin-stimulated FRT cells assessed as increase in iodine influx measured as decrease in YFP fluorescence after 18 to 24 hrs at 37 degC in presence of genistein
- ChEMBL_833910 (CHEMBL2072490) Antagonist activity at human SST3 receptor expressed in CHO cells assessed as inhibition of forskolin/SS-14-stimulated cAMP accumulation preincubated for 15 mins before SS-14 challenge measured after 1 hr by time-resolved fluorescence analysis
- ChEMBL_833917 (CHEMBL2072497) Antagonist activity at mouse SST3 receptor expressed in CHO cells assessed as inhibition of forskolin/SS-14-stimulated cAMP accumulation preincubated for 15 mins before SS-14 challenge measured after 1 hr by time-resolved fluorescence analysis
- ChEMBL_1550686 (CHEMBL3760354) Negative allosteric modulator activity at human CB1R expressed in CHO-K1 cells assessed as effect on CP55,940-induced inhibition of forskolin-induced cAMP accumulation preincubated for 30 mins followed by CP55,940 addition measured for 30 mins by HitHunter assay
- ChEMBL_1556373 (CHEMBL3772104) Agonist activity at recombinant human adenosine A2B receptor assessed as cAMP accumulation preincubated for 45 mins followed by forskolin addition measured after 15 mins by protein kinase A-based assay in presence of [3H]cyclic AMP, rolipram, adenosine deaminase
- ChEMBL_1843339 (CHEMBL4343766) Agonist activity at recombinant human N-terminal epitope tagged/C-terminal eYFP-fused FLAG-tagged GPR84 expressed in Flp-In TREx 293 cell membranes assessed as inhibition of forskolin-induced cAMP production measured after 30 mins by HTRF assay
- ChEMBL_1867091 (CHEMBL4368066) Corrector activity at CFTR F508del mutant (unknown origin) expressed in rat FRT cells assessed as iodide influx preincubated for 18 to 24 hrs followed by compound wash out and addition of forskolin and genistein by fluorescence based assay
- ChEMBL_1867093 (CHEMBL4368068) Potentiator activity at CFTR F508del mutant (unknown origin) expressed in rat FRT cells assessed as iodide influx preincubated for 18 to 24 hrs followed by compound wash out and subsequent compound and forskolin addition by fluorescence based assay
- ChEMBL_1878372 (CHEMBL4379766) Positive allosteric modulatory activity at human CB1R expressed in CHO-K1 cells assessed as increase in CP55940-induced inhibition of forskolin-stimulated cAMP production after 30 mins in presence of CP55940 at EC20 concentration by hit-hunter cAMP assay
- ChEMBL_1878399 (CHEMBL4379793) Positive allosteric modulatory activity at human CB1R expressed in CHO-K1 cells assessed as increase in CP55940-induced inhibition of forskolin-induced cAMP accumulation after 90 mins in presence of 100 nM CP55940 by hit-hunter cAMP assay
- ChEMBL_2090975 (CHEMBL4772238) Agonist activity at kappa opioid receptor (unknown origin) expressed in HEK-293A cells assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 30 mins followed by [3H] cAMP addition measured after 2 hrs by scintillation counting method
- ChEMBL_2123250 (CHEMBL4832483) Positive allosteric modulator activity in mouse kappa opioid receptor stably expressed in HEK293 cell membrane assessed as inhibition of forskolin induced cAMP production by measuring U50,488 EC50 incubated with U50,488 alone measured after 30 mins by HTRF assay
- ChEMBL_2223691 (CHEMBL5137204) Agonist activity at recombinant human SST4 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-stimulated cAMP production measured after 2.5 to 20 hrs in presence of 3-isobutyl-1-methylxanthine by TR-FRET LANCE assay
- ChEMBL_629515 (CHEMBL1120804) Antagonist activity human recombinant adenosine A3 receptor expressed in CHO cells assessed as blockade of NECA-mediated inhibition of forskolin-stimulated [3H]cAMP production treated 15 mins before NECA challenge measured after 30 mins by liquid scintillation spectrometry
- ChEMBL_1793149 (CHEMBL4265068) Antagonist activity at human glucagon receptor expressed in CHO-K1 GCGR Gs cells assessed as inhibition of glucagon-induced cAMP accumulation pre-incubated for 10 mins before glucagon and forskolin addition and further incubated for 30 mins by luminescence-based assay
- ChEMBL_1801543 (CHEMBL4273835) Inhibition of recombinant rat Gi coupled mGlu2 receptor expressed in CHO cells assessed as suppression of L-glutamate-induced decrease in cAMP level pretreated for 1 hr followed by L-glutamate/forskolin addition and measured after 30 mins by FRET assay
- ChEMBL_1867095 (CHEMBL4368070) Corrector activity at human CFTR F508del mutant expressed in rat FRT cells iodide influx incubated for 16 to 20 hrs followed by compound wash out and addition of forskolin and genistein and measured after 20 mins by fluorescence based assay
- ChEMBL_1878384 (CHEMBL4379778) Positive allosteric modulatory activity at N-terminal GFP-tagged rat CB1R receptor expressed in HEK293 cells assessed as CP55940 EC50 for inhibition of forskolin-induced cAMP accumulation at 0.001 microM after 30 mins by TR-FRET assay (Rvb = 22 +/- 6 nM)
- ChEMBL_1878385 (CHEMBL4379779) Positive allosteric modulatory activity at N-terminal GFP-tagged rat CB1R receptor expressed in HEK293 cells assessed as CP55940 EC50 for inhibition of forskolin-induced cAMP accumulation at 0.03 microM after 30 mins by TR-FRET assay (Rvb = 22 +/- 6 nM)
- ChEMBL_1878386 (CHEMBL4379780) Positive allosteric modulatory activity at N-terminal GFP-tagged rat CB1R receptor expressed in HEK293 cells assessed as CP55940 EC50 for inhibition of forskolin-induced cAMP accumulation at 0.1 microM after 30 mins by TR-FRET assay (Rvb = 22 +/- 6 nM)
- ChEMBL_1878387 (CHEMBL4379781) Positive allosteric modulatory activity at N-terminal GFP-tagged rat CB1R receptor expressed in HEK293 cells assessed as CP55940 EC50 for inhibition of forskolin-induced cAMP accumulation at 0.3 microM after 30 mins by TR-FRET assay (Rvb = 22 +/- 6 nM)
- ChEMBL_1931158 (CHEMBL4434409) Agonist activity at S1P1 (unknown origin) expressed in CHO-K1 cells using CC4-AM as substrate assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 4 hrs followed by substrate addition and measured after 2 hrs by FRET assay
- ChEMBL_2223061 (CHEMBL5136395) Antagonist activity at human wild type A1R expressed in CHO-K1 cells assessed as inhibition of forskolin/ NECA-stimulated cAMP accumulation by measuring NECA-mediated cAMP stimulation at 1 uM incubated with NECA for 30 mins by LANCE cAMP detection assay
- ChEMBL_2018600 (CHEMBL4672178) Positive allosteric modulatory activity at human CB1 receptor expressed in CHO-K1 cells assessed as increase in CP55940-induced inhibition of forskolin-stimulated cAMP production preincubated for 30 mins followed by CP55940 addition and measured after 60 mins by Hit-hunter assay
- ChEMBL_2223062 (CHEMBL5136396) Antagonist activity at human wild type A3R expressed in Flp-In-CHO cells assessed as inhibition of forskolin/ NECA-stimulated cAMP accumulation by measuring NECA-mediated cAMP stimulation at 1 uM incubated with NECA for 30 mins by LANCE cAMP detection assay