Assay Method Information

Assay Name:  Fluorescence Anisotropy Assay
Description:  The assay was adapted to be able to measure the high affinity of compounds for galectin-3 by using the below probe constructed to have high affinity for galectin-3 which made it possible to use a low concentration of galectin-3 (50 nM). 100 nM albumin was included as a carrier to prevent protein loss at such low concentration of galectin.
Affinity data for this assay
 

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