| Assay Method Information | |
| | MDH1 and MDH2 Inhibitory Activity Assay |
| Description: | The inhibitory activity of the prepared compounds against MDH1 recombinant protein and the MDH2 recombinant proteins prepared in each of Experimental Examples 1-1, 1-2, 1-3 and 1-4 was measured. More specifically, 0.25 nM MDH1 recombinant protein (Biovision) or MDH2 recombinant protein was added to 200 μM oxaloacetate, nicotinamide adenine dinucleotide (NADH), the compound prepared in Example 1 and an MDH assay buffer (100 mM potassium phosphate, pH 7.4) to allow a reaction for 30 minutes. Afterward, a change in NADH concentration in the solution due to NADH oxidation (NAD+) by an MDH1 or MDH2 enzyme was analyzed by measuring absorbance at 340 nm. |
| Affinity data for this assay | |
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