| Assay Method Information | |
| | Evaluation of RAF Kinase Activity |
| Description: | ormula (I) bis-hydrochloride salt prepared in Example 2 (hereinafter referred to as Compound⋅2HCl ) was tested for inhibitory activity against three subtypes of RAF, i.e., RAF1 (C-RAF)Y340D/Y341D, B-RAF wild type and B-RAFV600E using Kinase Profiling Service (Thermo Fisher Scientific, previously Invitrogen, U.S.A) according to the manufacturer's instructions. The levels of enzymatic inhibition of the compound were calculated as percent inhibition at various concentrations. Based on percent inhibition, dose-response curves were plotted using GraphPad Prism software. |
| Affinity data for this assay | |
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