Cell Reactant:
Streptavidin
Syringe Reactant:
BDBM12
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
04/30/08
Temperature:
298.15±n/a (K)
ΔHobs :
-102.41±n/a (kJ/mole)
Corrected for ΔHioniz:
no
Stoich. Param.:
0.97
Citation
 Chilkoti, ABoyer, FEStayton, PSVara Prasad, JVDomagala, JMEllsworth, ELGajda, CHagen, SEMarkoski, LJTait, BDLunney, EAPalovsky, AFerguson, DGraham, NHoller, THupe, DNouhan, CTummino, PJUrumov, AZeikus, EZeikus, GGracheck, SJSanders, JMVanderRoest, SBrodfuehrer, JIyer, KSinz, MGulnik, SV Molecular Origins of the Slow Streptavidin-Biotin Dissociation Kinetics J Am Chem Soc 117:10622-10628 (1995)    Article
Cell React
Source:
Protein was expressed in a T7 expression system (pET-21a, Novagen Inc.,Madison,WI) as insoluble inclusion bodies.
Prep. Method:
Refolded protein was purified by affinity chromatography with iminobiotin-agarose.
Name:
Streptavidin
Synonyms:
n/a
Type:
Protein
Mol. Mass.:
18835.54
Organism:
Streptomyces avidinii
Description:
P22629
Residue:
183
Sequence:
MRKIVVAAIAVSLTTVSITASASADPSKDSKAQVSAAEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKPSAASIDAAKKAGVNNGNPLDAVQQ
  
Syringe React
Name:
BDBM12
Synonyms:
5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazolidin-4-yl]pentanoic acid | Biotin | cid_171548 | cid_253
Type:
Enzyme co-factor
Emp. Form.:
C10H16N2O3S
Mol. Mass.:
244.311
SMILES:
[H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2 |r|
Structure:
Search PDB for entries with ligand similarity: