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Target
Calpain-1 catalytic subunit
Ligand
BDBM23859
Substrate
BDBM23856
Meas. Tech.
mu-Calpain Inhibition Assay
pH
7.4±n/a
Temperature
295.15±n/a K
Ki
41±7 nM
Citation
 Donkor, IO; Assefa, H; Liu, J Structural basis for the potent calpain inhibitory activity of peptidyl alpha-ketoacids. J Med Chem 51:4346-50 (2008) [PubMed]  Article 
Target
Name:
Calpain-1 catalytic subunit
Synonyms:
CAN1_PIG | CANP 1 | CAPN1 | Calcium-activated neutral proteinase 1 | Calpain 1 | Calpain mu-type | Calpain-1 | Calpain-1 catalytic subunit | Calpain-1 large subunit | Micromolar-calpain | muCANP
Type:
Catalytic subunit; forms a heterodimer with a small (regulatory) subunit (CAPNS1)
Mol. Mass.:
81729.82
Organism:
Sus scrofa (pig)
Description:
Native calpain-1 from porcine erythrocytes, purchased from Calbiochem, was used in assay.
Residue:
714
Sequence:
MAEEVITPVYCTGVSAQVQKLRAKELGLGRHENAIKYLGQDYEQLRAHCLQSGSLFRDEAFPPVPQSLGFKELGPNSSKTYGVKWKRPTELFSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQNGYAGIFHFQLWQFGEWVDVVVDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYELRKAPSDLYSIILKALERGSLLGCSIDISSVLDMEAVTFKKLVKGHAYSVTGAKQVNYQGQMVNLIRMRNPWGEVEWTGAWSDGSSEWNGVDPYQRDQLRVRMEDGEFWMSFRDFLREFTRLEICNLTPDALKSQRVRNWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEETDDPEDDYGGRESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKKAGTQELDDQVQAILPDEQVLSEEEIDENFKALFRQLAGEDMEISVRELRTILNRIISKHKDLRTKGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIESAGFKLNKKLFELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA
  
Inhibitor
Name:
BDBM23859
Synonyms:
3-[(2S)-2-[1-(adamantan-1-yl)acetamido]-4-methylpentanamido]-2-oxo-4-phenylbutanoic acid | Peptidyl alpha-Ketoacid, 2b
Type:
Small organic molecule
Emp. Form.:
C28H38N2O5
Mol. Mass.:
482.6117
SMILES:
CC(C)C[C@H](NC(=O)CC12CC3CC(CC(C3)C1)C2)C(=O)NC(Cc1ccccc1)C(=O)C(O)=O |r,TLB:16:11:18:15.14.17,16:15:11.12.10:18,THB:14:13:10:15.16.17,14:15:10:13.12.18|
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
BDBM23856
Synonyms:
Suc-LY-AMC (AMC=7-Amino-4-methylcoumarin) | Suc-Leu-Tyr-AMC
Type:
Fluorogenic substrate
Emp. Form.:
n/a
Mol. Mass.:
n/a
SMILES:
n/a
Structure: