Query String: levetiracetam
UCB-L059 Keppra BDBM50422542 Levetiracetam In Sodium Chloride LEVETIRACETAM UCB-22059 Keppra Xr
- ChEMBL_2206391 (CHEMBL5119099) Binding affinity to human SV2A in presence of levetiracetam by radioligand binding assay
- ChEMBL_2206392 (CHEMBL5119100) Binding affinity to mouse SV2A in presence of levetiracetam by radioligand binding assay
- ChEMBL_100153 (CHEMBL857182) In vitro inhibitory activity against [3H](2S)-2-[4-(3-azidophenyl)-2-oxopyrrolidin-1-yl]butanamide binding to levetiracetam binding site
- Radioligand Competition Binding Assay For competition binding experiments, 0.5 mg of the rat brain homogenates in 800 μL of binding buffer (2 mM MgCl2 in 50 mM Tris*HCl, pH=7.4) were incubated on a thermoshaker at 37° C. for 30 min with 100 μL (R)-11C-3 or (R)-18F-11 and 100 μL of the compound over a range of concentrations (from 0.1 to 100 nM). Nonspecific binding was defined as the residual binding observed in the presence of 1 mM levetiracetam (100 μL). At completion of the incubation period, the membrane-bound radioligand was recovered using rapid filtration through GF/B glass fiber filters (13 mm GD/X, Whatman Inc.) which were pre-soaked for at least 30 min in 0.5% polyethyleneimine (PEI). The membranes were washed twice with 1 mL of ice-cooled binding buffer. The binding mixtures, filters and filtrates were counted using cross-calibrated gamma counters (1480 & 2480 WIZARD; Perkin-Elmer). Values of IC50 were calculated using the GRAPHPAD PRISM software and converted to inhibition constants (Ki) by the Cheng-Prusoff equation.