| Solution Information | help | |
| Enzyme: | Caspase-7 | |
| inhibitor: | BDBM57513 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this assay is to determine whether compounds confirmed as active in a previous set of experiments entitled, "Absorbance-based confirmation cell-based high throughput confirmation assay for activators of procaspase-3" (PubChem AID 488954), and that were inactive in "Counterscreen for procaspase-3 activators: absorbance-based cell-based high throughput confirmation assay for activators of procaspase-7" (PubChem AID 488941), are nonselective activators due to activation of procaspase 7. This assay also serves to determine procaspase 7 dose response activation curves of tested compounds. This assay employs a procaspase 7 triple-mutant enzyme (D23A/D198A/D206A) (PC-7 D3A) which is unable to autoproteolyze itself because its aspartic acid cleavage sites have been mutated to alanines. The mutant has a fully functional active site that can process the peptidic Ac-DEVD-pNA chromogenic substrate. Cleavage of this substrate by procaspase 7 hydrolyzes the bond betw | |
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