Reaction Details
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Cyclin-dependent kinase 1/G2/mitotic-specific cyclin-B
Ligand
BDBM5580
Substrate
Histone H1
Meas. Tech.
Kinase Inhibition Assay
Citation
Mesguiche, V; Parsons, RJ; Arris, CE; Bentley, J; Boyle, FT; Curtin, NJ; Davies, TG; Endicott, JA; Gibson, AE; Golding, BT; Griffin, RJ; Jewsbury, P; Johnson, LN; Newell, DR; Noble, ME; Wang, LZ; Hardcastle, IR 4-Alkoxy-2,6-diaminopyrimidine derivatives: inhibitors of cyclin dependent kinases 1 and 2. Bioorg Med Chem Lett 13:217-22 (2003) [PubMed] Article More Info.:
Target
Name:
Cyclin-dependent kinase 1/G2/mitotic-specific cyclin-B
Synonyms:
CDK1/Cyclin B | Cyclin-Dependent Kinase 1 (CDK1) | p34cdc2/Cyclin B
Type:
Protein Complex
Mol. Mass.:
n/a
Description:
p34cdc2/cyclin B was purified from M-phase starfish oocytes by affinity chromatography.
Components:
This complex has 2 components.
Component 1
Name:
Cyclin-dependent kinase 1
Synonyms:
CDK1_ORYLA | Cell division control protein 2 (cdc2) | P34 protein kinase | cdc2 | cdk1
Type:
Enzyme Subunit
Mol. Mass.:
34693.35
Organism:
Marthasterias glacialis (starfish)
Description:
Q9DGD3 {empty hard to find)
Residue:
303
Sequence:
MEDYVKIEKIGEGTYGVVYKGRHKSTGQVVAMKKIRLESEEEGVPSTAVREVSLLQELKHPNVVRLLDVLMQESRLYLIFEFLSMDLKKYLDSIPSGQYMDPMLVKSYLYQILEGIYFCHRRRVLHRDLKPQNLLIDNKGVIKLADFGLARAFGVPVRVYTHEVVTLWYRAPEVLLGSPRYSTPVDVWSTGTIFAELATKKPLFHGDSEIDQLFRIFRTLGTPNNDVWPDVESLPDYKNTFPKWKEGSLSSMVKNLDKNGLDLLAKMLIYNPPKRISAREAMTHPYFDDLDKSTLPAACINGV
Component 2
Name:
G2/mitotic-specific cyclin-B
Synonyms:
CCNB_MARGL | Cyclin B | G2/mitotic-specific cyclin-B1
Type:
Enzyme Subunit
Mol. Mass.:
43790.28
Organism:
Marthasterias glacialis (starfish)
Description:
n/a
Residue:
388
Sequence:
MLNGENVDSRIMGKVATRASSKGVKSTLGTRGALENISNVARNNLQAGAKKELVKAKRGMTKSKATSSLQSVMGLNVEPMEKAKPQSPEPMDMSEINSALEAFSQNLLEGVEDIDKNDFDNPQLCSEFVNDIYQYMRKLEREFKVRTDYMTIQEITERMRSILIDWLVQVHLRFHLLQETLFLTIQILDRYLEVQPVSKNKLQLVGVTSMLIAAKYEEMYPPEIGDFVYITDNAYTKAQIRSMECNILRRLDFSLGKPLCIHFLRRNSKAGGVDGQKHTMAKYLMELTLPEYAFVPYDPSEIAAAALCLSSKILEPDMEWGTTLVHYSAYSEDHLMPIVQKMALVLKNAPTAKFQAVRKKYSSAKFMNVSTISALTSSTVMDLADQMC
Inhibitor
Name:
BDBM5580
Synonyms:
6-(cyclohexylmethoxy)-4-N-(4-methoxyphenyl)-5-nitrosopyrimidine-2,4-diamine | pyrimidine deriv. 8b
Type:
Small organic molecule
Emp. Form.:
C18H23N5O3
Mol. Mass.:
357.4069
SMILES:
COc1ccc(Nc2nc(N)nc(OCC3CCCCC3)c2N=O)cc1
Structure:
Substrate
Name:
Histone H1
Synonyms:
Sigma Type IIIS
Type:
Other Protein Type
Mol. Mass.:
358.43
Organism:
Bos taurus (bovine)
Description:
12.5 uM ATP as co-substrate.
Residue:
3
Sequence:
NA