Reaction Details Report a problem with these data
Target
Ubiquitin carboxyl-terminal hydrolase isozyme L3
Ligand
BDBM23425
Substrate
BDBM23422
Meas. Tech.
Enzyme Inhibition Assay
pH
7.8±n/a
Temperature
295.15±n/a K
IC50
>331000±n/a nM
Km
83.3±1.5 nM
Citation
 Hirayama, K; Aoki, S; Nishikawa, K; Matsumoto, T; Wada, K Identification of novel chemical inhibitors for ubiquitin C-terminal hydrolase-L3 by virtual screening. Bioorg Med Chem 15:6810-8 (2007) [PubMed]  Article 
Target
Name:
Ubiquitin carboxyl-terminal hydrolase isozyme L3
Synonyms:
UCHL3 | UCHL3_HUMAN | Ubiquitin C-terminal Hydrolase L3 (UCH-L3) | Ubiquitin thioesterase L3
Type:
Hydrolase; protease
Mol. Mass.:
26168.69
Organism:
Homo sapiens (Human)
Description:
Human recombinant UCH-L3 was purchased from Boston Biochem, Inc.(Cambridge, MA).
Residue:
230
Sequence:
MEGQRWLPLEANPEVTNQFLKQLGLHPNWQFVDVYGMDPELLSMVPRPVCAVLLLFPITEKYEVFRTEEEEKIKSQGQDVTSSVYFMKQTISNACGTIGLIHAIANNKDKMHFESGSTLKKFLEESVSMSPEERARYLENYDAIRVTHETSAHEGQTEAPSIDEKVDLHFIALVHVDGHLYELDGRKPFPINHGETSDETLLEDAIEVCKKFMERDPDELRFNAIALSAA
  
Inhibitor
Name:
BDBM23425
Synonyms:
ChemBridge CNS-Set 7909542, 5 | N-{3-[1-acetyl-5-(thiophen-2-yl)-4,5-dihydro-1H-pyrazol-3-yl]phenyl}ethane-1-sulfonamide
Type:
Small organic molecule
Emp. Form.:
C17H19N3O3S2
Mol. Mass.:
377.481
SMILES:
CCS(=O)(=O)Nc1cccc(c1)C1=NN(C(C1)c1cccs1)C(C)=O |t:13|
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
BDBM23422
Synonyms:
Ub-AMC | ubiquitin-7-amido-4-methylcoumarin
Type:
Fluorogenic substrate
Emp. Form.:
n/a
Mol. Mass.:
n/a
SMILES:
n/a
Structure: