Reaction Details
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Ubiquitin carboxyl-terminal hydrolase isozyme L3
Ligand
BDBM65524
Substrate
n/a
Meas. Tech.
Fluorometric Ub-AMC Hydrolysis Assay
pH
7.5±n/a
Temperature
298.15±n/a K
IC50
>114000±n/a nM
Comments
extracted
Citation
Ott, CA; Baljinnyam, B; Zakharov, AV; Jadhav, A; Simeonov, A; Zhuang, Z Cell Lysate-Based AlphaLISA Deubiquitinase Assay Platform for Identification of Small Molecule Inhibitors. ACS Chem Biol 12:2399-2407 (2017) [PubMed] Article More Info.:
Target
Name:
Ubiquitin carboxyl-terminal hydrolase isozyme L3
Synonyms:
UCHL3 | UCHL3_HUMAN | Ubiquitin C-terminal Hydrolase L3 (UCH-L3) | Ubiquitin thioesterase L3
Type:
Hydrolase; protease
Mol. Mass.:
26168.69
Organism:
Homo sapiens (Human)
Description:
Human recombinant UCH-L3 was purchased from Boston Biochem, Inc.(Cambridge, MA).
Residue:
230
Sequence:
MEGQRWLPLEANPEVTNQFLKQLGLHPNWQFVDVYGMDPELLSMVPRPVCAVLLLFPITEKYEVFRTEEEEKIKSQGQDVTSSVYFMKQTISNACGTIGLIHAIANNKDKMHFESGSTLKKFLEESVSMSPEERARYLENYDAIRVTHETSAHEGQTEAPSIDEKVDLHFIALVHVDGHLYELDGRKPFPINHGETSDETLLEDAIEVCKKFMERDPDELRFNAIALSAA
Inhibitor
Name:
BDBM65524
Synonyms:
(-)-Aricine | Heterophylline (VAN) | Quinovatine
Type:
Small organic molecule
Emp. Form.:
C22H26N2O4
Mol. Mass.:
382.4528
SMILES:
COC(=O)C1=CO[C@@H](C)[C@@H]2CN3CCc4c([nH]c5cc(OC)ccc45)[C@@H]3C[C@H]12 |t:4|
Structure:
