| Assay Method Information | |
| | SARS-CoV-2 Coronavirus 3C Protease FRET Assay |
| Description: | SARS-CoV-2 Mpro (final concentration 750 nM) plus every compound in 25 uL of buffer (20 mM Tris-HCl, pH 7.5, 150mM NaCl，1mM EDTA，2mM DTT) is incubated for 10 minutes. Enzyme reactions were initiated with the addition of 25 uL of 25 uM fluorescent substrate (MCA-AVLQ SGFR-Lys(Dnp)-Lys-NH2). The fluorescence was measured (excitation 365 nm, emission 445 nm) in fluorimeter. IC50 values were calculated by fitting a curve of Vmax to experimental values with the controls (DMSO) as the top and bottom limits of the curve. The curve consists of 9 concentrations of the compound; and the IC50 value is an average of 3 measurements. GraphPad Prism is the analysis tool for all measurements. |
| Affinity data for this assay | |
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