Assay Method Information | |
| Determination of Compound's ROCK Inhibitory Activity In Vitro (Z'Lyte Assay) |
Description: | Recombinant ROCK1 (amino acids 1-535) and ROCK2 (amino acids 1-552) proteins were purchased from ThermoFisher Scientific. Compound's activities were measured by Z′-lyte kinase kit (ThermoFisher Scientific) and IC50 was calculated |
Affinity data for this assay | |
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