| Assay Method Information | |
| | Inhibition of rhGAA Determined by Measuring the Residual Activity in the Presence of an Inhibitor in Vitro |
| Description: | The rhGAA enzyme sold under the name Myozyme used comes from residues of perfusions of the recombinant enzyme used for treating, by enzyme therapy, the patients with Pompe disease in the Department of Translational Medical Sciences, University of Naples Federico II (IT). The compounds are solubilized at various concentrations in a 100 mM sodium acetate buffer, pH 4.0, as well as the 4-nitrophenyl-α-D-glucopyranoside substrate (20 mM). After 2 minutes of equilibration of the temperature at 37 C., the rhGAA enzyme in solution in the same buffer is added (total volume: 200 μL). After 2 minutes of reaction at 37 C., a 1M solution of sodium carbonate (800 μL), pH 11.0, is added and the mixture is cooled in ice. The absorbance of the solution is measured at 420 nm at ambient temperature. |
| Affinity data for this assay | |
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