Reaction Details  Report a problem with these data
Cell Reactant:
Major Urinary Protein (MUP-1)
Syringe Reactant:
BDBM12030
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
11/12/06
ΔG°:
-33.9±0.28 (kJ/mole)
pH:
7.4±n/a
Temperature:
308±n/a (K)
ΔHobs :
-44.54±0.4 (kJ/mole)
Corrected for ΔHioniz:
not known
Stoich. Param.:
0.97
ΔS° :
-0.0346±0.0016 (kJ/mole-K)
Citation
 Bingham, RJ; Findlay, JB; Hsieh, SY; Kalverda, AP; Kjellberg, A; Perazzolo, C; Phillips, SE; Seshadri, K; Trinh, CH; Turnbull, WB; Bodenhausen, G; Homans, SW Thermodynamics of Binding of 2-Methoxy-3-isopropylpyrazine and 2-Methoxy-3-isobutylpyrazine to the Major Urinary Protein J Am Chem Soc 126:1675-1681 (2004)    Article
Cell React
Source:
The MUP-1 gene was cloned and expressed in E. coli cells.
Prep. Method:
MUP-I was purified using Ni-NTA resin. To remove endogenous bound ligands, an ethanol precipitation step was utilized involving addition of 2 volumes of absolute ethanol per volume of protein solution, followed by incubation at 4 °C for 2 h. After centrifugation at 5000g, the pellet was lyophilised, resuspended in PBS pH 7.4, and dialyzed overnight against the same buffer.
Name:
Major Urinary Protein (MUP-1)
Synonyms:
MUP1_MOUSE | Major urinary protein 1 precursor | Mup1
Type:
Other Protein Type
Mol. Mass.:
20640.17
Organism:
Mus musculus (mouse)
Description:
n/a
Residue:
180
Sequence:
MKMLLLLCLGLTLVCVHAEEASSTGRNFNVEKINGEWHTIILASDKREKIEDNGNFRLFLEQIHVLENSLVLKFHTVRDEECSELSMVADKTEKAGEYSVTYDGFNTFTIPKTDYDNFLMAHLINEKDGETFQLMGLYGREPDLSSDIKERFAQLCEKHGILRENIIDLSNANRCLQARE
  
Syringe React
Name:
BDBM12030
Synonyms:
2-ISOPROPYL-3-METHOXYPYRAZINE | 2-methoxy-3-(propan-2-yl)pyrazine | IPMP
Type:
Small organic molecule
Emp. Form.:
C8H12N2O
Mol. Mass.:
152.1937
SMILES:
COc1nccnc1C(C)C
Structure:
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