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Target
Cathepsin B
Ligand
BDBM16509
Substrate
BDBM16498
Meas. Tech.
Inhibitory Activity Measurement of Bovine Cathepsin B.
pH
6±n/a
Temperature
313.15±n/a K
IC50
38±n/a nM
Comments
The second order rate constant, k2 =112000 M-1s-1.
Citation
 Watanabe, D; Yamamoto, A; Tomoo, K; Matsumoto, K; Murata, M; Kitamura, K; Ishida, T Quantitative evaluation of each catalytic subsite of cathepsin B for inhibitory activity based on inhibitory activity-binding mode relationship of epoxysuccinyl inhibitors by X-ray crystal structure analyses of complexes. J Mol Biol 362:979-93 (2006) [PubMed]  Article 
Target
Name:
Cathepsin B
Synonyms:
BCSB | CATB_BOVIN | CTSB | Cathepsin B precursor | Cathepsin B1
Type:
Enzyme
Mol. Mass.:
36657.87
Organism:
Bos taurus (bovine)
Description:
Bovine spleen CB was purchased from Sigma Co. (USA).
Residue:
335
Sequence:
MWRLLATLSCLLVLTSARSSLYFPPLSDELVNFVNKQNTTWKAGHNFYNVDLSYVKKLCGAILGGPKLPQRDAFAADVVLPESFDAREQWPNCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHSNGRVNVEVSAEDMLTCCGGECGDGCNGGFPSGAWNFWTKKGLVSGGLYNSHVGCRPYSIPPCEHHVNGSRPPCTGEGDTPKCSKTCEPGYSPSYKEDKHFGCSSYSVANNEKEIMAEIYKNGPVEGAFSVYSDFLLYKSGVYQHVSGEIMGGHAIRILGWGVENGTPYWLVGNSWNTDWGDNGFFKILRGQDHCGIESEIVAGMPCTHQY
  
Inhibitor
Name:
BDBM16509
Synonyms:
(2S)-1-[(2S,3S)-3-methyl-2-{[(2S,3S)-3-(propylcarbamoyl)oxiran-2-yl]formamido}pentanoyl]pyrrolidine-2-carboxylic acid | CA074 | CHEMBL490920 | N-{[(2S,3S)-3-(propylcarbamoyl)oxiran-2-yl]carbonyl}-L-isoleucyl-L-proline | PrNH-tES-Ile-Pro-OH | epoxysuccinyl derivative CA074
Type:
Small organic molecule
Emp. Form.:
C18H29N3O6
Mol. Mass.:
383.4394
SMILES:
[H][C@@]1(O[C@]1([H])C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1CCC[C@H]1C(O)=O)C(=O)NCCC |r|
Structure:
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Substrate
Name:
BDBM16498
Synonyms:
Benzyloxycarbonyl (Z)-Phe-Arg 7-(4-methyl)coumarylamide (-NMec) | CBZ-Phe-Arg-AMC | Z-Phe-Arg-NMec | benzyl N-[(1S)-1-{[(1S)-4-carbamimidamido-1-[(4-methyl-2-oxo-2H-chromen-7-yl)carbamoyl]butyl]carbamoyl}-2-phenylethyl]carbamate
Type:
substrate
Emp. Form.:
C33H36N6O6
Mol. Mass.:
612.6755
SMILES:
[#6]-c1cc(=O)oc2cc(-[#7]-[#6](=O)-[#6@H](-[#6]-[#6]-[#6]\[#7]=[#6](\[#7])-[#7])-[#7]-[#6](=O)-[#6@H](-[#6]-c3ccccc3)-[#7]-[#6](=O)-[#8]-[#6]-c3ccccc3)ccc12 |r|
Structure:
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