Reaction Details Report a problem with these data
Target
Peptide deformylase
Ligand
BDBM21685
Substrate
PDF substrate peptide
Meas. Tech.
PDF Inhibition Assay
pH
7.6±n/a
Temperature
298.15±n/a K
IC50
1040±50 nM
Km
4800000±n/a nM
kcat
322±n/a 1/sec
Citation
Smith, KJ; Petit, CM; Aubart, K; Smyth, M; McManus, E; Jones, J; Fosberry, A; Lewis, C; Lonetto, M; Christensen, SB Structural variation and inhibitor binding in polypeptide deformylase from four different bacterial species. Protein Sci 12:349-60 (2003) [PubMed] Article
Target
Name:
Peptide deformylase
Synonyms:
DEF_HAEIN | PDF | Polypeptide deformylase | def
Type:
Enzyme
Mol. Mass.:
19054.46
Organism:
Haemophilus influenzae
Description:
The pdf gene from H. influenzae was cloned and protein was expressed and purified from E. coli BL21(DE3).
Residue:
169
Sequence:
MTALNVLIYPDDHLKVVCEPVTKVNDAIRKIVDDMFDTMYQEKGIGLAAPQVDILQRIITIDVEGDKQNQFVLINPEILASEGETGIEEGCLSIPGFRALVPRKEKVTVRALDRDGKEFTLDADGLLAICIQHEIDHLNGILFVDYLSPLKRQRIKEKLIKYKKQIAKS
Inhibitor
Name:
BDBM21685
Synonyms:
N-hydroxy-N-[3-(6-methylpyridin-2-yl)propyl]formamide | SB-505684
Type:
Small organic molecule
Emp. Form.:
C10H14N2O2
Mol. Mass.:
194.2304
SMILES:
Cc1cccc(CCCN(O)C=O)n1
Substrate
Name:
PDF substrate peptide
Synonyms:
n/a
Type:
Peptide
Mol. Mass.:
1718.01
Organism:
n/a
Description:
n/a
Residue:
14
Sequence:
FRMYLMETALASER