Reaction Details
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Cell Reactant:
Transcriptional Regulator TtgR
Syringe Reactant:
BDBM23446
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
07/23/08
ΔG°:
-10.2±0.1 (kcal/mole)
pH:
7±n/a
Log10Kb:
6.6
Temperature:
303.15±n/a (K)
ΔHobs :
-15.1±0.1 (kJ/mole)
Corrected for ΔHioniz:
no
ΔS° :
-0.02±0 (kJ/mole-K)
Comments:
Thermodynamic parameters for the high affinity binding site; for the low affinity binding site, the K=460000 M-1, deltaG=-7.8 kcal/mol, deltaH=-1.8 kcal/mol.
Citation
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Cell React
Source:
A 651-bp fragment containing the ttgR gene was amplified by PCR from P. putida DOT-T1E chromosomal DNA, and cloned in E. coli expression vector. TtgR protein was over-expressed and purified.
Prep. Method:
TtgR protein was further purified by size exclusion chromatography using a Sephacryl HR-200 column (Amersham Biosciences). Eluted fractions of TtgR were pooled, concentrated, and dialyzed against the buffer.
Name:
Transcriptional Regulator TtgR
Synonyms:
HTH-type transcriptional regulator ttgR | TTGR_PSEPT | Toluene efflux pump ttgABC operon repressor | ttgR
Type:
Repressor; homodimer
Mol. Mass.:
23852.57
Organism:
Pseudomonas putida
Description:
n/a
Residue:
210
Sequence:
MVRRTKEEAQETRAQIIEAAERAFYKRGVARTTLADIAELAGVTRGAIYWHFNNKAELVQALLDSLHETHDHLARASESEDEVDPLGCMRKLLLQVFNELVLDARTRRINEILHHKCEFTDDMCEIRQQRQSAVLDCHKGITLALANAVRRGQLPGELDAERAAVAMFAYVDGLIRRWLLLPDSVDLLGDVEKWVDTGLDMLRLSPALRK