Reaction Details  Report a problem with these data
Cell Reactant:
Rho Transcription Termination Factor
Syringe Reactant:
BDBM22600
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
06/14/08
ΔG°:
-25.1218±0.3344 (kJ/mole)
pH:
7.9±n/a
Log10Kb:
3.5
Temperature:
299.15±n/a (K)
ΔHobs :
-3.3022±3.0096 (kJ/mole)
Corrected for ΔHioniz:
no
Stoich. Param.:
6
ΔS° :
0.0729±0.0112 (kJ/mole-K)
Comments:
The stoichiometry parameter was fixed to six, or for the two site model, two integers with a sum of six.
Citation
 Brogan, AP; Widger, WR; Bensadek, D; Riba-Garcia, I; Gaskell, SJ; Kohn, H Development of a technique to determine bicyclomycin-rho binding and stoichiometry by isothermal titration calorimetry and mass spectrometry. J Am Chem Soc 127:2741-51 (2005) [PubMed]  Article
Cell React
Source:
The open reading frame encoding the full-length Escherichia coli Rho protein was PCR amplified and cloned. The protein was overexpressed in E. coli strain BL21 (pLysS).
Purity:
99%
Prep. Method:
The protein was twice purified over a POROS-HS column (Perseptive Biosystems) then further purified with two successive Sephacryl S-300 (Amersham) sizing columns. The protein at this stage was more than 99% pure as judged by SDS-PAGE.
Name:
Rho Transcription Termination Factor
Synonyms:
ATP-dependent helicase rho | RHO_ECOLI | nitA | psuA | rho | rnsC | sbaA | tsu
Type:
Enzyme
Mol. Mass.:
47006.70
Organism:
Escherichia coli (strain K12)
Description:
Rho is a hexameric RNA/DNA helicase/translocase.
Residue:
419
Sequence:
MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAKSGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIRPPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTARVLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTEMQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNTVVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEEFKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGEIDAMEFLINKLAMTKTNDDFFEMMKRS
  
Syringe React
Name:
BDBM22600
Synonyms:
(1S,6R)-6-hydroxy-5-methylidene-1-[(1S,2S)-1,2,3-trihydroxy-2-methylpropyl]-2-oxa-7,9-diazabicyclo[4.2.2]decane-8,10-dione | Bicyclomycin | CGP 354E | FR 1881 | bicozamycin
Type:
Anti-Bacterial Agent
Emp. Form.:
C12H18N2O7
Mol. Mass.:
302.2805
SMILES:
C[C@](O)(CO)[C@H](O)[C@@]12NC(=O)[C@@](O)(NC1=O)C(=C)CCO2 |TLB:10:9:13.14:20.16.19.18,THB:15:14:8.9:20.16.19.18,17:16:13.14:8.9|
Structure:
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