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Target
DNA polymerase alpha subunit B
Ligand
BDBM21686
Substrate
BDBM21687
Meas. Tech.
DNA Polymerase Activity Assay
pH
7.5±n/a
Temperature
310.15±n/a K
Ki
>1008000±n/a nM
Citation
 Butler, MM; Lamarr, WA; Foster, KA; Barnes, MH; Skow, DJ; Lyden, PT; Kustigian, LM; Zhi, C; Brown, NC; Wright, GE; Bowlin, TL Antibacterial activity and mechanism of action of a novel anilinouracil-fluoroquinolone hybrid compound. Antimicrob Agents Chemother 51:119-27 (2007) [PubMed]  Article 
Target
Name:
DNA polymerase alpha subunit B
Synonyms:
70 kD subunit | DNA Polymerase alpha | DNA polymerase alpha 70 kDa subunit | DNA polymerase alpha subunit B | DPOA2_BOVIN | POLA2 | Polymerase (DNA directed), alpha 2
Type:
Enzyme Subunit
Mol. Mass.:
66940.74
Organism:
Bos taurus (bovine)
Description:
Enzyme was purchased from Ulrich Hubscher, University of Zurich-Irchel, Switzerland.
Residue:
604
Sequence:
MAVSAQLLVEELQIFGLECEEAVIEKLVELCILYGQNEEGMASELIAFCTSTRKDCFTLETLNSFEHEFLSKRVSKTRHGASKDKGLRHAGARDIVSIQELIEVEEEEETLLNSYTTPSKGSQKRTITTPETPLTKRSVSARSPHQLLSPSSFSPSATPPQKYSSRSNRGEVVTSFGSAQGVSWSGRGGASPLSLKVLGHPEPLTGSYKYMFQKLPDIREVLTCKIEELGSELKEHYKIEAFAPILVPAQEPVTLLGQIGCDSNGKLNHKSVILEGDLEHSSGAQIPVDLSELKEYSLFPGQVVVMEGINTTGRKLVATRLYEGVPLPFHQPDEEDGDSEQFMVLVACGPYTTSDSITFDPLLDLITIINRDRPDVCILFGPFLDAKHEQVESCLLTSSFEDVFKQCLRTIIEGTRSSGSHLIIVPSLRDVHHEPVYPQPPFSCSDLLREDKKRVRLVSEPCTLSINGVIFGLTSTDLLFHMGAEEISSSSGTSDRFSRILRHILTQRSYYPLYPPQEDMAIDYENFYLYAQLPVTPDVFIAPSELRYFVKVGLNSAISLQCQNQFLSGALALNSSRVCWWGQWPRSFGGGVGVENKSVSIMLK
  
Inhibitor
Name:
BDBM21686
Synonyms:
3-(4-hydroxybutyl)-6-(3-ethyl-4-methylanilino)uracil | 6-[(3-ethyl-4-methylphenyl)amino]-3-(4-hydroxybutyl)-1,2,3,4-tetrahydropyrimidine-2,4-dione | CHEMBL327687 | HB-EMAU | HBEMAU
Type:
Small organic molecule
Emp. Form.:
C17H23N3O3
Mol. Mass.:
317.3828
SMILES:
CCc1cc(Nc2cc(=O)n(CCCCO)c(=O)[nH]2)ccc1C
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
BDBM21687
Synonyms:
({[({[(2R,3S,5R)-5-(2-amino-6-oxo-6,9-dihydro-3H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy}(hydroxy)phosphoryl)oxy](hydroxy)phosphoryl}oxy)phosphonic acid | dGTP | deoxyguanosine triphosphate
Type:
Nucleoside or nucleotide
Emp. Form.:
C10H16N5O13P3
Mol. Mass.:
507.181
SMILES:
Nc1nc2n(cnc2c(=O)[nH]1)[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1
Structure:
Search PDB for entries with ligand similarity: