| Assay Method Information | |
| | Proteasome Inhibitory Activity |
| Description: | The proteasome used in the present invention is human erythrocyte 20S proteasome, and the enzyme, fluorescent substrate and test buffer are all purchased from Enzo. The experimental system is 16 μL, of which the substrate is 8 μL; the proteasome is 4 μL (0.8 ng) and has a final concentration of 50 μM; the test agent (inhibitor) is 4 μL, and has a final concentration of 2×10−6 M-4.88×10−10 M, and the last concentration is 0 M, where the actually formulated concentration is 8×10−6M-1.95×10−9M, and the last concentration is 0 M. |
| Affinity data for this assay | |
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