Reaction Details
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Cell Reactant:
Enoyl-ACP Reductase (FabI) Mutant (F203L)
Syringe Reactant:
BDBM3913
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
04/13/05
ΔG°:
-9.23±n/a (kcal/mole)
pH:
7.5±n/a
Log10Kb:
9.1
Temperature:
293.15±n/a (K)
ΔH° :
-11.6±0.79 (kJ/mole)
ΔHobs :
-11.6±0.79 (kJ/mole)
Corrected for ΔHioniz:
no
ΔCp :
-0.91±0.03 (kJ/mole)
Stoich. Param.:
0.61
ΔS° :
-0.01±n/a (kJ/mole-K)
Citation
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Cell React
Source:
The His-tagged FabI (F203L) was expressed and purified from E. coli
Purity:
Purity was not lower than 95%
Prep. Method:
The calorimetric titration of FabI(F203L) was conducted in the presence of 0.5 mM NAD+.
Name:
Enoyl-ACP Reductase (FabI) Mutant (F203L)
Synonyms:
n/a
Type:
Binary complex with a cofactor
Mol. Mass.:
n/a
Description:
n/a
Components:
This complex has 2 components.
Component 1
Name:
Enoyl-ACP Reductase (FabI) Mutant (F203L)
Synonyms:
NADPH-dependent enoyl-ACP reductase
Type:
Enzyme Subunit
Mol. Mass.:
27827.10
Organism:
Escherichia coli
Description:
n/a
Residue:
262
Sequence:
MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKLKGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDGDYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMGLAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDLRKMLAHCEAVTPIRRTVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK
Component 2
Name:
BDBM3909
Synonyms:
NAD+
Type:
Nucleoside or nucleotide
Emp. Form.:
C21H27N7O14P2
Mol. Mass.:
663.4251
SMILES:
NC(=O)c1ccc[n+](c1)[C@@H]1O[C@H](COP([O-])(=O)OP(O)(=O)OC[C@H]2O[C@H]([C@H](O)[C@@H]2O)n2cnc3c(N)ncnc23)[C@@H](O)[C@H]1O |r|