Reaction Details Report a problem with these data
Target
Serine/threonine-protein kinase-transforming protein raf [E280V]
Ligand
BDBM81374
Substrate
n/a
Meas. Tech.
In Vitro Activity Assay
pH
7.5±0
Kd
15±2 nM
Citation
 Ranjitkar, P; Brock, AM; Maly, DJ Affinity reagents that target a specific inactive form of protein kinases. Chem Biol 17:195-206 (2010) [PubMed]  Article 
Target
Name:
Serine/threonine-protein kinase-transforming protein raf [E280V]
Synonyms:
RAF_MSV36 | Tyrosine-protein kinase SRC (E280V) | V-RAF
Type:
Protein
Mol. Mass.:
59986.38
Organism:
Gallus gallus
Description:
P00532[E280V]
Residue:
533
Sequence:
MGSSKSKPKDPSQRRRSLEPPDSTHHGGFPASQTPNKTAAPDTHRTPSRSFGTVATEPKLFGGFNTSDTVTSPQRAGALAGGVTTFVALYDYESRTETDLSFKKGERLQIVNNTEGDWWLAHSLTTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNPENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFSSLQQLVAYYSKHADGLCHRLTNVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGVVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKGEMGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKDPEERPTFEYLQAFLEDYFTSTEPQYQPGENL
  
Inhibitor
Name:
BDBM81374
Synonyms:
BODIPY-labeled probe, 4a
Type:
Small organic molecule
Emp. Form.:
C50H49BF4N11O4
Mol. Mass.:
954.799
SMILES:
Cc1cc(C)n2B(F)[N+]3=C(CCC(=O)NCCCCC(=O)NCCOc4ccc(Nc5ncnc(n5)-c5cccnc5Nc5cc(NC(=O)c6cccc(c6)C(F)(F)F)ccc5C)cc4)C=CC3=Cc12 |c:8,70,73|
Structure:
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