Reaction Details Report a problem with these data
Target
Dimer of Gag-Pol polyprotein [501-599,D531N,M537I]
Ligand
BDBM518
Substrate
Chromogenic peptide substrate
Meas. Tech.
Protease Inhibition Assay
pH
4.7000±n/a
Temperature
310.1500±n/a K
Ki
6±1 nM
Km
22000.0000±2000.0000 nM
kcat
4.9000±0.3000 1/sec
Citation
 Clemente, JC; Hemrajani, R; Blum, LE; Goodenow, MM; Dunn, BM Secondary mutations M36I and A71V in the human immunodeficiency virus type 1 protease can provide an advantage for the emergence of the primary mutation D30N. Biochemistry 42:15029-35 (2003) [PubMed]  Article 
Target
Name:
Dimer of Gag-Pol polyprotein [501-599,D531N,M537I]
Synonyms:
HIV-1 Protease Mutant (D30N/M36I)
Type:
Protein Complex
Mol. Mass.:
n/a
Description:
n/a
Components:
This complex has 2 components.
Component 1
Name:
Gag-Pol polyprotein [501-599,D531N,M537I]
Synonyms:
HIV-1 Protease Mutant (D30N/M36I) chain A | HIV-1 Protease Mutant (D30N/M36I) chain B | LAI (D30N/M36I) | POL_HV1BR | gag-pol
Type:
Enzyme Subunit
Mol. Mass.:
10777.17
Organism:
Human immunodeficiency virus type 1
Description:
P03367[501-599,D531N,M537I]
Residue:
99
Sequence:
PQITLWQRPLVTIKIGGQLKEALLDTGADNTVLEEISLPGRWKPKMIGGIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNF
  
Component 2
Name:
Gag-Pol polyprotein [501-599,D531N,M537I]
Synonyms:
HIV-1 Protease Mutant (D30N/M36I) chain A | HIV-1 Protease Mutant (D30N/M36I) chain B | LAI (D30N/M36I) | POL_HV1BR | gag-pol
Type:
Enzyme Subunit
Mol. Mass.:
10777.17
Organism:
Human immunodeficiency virus type 1
Description:
P03367[501-599,D531N,M537I]
Residue:
99
Sequence:
PQITLWQRPLVTIKIGGQLKEALLDTGADNTVLEEISLPGRWKPKMIGGIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNF
  
Inhibitor
Name:
BDBM518
Synonyms:
Viracept | CHEMBL584 | (3S,4aS,8aS)-N-tert-butyl-2-[(2R,3R)-2-hydroxy-3-[(3-hydroxy-2-methylphenyl)formamido]-4-(phenylsulfanyl)butyl]-decahydroisoquinoline-3-carboxamide | AG-1343 | Nelfinavir
Type:
Small organic molecule
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
Chromogenic peptide substrate
Synonyms:
Chromogenic peptide substrate
Type:
Peptide
Mol. Mass.:
1420.11
Organism:
n/a
Description:
n/a
Residue:
13
Sequence:
KARVLNPHEANLG