Assay Method Information | |
| Fluorescence Peptide Cleavage Assay |
Description: | Fluorescence peptide cleavage assay was performed in a 96-well plate. Each reaction contained MAPKKide, LF, and the small-molecule inhibitor. The C-terminally linked fluorophore of MAPKKide was a FITC, and the acceptor chromophore was 4-([4-(dimethylamino)-phenyl] azo) benzoic acid (DABCYL). Cleavage of the substrate by LF released the fluorophore and full fluorescence was restored. The reaction was examined by using a fluorescent plate reader at excitation and emission wavelength at 485 and 590 nm. |
Affinity data for this assay | |
---|---|
If you find an error in this entry please send us an E-mail |