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Target
Ubiquitin carboxyl-terminal hydrolase isozyme L3
Ligand
BDBM23421
Substrate
BDBM23422
Meas. Tech.
Enzyme Inhibition Assay
pH
7.8±n/a
Temperature
295.15±n/a K
IC50
103000±n/a nM
Km
83.3±1.5 nM
Citation
 Hirayama, K; Aoki, S; Nishikawa, K; Matsumoto, T; Wada, K Identification of novel chemical inhibitors for ubiquitin C-terminal hydrolase-L3 by virtual screening. Bioorg Med Chem 15:6810-8 (2007) [PubMed]  Article 
Target
Name:
Ubiquitin carboxyl-terminal hydrolase isozyme L3
Synonyms:
UCHL3 | UCHL3_HUMAN | Ubiquitin C-terminal Hydrolase L3 (UCH-L3) | Ubiquitin thioesterase L3
Type:
Hydrolase; protease
Mol. Mass.:
26168.69
Organism:
Homo sapiens (Human)
Description:
Human recombinant UCH-L3 was purchased from Boston Biochem, Inc.(Cambridge, MA).
Residue:
230
Sequence:
MEGQRWLPLEANPEVTNQFLKQLGLHPNWQFVDVYGMDPELLSMVPRPVCAVLLLFPITEKYEVFRTEEEEKIKSQGQDVTSSVYFMKQTISNACGTIGLIHAIANNKDKMHFESGSTLKKFLEESVSMSPEERARYLENYDAIRVTHETSAHEGQTEAPSIDEKVDLHFIALVHVDGHLYELDGRKPFPINHGETSDETLLEDAIEVCKKFMERDPDELRFNAIALSAA
  
Inhibitor
Name:
BDBM23421
Synonyms:
1-benzyl-3-hydroxy-4-[(5-methylfuran-2-yl)carbonyl]-5-(pyridin-3-yl)-2,5-dihydro-1H-pyrrol-2-one | ChemBridge CNS-Set 7504601, 1
Type:
Small organic molecule
Emp. Form.:
C22H18N2O4
Mol. Mass.:
374.3893
SMILES:
Cc1ccc(o1)C(=O)C1C(N(Cc2ccccc2)C(=O)C1=O)c1cccnc1
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
BDBM23422
Synonyms:
Ub-AMC | ubiquitin-7-amido-4-methylcoumarin
Type:
Fluorogenic substrate
Emp. Form.:
n/a
Mol. Mass.:
n/a
SMILES:
n/a
Structure: