| Assay Method Information | |
| | Inhibitory Action on Binding of BRD4 Protein and Ligand Test Method 1 |
| Description: | Table 4-1: The inhibitory action of the compound of the present invention on the binding of BRD4 protein and a ligand thereof was evaluated by the time-resolved fluorescence resonance energy transfer (TR-FRET) method using BRD4 bromodomain 1 TR-FRET Assay Kit (Cayman). In this experiment, (+)-JQ1 known to have an inhibitory activity on the binding of BRD4 protein and a ligand therefor was used as a positive control drug. A test compound dissolved in DMSO was diluted with TR-FRET Assay Buffer, added to a 384 well black plate, BRD4 bromodomain 1 Europium Chelete was further added and the cells were incubated at room temperature for 15 min. Thereafter, BRD4 bromodomain 1 Ligand/APC Acceptor Mixture was added and the cells were incubated at room temperature for 1 hr. The binding amount of the BRD4 protein and ligand was measured as fluorescence intensity (excitation: 340 nm/emission: 620 nm and excitation: 340 nm/emission: 670 nm). |
| Affinity data for this assay | |
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