Reaction Details
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Cell Reactant:
Streptavidin
Syringe Reactant:
BDBM12
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
04/30/08
Temperature:
298.15±n/a (K)
ΔHobs :
-102.41±n/a (kJ/mole)
Corrected for ΔHioniz:
no
Stoich. Param.:
0.97
Citation
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Cell React
Source:
Protein was expressed in a T7 expression system (pET-21a, Novagen Inc.,Madison,WI) as insoluble inclusion bodies.
Prep. Method:
Refolded protein was purified by affinity chromatography with iminobiotin-agarose.
Name:
Streptavidin
Synonyms:
SAV_STRAV
Type:
Protein
Mol. Mass.:
18835.54
Organism:
Streptomyces avidinii
Description:
P22629
Residue:
183
Sequence:
MRKIVVAAIAVSLTTVSITASASADPSKDSKAQVSAAEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKPSAASIDAAKKAGVNNGNPLDAVQQ