Reaction Details Report a problem with these data
Target
Fatty acid synthase [2202-2509]
Ligand
BDBM24987
Substrate
BDBM24568
Meas. Tech.
Fluorogenic Assay for Detection of FASTE Inhibition
pH
7.5±n/a
Temperature
310.15±n/a K
Ki
850±160 nM
Citation
Richardson, RD; Smith, JW Novel antagonists of the thioesterase domain of human fatty acid synthase. Mol Cancer Ther 6:2120-6 (2007) [PubMed] Article
Target
Name:
Fatty acid synthase [2202-2509]
Synonyms:
FAS | FASN | FAS_HUMAN | Fatty Acid Synthase
Type:
Thioesterase domain
Mol. Mass.:
33927.11
Organism:
Human
Description:
The recombinant thioesterase domain (residues 2202-2509) of FAS was cloned and expressed in Escheria coli. The thioesterase was purified by Ni-affinity chromatography, and analyzed for activity and inhibition by Orlistat.
Residue:
308
Sequence:
CPTPKEDGLAQQQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVR
Inhibitor
Name:
BDBM24987
Synonyms:
(5E)-1-benzyl-5-{[5-(2-chlorophenyl)furan-2-yl]methylidene}-1,3-diazinane-2,4,6-trione | 5-(furan-2-ylmethylene) pyrimidine-2,4,6-trione derivative, 4
Type:
Small organic molecule
Emp. Form.:
C22H15ClN2O4
Mol. Mass.:
406.819
SMILES:
Clc1ccccc1-c1ccc(\C=C2/C(=O)NC(=O)N(Cc3ccccc3)C2=O)o1