Reaction Details  Report a problem with these data
Cell Reactant:
Heat Shock Protein 90 (Hsp90)
Syringe Reactant:
BDBM15359
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
09/29/09
ΔG°:
-7.75±n/a (kcal/mole)
pH:
8±n/a
Log10Kb:
4.8
Temperature:
283.15±n/a (K)
ΔHobs :
-4.62±0.05 (kJ/mole)
Corrected for ΔHioniz:
no
ΔS° :
0.01±n/a (kJ/mole-K)
Citation
 Nilapwar, S; Williams, E; Fu, C; Prodromou, C; Pearl, LH; Williams, MA; Ladbury, JE Structural-thermodynamic relationships of interactions in the N-terminal ATP-binding domain of Hsp90. J Mol Biol 392:923-36 (2009) [PubMed]  Article
Cell React
Source:
His-tagged N-terminal domain of yeast HSP90 was expressed and purified.
Prep. Method:
Protein was purified by using nickel affinity column, and was subsequently concentrated by an (NH4)2SO4 precipitation and purified further using gel filtration chromatography.
Name:
Heat Shock Protein 90 (Hsp90)
Synonyms:
82 kDa heat shock protein | ATP-dependent molecular chaperone HSP82 | HSP82 | HSP82_YEAST | HSP90 | Heat shock protein Hsp90 heat-inducible isoform
Type:
N-terminal domain
Mol. Mass.:
23389.88
Organism:
Baker's yeast
Description:
P02829 aa 1-207
Residue:
207
Sequence:
MASETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSLSDPKQLETEPDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSGTKAFMEALSAGADVSMIGQFGVGFYSLFLVADRVQVISKSNDDEQYIWESNAGGSFTVTLDEVNERIGRGTILRLFLKDDQLEYLEEKRIKEVIKRHSEFVAYPIQL
  
Syringe React
Name:
BDBM15359
Synonyms:
(4E,6Z,8S,9S,10E,12S,13R,14S,16R)-13-hydroxy-8,14-dimethoxy-4,10,12,16-tetramethyl-3,20,22-trioxo-19-(prop-2-en-1-ylamino)-2-azabicyclo[16.3.1]docosa-1(21),4,6,10,18-pentaen-9-yl carbamate | 17-(Allylamino)geldanamycin | 17-AAG | 17AAG | CHEMBL109480 | GLD-36 | Tanespimycin
Type:
Small organic molecule
Emp. Form.:
C31H43N3O8
Mol. Mass.:
585.6884
SMILES:
CO[C@H]1C[C@H](C)Cc2c(O)c(NC(=O)C(C)=CC=C[C@H](OC)[C@@H](OC(N)=O)\C(C)=C\[C@H](C)[C@H]1O)cc(O)c2N=CC=C |r,w:16.16,38.39,t:28|
Structure:
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